| 质粒名称: | pcDNA-dCas9 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 47106 |
| 存储实验室: | Charles Gersbach |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 9575 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9 |
| 物种来源: | S. Pyogenes |
| 插入大小: | 4236 bp |
| 基因突变: | D10A, H840A (catalytically inactive) |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
标志（插入时N端子）
SV40 NLS（N端子插入）
SV40 NLS（C端子插入）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA-dCas9 was a gift from Charles Gersbach (Addgene plasmid # 47106 ; http://n2t.net/addgene:47106 ; RRID:Addgene_47106) |
| 参考文献部分: | RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. 10.1038/nmeth.2600 PubMed 23892895 |
买家导航
pcDNA-dCas9载体 pcDNAdCas9质粒基本信息
pcDNA-dCas9质粒图谱 pcDNAdCas9载体图谱和pcDNA-dCas9载体序列 pcDNAdCas9质粒序列多克隆位点信息
pcDNA-dCas9质粒 pcDNAdCas9载体简介
pcDNA-dCas9质粒序列 pcDNAdCas9载体序列
LOCUS pcDNA-dCas9 9575 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian
cells.
ACCESSION .
VERSION .
KEYWORDS pcDNA-dCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9575)
AUTHORS Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein
LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford
GE, Reddy TE, Gersbach CA
TITLE RNA-guided gene activation by CRISPR-Cas9-based transcription
factors.
JOURNAL Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
PUBMED 23892895
REFERENCE 2 (bases 1 to 9575)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9575
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 907..972
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 979..999
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1009..5112
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 5113..5133
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(5169..5186)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5175..5399
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5445..5873
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5532..5551)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5742..5763
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5882..5902)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5887..6216
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6067..6202
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6129..6148
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6283..7077
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6337..6356)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6947..6966
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7251..7372
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7288..7307)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7342..7361
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7421..7437)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7421..7437)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7434..7456)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7445..7461
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7469..7499)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7514..7535
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7652..7669)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7823..8408)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7903..7922)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8579..9439)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9202..9221
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9440..9544)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
961 gacgatgaca agatggcccc caagaagaag aggaaggtgg gccgcggaat ggataagaaa
1021 tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat cactgatgaa
1081 tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa
1141 aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc
1201 aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag
1261 attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct
1321 tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat
1381 gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat
1441 tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt
1501 cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta
1561 tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt
1621 ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat
1681 ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg
1741 tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta
1801 cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca aattggagat
1861 caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat
1921 atcctaagag taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc
1981 tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca
2041 gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat
2101 gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat
2161 ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc
2221 tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga
2281 agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg
2341 acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg
2401 atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa
2461 ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat
2521 gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg
2581 acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag
2641 aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta
2701 aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa
2761 gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa
2821 gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc
2881 ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat
2941 gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga
3001 aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa
3061 tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt
3121 aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt
3181 gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt
3241 gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca
3301 cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc
3361 gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact
3421 caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg
3481 gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat tgttccacaa
3541 agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt
3601 ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga
3661 caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa
3721 cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact
3781 cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat
3841 gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct
3901 gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc
3961 catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt
4021 gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag
4081 tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac
4141 ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa
4201 actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc
4261 aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga
4321 ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa
4381 gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta
4441 gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta
4501 gggatcacaa ttatggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct
4561 aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt
4621 gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat
4681 gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag
4741 ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat
4801 tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat
4861 gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa
4921 caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt
4981 aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat
5041 gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag
5101 ctaggaggtg accccaagaa gaagaggaag gtgggctgat ctagacttaa gtttaaaccg
5161 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
5221 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
5281 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
5341 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
5401 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
5461 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
5521 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
5581 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
5641 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
5701 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
5761 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
5821 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
5881 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
5941 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
6001 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
6061 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
6121 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
6181 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
6241 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
6301 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
6361 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
6421 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
6481 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
6541 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
6601 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
6661 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
6721 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
6781 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
6841 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
6901 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
6961 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
7021 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
7081 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
7141 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
7201 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
7261 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
7321 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
7381 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
7441 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
7501 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
7561 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
7621 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
7681 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
7741 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
7801 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
7861 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
7921 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
7981 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
8041 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
8101 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
8161 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
8221 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
8281 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
8341 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
8401 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
8461 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
8521 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
8581 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
8641 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
8701 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
8761 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
8821 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
8881 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
8941 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
9001 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
9061 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
9121 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
9181 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
9241 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
9301 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
9361 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
9421 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
9481 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
9541 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
pcDNA-dCas9载体图谱 pcDNAdCas9质粒图谱pdf版和pcDNA-dCas9载体序列 pcDNAdCas9质粒序列等相关资料下载
pcDNA-dCas9质粒 pcDNAdCas9载体应用举例
- 上一篇：pSaCas9_GFP
- 下一篇：pCRISPRainbow-DONOR1
