| 质粒名称: | pAAV-CAG-DIO-Ace-mNeon2-Kv2.1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000570 |
| 产品用途: | to express Ace-mNeon2-Kv2.1 in Cre positive mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Ace-mNeon2-Kv2.1 |
| 物种来源: | mouse |
| 密码子优化: | 老鼠表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and Photostable Voltage Sensors Derived from mBaoJin
Zhang H., Zhou S., Kuzmicheva T.P., Subach O.M., Grimm C., Eom M., Baror-Sebban S., Adam Y., Yoon Y.G., Emilliani V., Subach F.V., Piatkevich K.D.
bioRxiv 2025.05.30.657123 (2025)
|
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pAAV-CAG-DIO-Ace-mNeon2-Kv2.1载体质粒基本信息
pAAV-CAG-DIO-Ace-mNeon2-Kv2.1质粒图谱载体图谱和pAAV-CAG-DIO-Ace-mNeon2-Kv2.1载体序列质粒序列多克隆位点信息
pAAV-CAG-DIO-Ace-mNeon2-Kv2.1质粒载体简介
pAAV-CAG-DIO-Ace-mNeon2-Kv2.1质粒序列载体序列
LOCUS pAAV-CAG-DIO-Ace-mNeon2-Kv2.1 2398 bp DNA linear SYN 14-MAY-2024
DEFINITION synthetic linear DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2398)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..2398
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 2..44
/label=WPRE-R
protein_bind complement(68..101)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind complement(172..205)
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
primer_bind 198..250
/label=0430-DIO-F
misc_feature 225..230
/label=Kozak sequence
CDS 231..917
/codon_start=1
/label=AceNR
/translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
ETIFKSY"
primer_bind 774..793
/label=Ace-F
primer_bind complement(888..917)
/label=1104-1-Ace-R
primer_bind complement(888..917)
/label=0806-1-Ace-PR-R
primer_bind 898..941
/label=1104-2-mNeonGreen-F
CDS 918..1586
/codon_start=1
/product="bright monomeric yellow-green fluorescent protein
derived from LanYFP (Shaner et al., 2013)"
/label=mNeonGreen
/translation="PATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQF
SPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYE
GSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKR
YRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELY
K"
regulatory 1134..1143
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1563..1606)
/label=1104-2-mNeonGreen-R
CDS 1587..1646
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
primer_bind 1587..1606
/label=1104-3-KGC-F
CDS 1647..1667
/codon_start=1
/label=ER2
/translation="FCYENEV"
CDS 1668..1862
/codon_start=1
/label=Kv2.1
/translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
MSSIDSFISCATDFPEATRF"
primer_bind complement(1843..1891)
/label=0430-DIO-R
primer_bind complement(1843..1864)
/label=Kv2.1-Rv-ER2
protein_bind 1890..1923
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind 1986..2019
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
ORIGIN
1 caaattttgt aatccagagg ttgattatcg ataagcttga tatcgaattc gaacctcttc
61 gagggacata acttcgtata atgtatgcta tacgaagtta tgggtcgatg gtgagatctg
121 gactagaggg tcgatggtga tgcttggatc aaaataggaa gaatcccaat gataacttcg
181 tataaagtat cctatacgaa gttatactag aattcggtac ctaagccacc atggctgacg
241 tggaaaccga gaccggcatg attgcacagt ggattgtctt tgctattatg gctgctgctg
301 ctattgcttt tggagtggct gtgcactttc ggccttcaga gctgaagagc gcatactata
361 tcaacattgc catctgcact atcgccgcta ccgcttacta tgcaatggcc gtgaactacc
421 aggacctgac aatgaatggt gaaaggcagg tggtctacgc aagatatatt tcctgggtgc
481 tgaccacacc actgctcctg ctcgatctca tcgtcatgac caagatgggc ggagtgatga
541 tttcttgggt catcggcgca gacattttca tgatcgtgtt tggtattctg ggcgccttcg
601 aggatgaaca caagttcaaa tgggtgtact ttatcgctgg atgtgtgatg caggcagtcc
661 tgacatacgg gatgtataac gccacttgga aagacgatct gaagaaaagc cccgagtacc
721 atagctccta tgtcagtctg ctcgtcttcc tgtcaatcct ctgggtgttt tatcctgtcg
781 tgtgggcttt cgggtctggt agtggcgtgc tgtccgtcga caatgaggcc attctcatgg
841 gaatcctgga tgtgctcgct aagccactgt ttggaatggg gtgcctcatt gcccatgaga
901 ctatcttcaa gtcctaccca gcgacacatg agttacacat ctttggctcc atcaacggtg
961 tggactttga catggtgggt cagggcaccg gcaatccaaa tgatggttat gaggagttaa
1021 acctgaagtc caccaagggt gacctccagt tctccccctg gattctggtc cctcatatcg
1081 ggtatggctt ccatcagtac ctgccctacc ctgacgggat gtcgcctttc caggccgcca
1141 tggtagatgg ctccggctac caagtccatc gcacaatgca gtttgaagat ggtgcctccc
1201 ttactgttaa ctaccgctac acctacgagg gaagccacat caaaggagag gcccaggtga
1261 aggggactgg tttccctgct gacggtcctg tgatgaccaa ctcgctgacc gctgcggact
1321 ggtgcaggtc gaagaagact taccccaacg acaaaaccat catcagtacc tttaagtgga
1381 gttacaccac gggaaatggc aagcgctacc ggagcactgc gcggaccacc tacacctttg
1441 ccaagccaat ggcggctaac tatctgaaga accagccgat gtacgtgttc cgtaagacgg
1501 agctcaagca ctccaagacc gagctcaact tcaaggagtg gcaaaaggcc tttaccgatg
1561 tgatgggcat ggacgagctg tataagaaga gcaggatcac cagcgagggc gagtacatcc
1621 ccctggacca gatcgacatc aacgtgttct gctacgagaa cgaggtgcag agccagccta
1681 tcctgaacac caaggagatg gcccctcaga gcaagcctcc tgaggagctg gagatgagca
1741 gcatgcctag ccctgtggcc cctctgcctg ccaggaccga gggcgtgatc gacatgagga
1801 gcatgagcag catcgacagc ttcatcagct gcgccaccga cttccctgag gccaccaggt
1861 tctaatgtac aagtaagggg atccctagta taacttcgta tagcatacat tatacgaagt
1921 tatgggtcga tggtgatgct tggcaattcg ggtcgatggt gaagcattgg tcttcctatt
1981 ttgatataac ttcgtatagg atactttata cgaagttatg gggatccaat tctttgccaa
2041 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
2101 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
2161 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga gccgctcccc
2221 ccgcacaaag ggccctcccg gagcccctca aggctttcac gcagccacag aaaagaaaca
2281 agccgtcatt aaaccaagcg ctaattacag cccggaggag aagggccgtc ccgcccgctc
2341 acctgtggga gtaacgcggt cagtcagagc cggggcggcg gggccccaaa ggcggcgc
//
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