| 质粒名称: | pEB1deltaC-mCherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 80603 |
| 质粒用途: | Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end |
| 存储实验室: | Torsten Wittmann |
|
载体骨架基本信息 |
|
| 载体名称: | pmCherry-N1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | 5400 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | EB1，MAPRE1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 747 bp |
| 基因突变: | Truncated C-terminus; EB1 coding sequence ends at threonine 249 |
| GenBank编号 | NM_012325 |
| 启动子: | CMV |
| 标签/融合蛋白: | mCherry (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Nhe1（未销毁） |
| 3’克隆位点: | Not1（未销毁） |
| 5’测序引物 | CMV forward |
| 3’测序引物 | mCherry reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pEB1deltaC-mCherry was a gift from Torsten Wittmann (Addgene plasmid # 80603 ; http://n2t.net/addgene:80603 ; RRID:Addgene_80603) |
| 参考文献部分: | Doublecortin Is Excluded from Growing Microtubule Ends and Recognizes the GDP-Microtubule Lattice. Ettinger A, van Haren J, Ribeiro SA, Wittmann T. Curr Biol. 2016 Jun 20;26(12):1549-55. doi: 10.1016/j.cub.2016.04.020. Epub 2016 May 26. 10.1016/j.cub.2016.04.020 PubMed 27238282 |
pEB1deltaC-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pEB1deltaC-mCherry载体质粒基本信息
pEB1deltaC-mCherry质粒图谱载体图谱和pEB1deltaC-mCherry载体序列质粒序列多克隆位点信息
pEB1deltaC-mCherry质粒载体简介
pEB1deltaC-mCherry质粒序列载体序列
LOCUS pEB1deltaC-mCherry 5438 bp DNA circular SYN 04-SEP-2026
DEFINITION Expresses C-terminally truncated, mCherry-tagged EB1 as marker for
growing microtubule plus end.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5438)
AUTHORS Ettinger A, van Haren J, Ribeiro SA, Wittmann T
TITLE Doublecortin Is Excluded from Growing Microtubule Ends and
Recognizes the GDP-Microtubule Lattice.
JOURNAL Curr Biol. 2016 Jun 20;26(12):1549-55. doi:
10.1016/j.cub.2016.04.020. Epub 2016 May 26.
PUBMED 27238282
REFERENCE 2 (bases 1 to 5438)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 4, 2026 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cub.2016.04.020"; journalName: "Curr Biol"; date:
"2016-06-20- 20"; volume: "26"; issue: "12"; pages: "1549-55"
COMMENT Sequence Label: pEB1deltaC-mCherry
FEATURES Location/Qualifiers
source 1..5438
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 1389..2096
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(1534..1552)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1802..1821
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2226..2347
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2263..2282)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2317..2336
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2354..2809)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2491..2512)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2703..2722
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2836..2940
/gene="bla"
/label=AmpR promoter
promoter 2942..3299
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2942..3133
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2965..2985)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3150..3285
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3334..4128
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3334..4128
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3388..3407)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3998..4017
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4316..4335)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4360..4407
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4736..5324
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5225..5244
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca
601 tggcagtgaa cgtatactca acgtcagtga ccagtgataa cctaagtcga catgacatgc
661 tggcctggat caatgagtct ctgcagttga atctgacaaa gatcgaacag ttgtgctcag
721 gggctgcgta ttgtcagttt atggacatgc tgttccctgg ctccattgcc ttgaagaaag
781 tgaaattcca agctaagcta gaacacgagt acatccagaa cttcaaaata ttacaagcag
841 gttttaagag aatgggtgtt gacaaaataa ttcctgtgga caaattagta aaaggaaagt
901 ttcaggacaa ttttgaattc gttcagtggt tcaagaagtt cttcgatgca aactatgatg
961 gaaaagacta tgaccctgtg gctgctagac aaggtcaaga aactgcagtg gctccttccc
1021 ttgttgctcc agctctgaat aaaccgaaga aacctctcac ttctagcagt gcagctcccc
1081 agaggcccat ctcaacacag agaaccgctg cggctcctaa ggctggccct ggtgtggtgc
1141 gaaagaaccc tggtgtgggc aacggagacg acgaggcagc tgagttgatg cagcaggtca
1201 acgtattgaa acttactgtt gaagacttgg agaaagagag ggatttctac ttcggaaagc
1261 tacggaacat tgaattgatt tgccaggaga acgaggggga aaacgaccct gtattgcaga
1321 ggattgtaga cattctgtat gccacccggg acatggtgag caagggccaa gaagagtata
1381 cccgggacat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
1441 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
1501 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
1561 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
1621 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
1681 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
1741 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
1801 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
1861 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
1921 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
1981 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
2041 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat
2101 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
2161 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
2221 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
2281 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
2341 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
2401 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
2461 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
2521 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
2581 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
2641 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
2701 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
2761 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
2821 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
2881 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
2941 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
3001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
3061 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
3121 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
3181 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
3241 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
3301 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
3361 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
3421 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
3481 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
3541 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
3601 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
3661 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
3721 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
3781 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
3841 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
3901 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
3961 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
4021 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
4081 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
4141 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
4201 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
4261 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
4321 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
4381 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
4441 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
4501 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
4561 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
4621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
4681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
4741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
4801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
4861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
4921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
4981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
5041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
5101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
5161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
5221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
5281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
5341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
5401 cccctgattc tgtggataac cgtattaccg ccatgcat
//
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