ACTA2-P2A-GFP-PGK-Puro

LOCUS       ACTA2-P2A-GFP-PGK-Puro             8504 bp DNA     circular SYN 02-JUL-2026
DEFINITION  donor vector for targeting a 2A peptide followed by green 
            fluorescent reporter to the human ACTA2 locus at the end of the 
            endogenous coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    ACTA2-P2A-GFP-PGK-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Kwong G, Marquez HA, Yang C, Wong JY, Kotton DN
  TITLE     Generation of a Purified iPSC-Derived Smooth Muscle-like Population 
            for Cell Sheet Engineering.
  JOURNAL   Stem Cell Reports. 2019 Sep 10;13(3):499-514. doi: 
            10.1016/j.stemcr.2019.07.014. Epub 2019 Aug 15.
  PUBMED    31422908
REFERENCE   2  (bases 1 to 8504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2019.07.014"; journalName: "Stem Cell Reports"; 
            date: "2019-09-10- 10"; volume: "13"; issue: "3"; pages: "499-514"
FEATURES             Location/Qualifiers
     source          1..8504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    101..800
                     /label=HA-L
     CDS             801..818
                     /codon_start=1
                     /label=Linker
                     /translation="ASSGSG"
     CDS             819..875
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             876..1592
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(921..942)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1182..1201)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1529..1550
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1642..1659)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1648..1872
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(1895..1928)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        1989..2487
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2024..2047)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2420..2439
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2527..3126
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2527..2546)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    complement(3683..3716)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3749..4351
                     /label=HA-R
     primer_bind     complement(4733..4752)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4734..4752)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4759..4776)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4759..4775)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4768..4790)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4916..5344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5003..5022)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5213..5234
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5688..6482
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5742..5761)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6352..6371
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             6500..7360
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6718..6737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7531..8119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8020..8039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8273..8290
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8407..8428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8443..8473
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8481..8497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8486..8504,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttggtac cgagctcgga tccactagta
       61 acggccgcca gtgtgctgga attcgccctt ttcctgcagg aggccaacca gtgcagctta
      121 agaggcacct attgcaaggg aatgtcccca gcaggggacc ctgagttccc caatgttaaa
      181 cagagcatct gggtgaaaag tggtagttca cttcaccacg tggaaacaca tttgtatact
      241 aagtcctagc acaattaaaa cttctccttt gaataggcta gggtttgttt ctacagaaag
      301 tgtatcgtgt tgaattctga ggcagagttc atggaaatca gaggcattgc cagggttctt
      361 aagtgggggc catggctgga tttggagcca actttcagtt tgctcaataa tgtggtcgaa
      421 gagttacagg tgagaaaaac gaatgtgctt actgaagaat ttctaaatcc atggctccct
      481 ctggcggtcc tgccaagaac atgacacaat taggacacaa ttggcatcta acagacaagc
      541 ctgtgtccct gctgaacaag aaactaggcc atggtagaac atccaggctc tgtcctaccg
      601 cactaacgac tgcactaatg atgacattaa tgaccattct ttttcatctt gactgacaga
      661 tcattgcccc tccggagcgc aaatactctg tctggatcgg tggctccatc ctggcctctc
      721 tgtccacctt ccagcagatg tggatcagca aacaggaata cgatgaagcc gggccttcca
      781 ttgtgcaccg caaatgcttc gctagctctg gcagcggagc cactaacttc tccctgttga
      841 aacaagcagg ggatgtcgaa gagaatcccg ggccaatggt gagcaagggc gaggagctgt
      901 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      961 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1021 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1081 tgcagtgctt cagtcgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1141 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1201 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1261 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1321 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1381 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1441 tcggcgacgg ccccgtgctg ctgcctgaca accactacct gagcacccag tccgccctga
     1501 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1561 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgtcgag tctagagggc
     1621 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     1681 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1741 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1801 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     1861 tgggctctat ggcttctgag gcggaaagaa ccagataact tcgtataatg tatgctatac
     1921 gaagttatta ggtctgaaga ggagtttacg tccagccaag cttaggatct cgacctcgaa
     1981 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     2041 cccgctggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2101 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2161 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2221 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2281 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2341 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2401 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     2461 tcctcttcct catctccggg cctttcgacc tgcatccatc tagatctcga gcagctgaag
     2521 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     2581 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     2641 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     2701 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     2761 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     2821 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2881 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     2941 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     3001 ttcctggaga cctccgcgcc ccacaacctc cccttctacg agcggctcgg cttcaccgtc
     3061 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     3121 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     3181 tcgatgatat cagatccccg ggatgcagaa attgatgatc tattaaacaa taaagatgtc
     3241 cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta
     3301 cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc
     3361 ctgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc
     3421 ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat
     3481 agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt tgtggttcta
     3541 agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc agcagcctct
     3601 gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca tcagaagctg
     3661 gtcgagatcc ggaaccctta atataacttc gtataatgta tgctatacga agttattagg
     3721 tccctcgaag aggttcacta ggcgcgccac ttcttgcgtt ggcctccgat caatactcga
     3781 ctgataccat ttgttaggtt gacctcaccc gttacaggat ggctccctct agtggtccag
     3841 ctgagcacat ggcaattagg atggatatgt ttctcaggca aagggtagaa tcacattacc
     3901 tgaagtgggg agctgcactt cggttctgta aagaggaata atttgagaat acattggaga
     3961 ttttaaaaaa taacatggat taaagtgtat caatgggata atctacagta cctcacaact
     4021 tgcaaagtga ttatattact ttacactcat aaccccagta ctggtaacct gatgggtgaa
     4081 aagaaaataa ttacacacac acacacacgt atatatacac atatacacac atatatatgt
     4141 atgtgtgtgt gtacatatat atatgtatgt gtgtatatat atatatgtgt gtgtgtgtgt
     4201 gtgtgtgtgt gtattagaga taaggtctca ctctgtcacc caggctggag tgcaatgatg
     4261 taataatagc tcattgcagc cttgatgcag ccttgaactt ctaggctcaa gcaattctct
     4321 cctcttgact ttccaaaatg ctggaattat aggcataagc cgctgtgcct atatatctta
     4381 aaagtcatat cttgtatatt aaaagtcaca cacacacata tagatacatt tcccatacat
     4441 atatacatat atatgtgtgt gacttttaat atacaagata tgacttttaa tgcatatact
     4501 atgtattaaa cttatataca tatatataca catacatata tacatataga aacaggtggt
     4561 ctgactttta tgtttattga gggaaacatg gattttttaa aaaacattga gaaacactgc
     4621 cttaaaatct cactacacat ctggaccatc tgtggccggc caaaagggcg aattctgcag
     4681 atatccatca cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag
     4741 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4801 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4861 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
     4921 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4981 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5041 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5101 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5161 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5221 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5281 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5341 aattttaaca aaattcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
     5401 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     5461 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     5521 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     5581 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     5641 gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5701 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5761 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5821 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5881 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5941 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6001 cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6061 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6121 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6181 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6241 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6301 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6361 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6421 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6481 gaattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6601 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6781 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6841 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6901 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6961 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7021 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7081 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7141 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7201 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7261 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7321 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7381 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7441 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt caca
//