pAT03-Apr

LOCUS       pAT03-Apr               11230 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  For curing of the resistance marker of RecET recombinase system in 
            Acinetobacter baumannii, Aprar.
ACCESSION   .
VERSION     .
KEYWORDS    pAT03-Apr
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Sun B, Liu H, Jiang Y, Shao L, Yang S, Chen D
  TITLE     New Mutations Involved in Colistin Resistance in Acinetobacter 
            baumannii.
  JOURNAL   mSphere. 2020 Apr 1;5(2). pii: 5/2/e00895-19. doi: 
            10.1128/mSphere.00895-19.
  PUBMED    32238571
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(38..55)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      208..294
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      386..413
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        432..523
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(742..761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(962..1762)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2954..3348
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            3690..3777
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             5016..5987
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6498..7337
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(8452..9534)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9496..9515
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9535..9612)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        9842..9870
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    9878..9894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9883..9905
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             9940..11211
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
ORIGIN
        1 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
       61 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
      121 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
      181 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      241 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
      301 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
      361 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
      421 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      481 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      541 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      601 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      661 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      721 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
      781 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      841 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      901 catgagtgat aacactgcgg ccaacttact cctgacaacg gaataggaac ttatgagctc
      961 agccaatcga ctggcgagcg gcatcgcatt cttcgcatcc cgcctctggc ggatgcagga
     1021 agatcaacgg atctcggccc agttgaccca gggctgtcgc cacaatgtcg cgggagcgga
     1081 tcaaccgagc aaaggcatga ccgactggac cttccttctg aaggctcttc tccttgagcc
     1141 acctgtccgc caaggcaaag cgctcacagc agtggtcatt ctcgagataa tcgacgcgta
     1201 ccaacttgcc atcctgaaga atggtgcagt gtctcggcac cccataggga acctttgcca
     1261 tcaactcggc aagatgcagc gtcgtgttgg catcgtgtcc cacgccgagg agaagtacct
     1321 gcccatcgag ttcatggaca cgggcgaccg ggcttgcagg cgagtgaggt ggcaggggca
     1381 atggatcaga gatgatctgc tctgcctgtg gccccgctgc cgcaaaggca aatggatggg
     1441 cgctgcgctt tacatttggc aggcgccaga atgtgtcaga gacaactcca aggtccggtg
     1501 taacgggcga cgtggcagga tcgaacggct cgtcgtccag acctgaccac gagggcatga
     1561 cgagcgtccc tcccggaccc agcgcagcac gcagggcctc gatcagtcca agtggcccat
     1621 cttcgagggg ccggacgcta cggaaggagc tgtggaccag cagcacaccg ccgggggtaa
     1681 ccccaaggtt gagaagctga ccgatgagct cggcttttcg ccattcgtat tgcacgacat
     1741 tgcactccac cgctgatgac atcagtcgat catagcacga tcaacggcac tgttgcaaat
     1801 agtcggtggt gataaactta tcatcccctt ttgcttatgg agctgcacat gaacccattc
     1861 aaaggccggc attttcagcg tgacatcatt ctgtgggccg tacgctggta ctgcaaatac
     1921 ggcatcagtt accgtgagct gcattttccg ctgcataacc ctgcttcggg gtcattatag
     1981 cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc
     2041 gtgtagactt tccttggtgt atccaacggc gtcagcggag gaccgaagga gctaaccgct
     2101 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2161 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2221 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2281 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2341 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2401 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2461 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2521 tcagaccaag tttactcata tatactttag attgatttct gaaagcgacc aggtgctcgg
     2581 cgtggcaaga ctcgcagcga acccgtagaa agccatgctc cagccgcccg cattggagaa
     2641 attcttcaaa ttcccgttgc acatagcccg gcaattcctt tccctgctct gccataagcg
     2701 cagcgaatgc cgggtaatac tcgtcaacga tctgatagag aagggtttgc tcgggtcggt
     2761 ggctctggta acgaccagta tcccgatccc ggctggccgt cctggccgcc acatgaggca
     2821 tgttccgcgt ccttgcaata ctgtgtttac atacagtcta tcgcttagcg gaaagttctt
     2881 ttaccctcag ccgaaatgcc tgccgttgct agacattgcc agccagtgcc cgtcactccc
     2941 gtactaactg tcacgaaccc ctgcaataac tgtcacgccc ccctgcaata actgtcacga
     3001 acccctgcaa taactgtcac gcccccaaac ctgcaaaccc agcaggggcg ggggctggcg
     3061 gggtgttgga aaaatccatc catgattatc taagaataat ccactaggcg cggttatcag
     3121 cgcccttgtg gggcgctgct gcccttgccc aatatgcccg gccagaggcc ggatagctgg
     3181 tctattcgct gcgctaggct acacaccgcc ccaccgctgc gcggcagggg gaaaggcggg
     3241 caaagcccgc taaaccccac accaaacccc gcagaaatac gctggagcgc ttttagccgc
     3301 tttagcggcc tttcccccta cccgaagggt gggggcgcgt gtgcagcccc gcagggcctg
     3361 tctcggtcga tcattcagcc cggctcatcc ttctggcgtg gcggcagacc gaacaaggcg
     3421 cggtcgtggt cgcgttcaag gtacgcatcc attgccgcca tgagccgatc ctccggccac
     3481 tcgctgctgt tcaccttggc caaaatcatg gcccccacca gcaccttgcg ccttgtttcg
     3541 ttcttgcgct cttgctgctg ttcccttgcc cgcacccgct gaatttcggc attgattcgc
     3601 gctcgttgtt cttcgagctt ggccagccga tccgccgcct tgttgctccc cttaaccatc
     3661 ttgacacccc attgttaatg tgctgtctcg taggctatca tggaggcaca gcggcggcaa
     3721 tcccgaccct actttgtagg ggagggcgca cttaccggtt tctcttcgag aaactggcct
     3781 aacggccacc cttcgggcgg tgcgctctcc gagggccatt gcatggagcc gaaaagcaaa
     3841 agcaacagcg aggcagcatg gcgatttatc accttacggc gaaaaccggc agcaggtcgg
     3901 gcggccaatc ggccagggcc aaggccgact acatccagcg cgaaggcaag tatgcccgcg
     3961 acatggatga agtcttgcac gccgaatccg ggcacatgcc ggagttcgtc gagcggcccg
     4021 ccgactactg ggatgctgcc gacctgtatg aacgcgccaa tgggcggctg ttcaaggagg
     4081 tcgaatttgc cctgccggtc gagctgaccc tcgaccagca gaaggcgctg gcgtccgagt
     4141 tcgcccagca cctgaccggt gccgagcgcc tgccgtatac gctggccatc catgccggtg
     4201 gcggcgagaa cccgcactgc cacctgatga tctccgagcg gatcaatgac ggcatcgagc
     4261 ggcccgccgc tcagtggttc aagcggtaca acggcaagac cccggagaag ggcggggcac
     4321 agaagaccga agcgctcaag cccaaggcat ggcttgagca gacccgcgag gcatgggccg
     4381 accatgccaa ccgggcatta gagcgggctg gccacgacgc ccgcattgac cacagaacac
     4441 ttgaggcgca gggcatcgag cgcctgcccg gtgttcacct ggggccgaac gtggtggaga
     4501 tggaaggccg gggcatccgc accgaccggg cagacgtggc cctgaacatc gacaccgcca
     4561 acgcccagat catcgactta caggaatacc gggaggcaat agaccatgaa cgcaatcgac
     4621 agagtgaaga aatccagagg catcaacgag ttagcggagc agatcgaacc gctggcccag
     4681 agcatggcga cactggccga cgaagcccgg caggtcatga gccagaccca gcaggccagc
     4741 gaggcgcagg cggcggagtg gctgaaagcc cagcgccaga caggggcggc atgggtggag
     4801 ctggccaaag agttgcggga ggtagccgcc gaggtgagca gcgccgcgca gagcgcccgg
     4861 agcgcgtcgc gggggtggca ctggaagcta tggctaaccg tgatgctggc ttccatgatg
     4921 cctacggtgg tgctgctgat cgcatcgttg ctcttgctcg acctgacgcc actgacaacc
     4981 gaggacggct cgatctggct gcgcttggtg gcccgatgaa gaacgacagg actttgcagg
     5041 ccataggccg acagctcaag gccatgggct gtgagcgctt cgatatcggc gtcagggacg
     5101 ccaccaccgg ccagatgatg aaccgggaat ggtcagccgc cgaagtgctc cagaacacgc
     5161 catggctcaa gcggatgaat gcccagggca atgacgtgta tatcaggccc gccgagcagg
     5221 agcggcatgg tctggtgctg gtggacgacc tcagcgagtt tgacctggat gacatgaaag
     5281 ccgagggccg ggagcctgcc ctggtagtgg aaaccagccc gaagaactat caggcatggg
     5341 tcaaggtggc cgacgccgca ggcggtgaac ttcgggggca gattgcccgg acgctggcca
     5401 gcgagtacga cgccgacccg gccagcgccg acagccgcca ctatggccgc ttggcgggct
     5461 tcaccaaccg caaggacaag cacaccaccc gcgccggtta tcagccgtgg gtgctgctgc
     5521 gtgaatccaa gggcaagacc gccaccgctg gcccggcgct ggtgcagcag gctggccagc
     5581 agatcgagca ggcccagcgg cagcaggaga aggcccgcag gctggccagc ctcgaactgc
     5641 ccgagcggca gcttagccgc caccggcgca cggcgctgga cgagtaccgc agcgagatgg
     5701 ccgggctggt caagcgcttc ggtgatgacc tcagcaagtg cgactttatc gccgcgcaga
     5761 agctggccag ccggggccgc agtgccgagg aaatcggcaa ggccatggcc gaggccagcc
     5821 cagcgctggc agagcgcaag cccggccacg aagcggatta catcgagcgc accgtcagca
     5881 aggtcatggg tctgcccagc gtccagcttg cgcgggccga gctggcacgg gcaccggcac
     5941 cccgccagcg aggcatggac aggggcgggc cagatttcag catgtagtgc ttgcgttggt
     6001 actcacgcct gttatactat gagtactcac gcacagaagg gggttttatg gaatacgaaa
     6061 aaagcgcttc agggtcggtc tacctgatca aaagtgacaa gggctattgg ttgcccggtg
     6121 gctttggtta tacgtcaaac aaggccgagg ctggccgctt ttcagtcgct gatatggcca
     6181 gccttaacct tgacggctgc accttgtcct tgttccgcga agacaagcct ttcggccccg
     6241 gcaagtttct cggtgactga tatgaaagac caaaaggaca agcagaccgg cgacctgctg
     6301 gccagccctg acgctgtacg ccaagcgcga tatgccgagc gcatgaaggc caaagggatg
     6361 cgtcagcgca agttctggct gaccgacgac gaatacgagg cgctgcgcga gtgcctggaa
     6421 gaactcagag cggcgcaggg cgggggtagt gaccccgcca gcgcctaacc accaactgcc
     6481 tgcaaaggag gcaatcaatg gctacccata agcctatcaa tattctggag gcgttcgcag
     6541 cagcgccgcc accgctggac tacgttttgc ccaacatggt ggccggtacg gtcggggcgc
     6601 tggtgtcgcc cggtggtgcc ggtaaatcca tgctggccct gcaactggcc gcacagattg
     6661 caggcgggcc ggatctgctg gaggtgggcg aactgcccac cggcccggtg atctacctgc
     6721 ccgccgaaga cccgcccacc gccattcatc accgcctgca cgcccttggg gcgcacctca
     6781 gcgccgagga acggcaagcc gtggctgacg gcctgctgat ccagccgctg atcggcagcc
     6841 tgcccaacat catggccccg gagtggttcg acggcctcaa gcgcgccgcc gagggccgcc
     6901 gcctgatggt gctggacacg ctgcgccggt tccacatcga ggaagaaaac gccagcggcc
     6961 ccatggccca ggtcatcggt cgcatggagg ccatcgccgc cgataccggg tgctctatcg
     7021 tgttcctgca ccatgccagc aagggcgcgg ccatgatggg cgcaggcgac cagcagcagg
     7081 ccagccgggg cagctcggta ctggtcgata acatccgctg gcagtcctac ctgtcgagca
     7141 tgaccagcgc cgaggccgag gaatggggtg tggacgacga ccagcgccgg ttcttcgtcc
     7201 gcttcggtgt gagcaaggcc aactatggcg caccgttcgc tgatcggtgg ttcaggcggc
     7261 atgacggcgg ggtgctcaag cccgccgtgc tggagaggca gcgcaagagc aagggggtgc
     7321 cccgtggtga agcctaagaa caagcacagc ctcagccacg tccggcacga cccggcgcac
     7381 tgtctggccc ccggcctgtt ccgtgccctc aagcggggcg agcgcaagcg cagcaagctg
     7441 gacgtgacgt atgactacgg cgacggcaag cggatcgagt tcagcggccc ggagccgctg
     7501 ggcgctgatg atctgcgcat cctgcaaggg ctggtggcca tggctgggcc taatggccta
     7561 gtgcttggcc cggaacccaa gaccgaaggc ggacggcagc tccggctgtt cctggaaccc
     7621 aagtgggagg ccgtcaccgc tgatgccatg gtggtcaaag gtagctatcg ggcgctggca
     7681 aaggaaatcg gggcagaggt cgatagtggt ggggcgctca agcacataca ggactgcatc
     7741 gagcgccttt ggaaggtatc catcatcgcc cagaatggcc gcaagcggca ggggtttcgg
     7801 ctgctgtcgg agtacgccag cgacgaggcg gacgggcgcc tgtacgtggc cctgaacccc
     7861 ttgatcgcgc aggccgtcat gggtggcggc cagcatgtgc gcatcagcat ggacgaggtg
     7921 cgggcgctgg acagcgaaac cgcccgcctg ctgcaccagc ggctgtgtgg ctggatcgac
     7981 cccggcaaaa ccggcaaggc ttccatagat accttgtgcg gctatgtctg gccgtcagag
     8041 gccagtggtt cgaccatgcg caagcgccgc cagcgggtgc gcgaggcgtt gccggagctg
     8101 gtcgcgctgg gctggacggt aaccgagttc gcggcgggca agtacgacat cacccggccc
     8161 aaggcggcag gctgaccccc cccactctat tgtaaacaag acatttttat cttttatatt
     8221 caatggctta ttttcctgct aattggtaat accatgaaaa ataccatgct cagaaaaggc
     8281 ttaacaatat tttgaaaaat tgcctactga gcgctgccgc acagctccat aggccgcttt
     8341 cctggctttg cttccagatg tatgctcttc tgctcccgaa cgccagcaag acgtagccca
     8401 gcgcgtcggc cagcttgcaa ttcgcgctaa cttacattaa ttgcgttgcg ctcactgccc
     8461 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8521 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     8581 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     8641 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     8701 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     8761 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     8821 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     8881 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     8941 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     9001 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     9061 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     9121 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     9181 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     9241 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     9301 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     9361 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     9421 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     9481 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     9541 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca
     9601 ttcgatggtg tcaacgtaaa tgccgcttcg ccttcgcgcg cgaattgcaa gctgatccgg
     9661 gcttatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg
     9721 tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt
     9781 ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct
     9841 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac
     9901 acaggaaaca gaattcgagc tcggtaccag gaggaaacga tgccacaatt tgatatatta
     9961 tgtaaaacac cacctaaggt gcttgttcgt cagtttgtgg aaaggtttga aagaccttca
    10021 ggtgagaaaa tagcattatg tgctgctgaa ctaacctatt tatgttggat gattacacat
    10081 aacggaacag caatcaagag agccacattc atgagctata atactatcat aagcaattcg
    10141 ctgagtttcg atattgtcaa taaatcactc cagtttaaat acaagacgca aaaagcaaca
    10201 attctggaag cctcattaaa gaaattgatt cctgcttggg aatttacaat tattccttac
    10261 tatggacaaa aacatcaatc tgatatcact gatattgtaa gtagtttgca attacagttc
    10321 gaatcatcgg aagaagcaga taagggaaat agccacagta aaaaaatgct taaagcactt
    10381 ctaagtgagg gtgaaagcat ctgggagatc actgagaaaa tactaaattc gtttgagtat
    10441 acttcgagat ttacaaaaac aaaaacttta taccaattcc tcttcctagc tactttcatc
    10501 aattgtggaa gattcagcga tattaagaac gttgatccga aatcatttaa attagtccaa
    10561 aataagtatc tgggagtaat aatccagtgt ttagtgacag agacaaagac aagcgttagt
    10621 aggcacatat acttctttag cgcaaggggt aggatcgatc cacttgtata tttggatgaa
    10681 tttttgagga attctgaacc agtcctaaaa cgagtaaata ggaccggcaa ttcttcaagc
    10741 aataaacagg aataccaatt attaaaagat aacttagtca gatcgtacaa taaagctttg
    10801 aagaaaaatg cgccttattc aatctttgct ataaaaaatg gcccaaaatc tcacattgga
    10861 agacatttga tgacctcatt tctttcaatg aagggcctaa cggagttgac taatgttgtg
    10921 ggaaattgga gcgataagcg tgcttctgcc gtggccagga caacgtatac tcatcagata
    10981 acagcaatac ctgatcacta cttcgcacta gtttctcggt actatgcata tgatccaata
    11041 tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg gcagcatata
    11101 gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa tgggataata
    11161 tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata agtcgacctg
    11221 caggcatgca
//