| 质粒名称: | pGEX-6p-1-hsG3BP2a |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000915 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-6p-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄青霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | G3BP2a |
| 物种来源: | human |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-6p-1-hsG3BP2a载体质粒基本信息
pGEX-6p-1-hsG3BP2a质粒图谱载体图谱和pGEX-6p-1-hsG3BP2a载体序列质粒序列多克隆位点信息
pGEX-6p-1-hsG3BP2a质粒载体简介
pGEX-6p-1-hsG3BP2a质粒序列载体序列
LOCUS pGEX-6p-1-hsG3BP2a 6399 bp DNA circular SYN 08-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6399)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6399
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQGS"
CDS 942..2390
/codon_start=1
/label=hsG3BP2A codon
/translation="MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGV
DASGKPQEAVYGQNDIHHKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQP
ERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPS
PEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEELE
EKSTTPPPAEPVSLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPPHVKAPVSQPRVEAK
PEVQSQPPRVREQRPRERPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPH
DIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSEPVQRILIAKPIMFRGE
VRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRGPPPRG
GMAQKLGSGRGTGQMEGRFTGQRR"
promoter 2702..2806
/gene="bla"
/label=AmpR promoter
CDS 2807..3667
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3838..4426
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4670..4747
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 4748..5830
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 5843..5864
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5879..5909
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5917..5933
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5941..5957
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5953..6399
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(5973..5989)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatgaa aacctgtatt ttcagggatc catggtgatg gagaagcctt
961 caccattact ggttggtcgt gagttcgtac gccagtatta tacgttactt aacaaagcac
1021 ccgagtattt acaccgcttt tacggacgca attcgtctta tgttcacggc ggcgtggatg
1081 ctagcgggaa gcctcaagag gcggtgtatg gccagaatga tattcatcat aaagttcttt
1141 ccttgaactt tagcgaatgt cacaccaaga ttcgtcacgt agacgctcat gctacattaa
1201 gtgatggggt tgtagtccaa gttatggggt tgctgtccaa cagtggtcaa cctgagcgca
1261 aattcatgca aacctttgtg cttgcacctg aaggtagcgt acctaataag ttctatgttc
1321 ataacgatat gttccgctac gaggatgaag tgttcggtga ctcagagcct gaattagacg
1381 aagagtctga agatgaagtg gaggaggaac aagaagagcg ccaaccctcc cccgagccgg
1441 ttcaggagaa cgccaatagc ggatactacg aagcccatcc cgtaacgaac ggaatcgagg
1501 aacccctgga agagagcagt catgagcctg agccagagcc ggaatcggaa acgaaaactg
1561 aagagttaaa accccaggtc gaagaaaaaa acttagaaga gctggaggaa aagtcaacga
1621 ccccgccccc ggctgagcca gtgagtttgc cgcaagagcc tcctaaagca ttcagctggg
1681 cctcagtaac ctccaagaat cttccgccat ccgggaccgt atcgtcgtct ggtattcctc
1741 ctcatgtcaa ggccccagtc tctcagccac gtgttgaagc aaagccagaa gtccagtcgc
1801 agcccccgcg tgtacgcgag caacgccctc gtgaacgtcc aggttttccc ccgcgtggtc
1861 cccgtcctgg acgtggagat atggagcaaa atgatagcga caaccgtcgc atcatccgct
1921 accccgacag ccaccaattg ttcgttggga atcttccaca cgatattgat gagaacgaac
1981 ttaaggagtt ttttatgagc ttcggtaatg tcgtggaact tcgtatcaac acgaagggcg
2041 tgggagggaa attgcccaac tttggatttg ttgtcttcga cgatagcgag ccggtccagc
2101 gtatcttaat cgcgaagcca atcatgtttc gcggggaggt gcgtttaaat gtagaggaaa
2161 aaaagacccg cgcggctcgc gagcgtgaga cccgtggagg aggtgatgat cgccgtgaca
2221 ttcgtcgtaa cgaccgcggg cccggaggac ctcgcggtat tgtaggggga gggatgatgc
2281 gtgaccgcga tggtcgcggt cctccaccac gcggagggat ggctcaaaaa ctgggatctg
2341 gccgcggcac cggtcaaatg gaaggacgtt tcacgggtca acgccgttaa gaattcatcg
2401 tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
2461 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
2521 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
2581 cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
2641 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
2701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
2761 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
2821 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
2881 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
2941 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
3001 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
3061 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
3121 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
3181 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
3241 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
3301 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
3361 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
3421 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
3481 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
3541 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
3601 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
3661 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
3721 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
3781 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
3841 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
3901 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
3961 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
4021 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
4081 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
4141 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
4201 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
4261 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
4321 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
4381 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
4441 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
4501 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
4561 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
4621 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg acaccatcga
4681 atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
4741 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
4801 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
4861 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
4921 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
4981 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
5041 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
5101 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
5161 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
5221 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
5281 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
5341 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
5401 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
5461 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
5521 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
5581 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
5641 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
5701 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
5761 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
5821 cgggcagtga gcgcaacgca attaatgtaa gttagctcac tcattaggca ccccaggctt
5881 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
5941 caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac
6001 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
6061 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
6121 ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag
6181 tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac
6241 gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc
6301 acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag
6361 gaaggccaga cgcgaattat ttttgatggc gttggaatt
//
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