| 质粒名称: | pLYL176 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002790 |
| 产品用途: | Mammalian constitutive expression vector for Type-C TMP-inducible EPO secretion (PhCMV-EPO-FCS-ecDHFRN18T, A19V-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ecDHFRN18T, A19V/EPO |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pLYL176
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL176载体质粒基本信息
pLYL176质粒图谱载体图谱和pLYL176载体序列质粒序列多克隆位点信息
pLYL176质粒载体简介
pLYL176质粒序列载体序列
LOCUS pLYL176 6452 bp DNA circular SYN 15-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6452)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6452
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 213..241
/label=OGT82-1-F
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..908
/label=olyl401
primer_bind 897..922
/label=olyl479
CDS 923..1501
/codon_start=1
/gene="EPO"
/gene_synonym="DBAL; ECYT5; EP; MVCD2"
/product="erythropoietin precursor"
/label=EPO
/note="epoetin"
/db_xref="CCDS:CCDS5705.1"
/db_xref="GeneID:2056"
/db_xref="HGNC:HGNC:3415"
/db_xref="MIM:133170"
/protein_id="NP_000790.2"
/translation="MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICDSRVLERYLLE
AKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRG
QALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITAD
TFRKLFRVYSNFLRGKLKLYTGEACRTGDR"
sig_peptide 923..1003
/gene="EPO"
/gene_synonym="DBAL; ECYT5; EP; MVCD2"
/inference="COORDINATES:same species:SignalP"
misc_feature 1073..1075
/gene="EPO"
/gene_synonym="DBAL; ECYT5; EP; MVCD2"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000269|PubMed:3949763. /id=CAR_000052;
propagated from UniProtKB/Swiss-Prot (P01588.1);
glycosylation site"
misc_feature 1115..1117
/gene="EPO"
/gene_synonym="DBAL; ECYT5; EP; MVCD2"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000269|PubMed:3949763. /id=CAR_000166;
propagated from UniProtKB/Swiss-Prot (P01588.1);
glycosylation site"
misc_feature 1250..1252
/gene="EPO"
/gene_synonym="DBAL; ECYT5; EP; MVCD2"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000269|PubMed:3949763. /id=CAR_000192;
propagated from UniProtKB/Swiss-Prot (P01588.1);
glycosylation site"
misc_feature 1379..1381
/gene="EPO"
/gene_synonym="DBAL; ECYT5; EP; MVCD2"
/note="O-linked (GalNAc...) serine.
/evidence=ECO:0000269|PubMed:3949763; propagated from
UniProtKB/Swiss-Prot (P01588.1); glycosylation site"
primer_bind 1502..1550
/label=olyl345
CDS 1502..1513
/codon_start=1
/label=fcs
/translation="RIKR"
primer_bind 1509..1550
/label=olyl339
misc_feature 1514..1528
/label=GS Linker
primer_bind complement(1524..1548)
/label=olyl484
primer_bind 1525..1550
/label=olyl347
misc_feature 1535..2013
/label=ecDHFR(N18T/A19V)
primer_bind complement(1989..2011)
/label=olyl402
primer_bind complement(2007..2034)
/label=olyl340
terminator 2049..2276
/label=bGH PA
misc_feature 2944..3021
/label=SV40 ORI
CDS 3163..3954
/label=Kan/neoR
primer_bind complement(4294..4314)
/label=M13-rev
misc_binding complement(4320..4342)
/label=LacO
promoter complement(4347..4376)
/label=lac
CDS complement(5459..6118)
/label=AmpR
promoter complement(6358..6386)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgggggt gcacgaatgt cctgcctggc tgtggcttct
961 cctgtccctg ctgtcgctcc ctctgggcct cccagtcctg ggcgccccac cacgcctcat
1021 ctgtgacagc cgagtcctgg agaggtatct cttggaggcc aaggaggctg agaatatcac
1081 gacgggctgt gctgaacact gcagcttgaa tgagaatatc actgtcccag acaccaaagt
1141 taatttctat gcctggaaga ggatggaggt cgggcagcag gccgtagaag tctggcaggg
1201 cctggccctg ctgtcggaag ctgtcctgcg gggccaggcc ctgttggtca actcttccca
1261 gccgtgggag cccctgcagc tgcatgtgga taaagccgtc agtggccttc gcagcctcac
1321 cactctgctt cgggctctgg gagcccagaa ggaagccatc tcccctccag atgcggcctc
1381 agctgctcca ctccgaacaa tcactgctga cactttccgc aaactcttcc gagtctactc
1441 caatttcctc cggggaaagc tgaagctgta cacaggggag gcctgtagga caggggacag
1501 aaggatcaag aggggaggag gatctggaga attcatgatc agcctgatcg ccgccctggc
1561 cgtggaccac gtgatcggca tggagaccgt gatgccctgg aacctgcccg ccgacctggc
1621 ctggttcaag agaaacaccc tgaacaagcc cgtgatcatg ggcagacaca cctgggagag
1681 catcggcaga cccctgcccg gcagaaagaa catcatcctg agcagccagc ccagcaccga
1741 cgacagagtg acctgggtga agagcgtgga cgaggccatc gccgcctgcg gcgacgtgcc
1801 cgagatcatg gtgatcggcg gcggcagagt gtacgagcag ttcctgccca aggcccagaa
1861 gctgtacctg acccacatcg acgccgaggt ggagggcgac acccacttcc ccgactacga
1921 gcccgacgac tgggagagcg tgttcagcga gttccacgac gccgacgccc agaacagcca
1981 cagctactgc ttcgagatcc tggagagaag atagtctaga gggcccgttt aaacccgctg
2041 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
2101 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
2161 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
2221 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
2281 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
2341 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
2401 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
2461 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
2521 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
2581 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
2641 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
2701 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
2761 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2821 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
2881 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
2941 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
3001 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
3061 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
3121 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
3181 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
3241 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
3301 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
3361 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
3421 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
3481 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
3541 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
3601 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
3661 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
3721 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
3781 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
3841 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
3901 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
3961 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
4021 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
4081 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
4141 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
4201 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
4261 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
4321 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
4381 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
4441 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
4501 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
4561 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
4621 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
4681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
4741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
4801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
4861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
4921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
4981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
5041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
5101 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
5161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
5221 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
5281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
5341 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
5401 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
5461 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
5521 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
5581 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
5641 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
5701 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
5761 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
5821 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
5881 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
5941 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
6001 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
6061 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
6121 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
6181 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
6241 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
6301 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
6361 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
6421 gcacatttcc ccgaaaagtg ccacctgacg tc
//
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