| 质粒名称: | pX601-GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 84040 |
| 存储实验室: | Yuet Wai Kan |
|
载体骨架基本信息 |
|
| 载体名称: | pX601 |
| 载体出品公司: | Feng Zhang's Laboratory |
| 空载体大小: | 7447 bp |
| 完整质粒大小: | 8138 bp |
| 载体修饰: | 将GFP插入SaCas9的下游 |
| 载体类型: | 哺乳细胞表达,AAV |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | 淋病奈瑟球菌 |
| GenBank编号 | 7011691 |
| 标签/融合蛋白: | GFP（插入件上的C端子） |
| 5’测序引物 | Cas9-HindIII 5' -S:gccccgtcgtgaagagaagcttcatcc |
| 3’测序引物 | EGFP-EcoR1-AS:cgtagaattcggccgctttacttgtacagctcgtccatgccga |
|
文献引用方法 |
|
| 材料和方法部分: | pX601-GFP was a gift from Yuet Wai Kan (Addgene plasmid # 84040 ; http://n2t.net/addgene:84040 ; RRID:Addgene_84040) |
| 参考文献部分: | Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia. Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW. Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6. 10.1073/pnas.1612075113 PubMed 27601644 |
买家导航
pX601-GFP载体 pX601GFP质粒基本信息
pX601-GFP质粒图谱 pX601GFP载体图谱和pX601-GFP载体序列 pX601GFP质粒序列多克隆位点信息
pX601-GFP质粒 pX601GFP载体简介
pX601-GFP质粒序列 pX601GFP载体序列
LOCUS pX601-GFP 8138 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Staphylococcus aureus (SaCas9) conjugated with GFP.
ACCESSION .
VERSION .
KEYWORDS pX601-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8138)
AUTHORS Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW
TITLE Genome editing using CRISPR-Cas9 to create the HPFH genotype in
HSPCs: An approach for treating sickle cell disease and
beta-thalassemia.
JOURNAL Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi:
10.1073/pnas.1612075113. Epub 2016 Sep 6.
PUBMED 27601644
REFERENCE 2 (bases 1 to 8138)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8138
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
enhancer 154..533
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 534..737
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 688..708
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 756..765
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 768..788
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 813..3968
/codon_start=1
/product="Cas9 endonuclease from the Staphylococcus aureus
Type II CRISPR/Cas system"
/label=SaCas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
IKKG"
CDS 3969..4016
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 4017..4070
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 4071..4790
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 4071..4787
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4116..4137)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4377..4396)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4724..4745
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4822..4839)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4828..5035
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 5043..5283
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 5043..5063
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 5214..5233
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 5312..5387
/label=Sa gRNA scaffold
/note="guide RNA scaffold for the Staphylococcus aureus
CRISPR/Cas9 system"
repeat_region 5401..5541
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5401..5530
/label=AAV2 ITR
rep_origin 5616..6071
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5703..5722)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5913..5934
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6088..6107)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6207..6229
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6267..6285)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6353..6457
/gene="bla"
/label=AmpR promoter
CDS 6458..7318
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6676..6695)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7489..8077
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7978..7997
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
721 tctatataag cagagctctc tggctaacta ccggtgccac catggcccca aagaagaagc
781 ggaaggtcgg tatccacgga gtcccagcag ccaagcggaa ctacatcctg ggcctggaca
841 tcggcatcac cagcgtgggc tacggcatca tcgactacga gacacgggac gtgatcgatg
901 ccggcgtgcg gctgttcaaa gaggccaacg tggaaaacaa cgagggcagg cggagcaaga
961 gaggcgccag aaggctgaag cggcggaggc ggcatagaat ccagagagtg aagaagctgc
1021 tgttcgacta caacctgctg accgaccaca gcgagctgag cggcatcaac ccctacgagg
1081 ccagagtgaa gggcctgagc cagaagctga gcgaggaaga gttctctgcc gccctgctgc
1141 acctggccaa gagaagaggc gtgcacaacg tgaacgaggt ggaagaggac accggcaacg
1201 agctgtccac caaagagcag atcagccgga acagcaaggc cctggaagag aaatacgtgg
1261 ccgaactgca gctggaacgg ctgaagaaag acggcgaagt gcggggcagc atcaacagat
1321 tcaagaccag cgactacgtg aaagaagcca aacagctgct gaaggtgcag aaggcctacc
1381 accagctgga ccagagcttc atcgacacct acatcgacct gctggaaacc cggcggacct
1441 actatgaggg acctggcgag ggcagcccct tcggctggaa ggacatcaaa gaatggtacg
1501 agatgctgat gggccactgc acctacttcc ccgaggaact gcggagcgtg aagtacgcct
1561 acaacgccga cctgtacaac gccctgaacg acctgaacaa tctcgtgatc accagggacg
1621 agaacgagaa gctggaatat tacgagaagt tccagatcat cgagaacgtg ttcaagcaga
1681 agaagaagcc caccctgaag cagatcgcca aagaaatcct cgtgaacgaa gaggatatta
1741 agggctacag agtgaccagc accggcaagc ccgagttcac caacctgaag gtgtaccacg
1801 acatcaagga cattaccgcc cggaaagaga ttattgagaa cgccgagctg ctggatcaga
1861 ttgccaagat cctgaccatc taccagagca gcgaggacat ccaggaagaa ctgaccaatc
1921 tgaactccga gctgacccag gaagagatcg agcagatctc taatctgaag ggctataccg
1981 gcacccacaa cctgagcctg aaggccatca acctgatcct ggacgagctg tggcacacca
2041 acgacaacca gatcgctatc ttcaaccggc tgaagctggt gcccaagaag gtggacctgt
2101 cccagcagaa agagatcccc accaccctgg tggacgactt catcctgagc cccgtcgtga
2161 agagaagctt catccagagc atcaaagtga tcaacgccat catcaagaag tacggcctgc
2221 ccaacgacat cattatcgag ctggcccgcg agaagaactc caaggacgcc cagaaaatga
2281 tcaacgagat gcagaagcgg aaccggcaga ccaacgagcg gatcgaggaa atcatccgga
2341 ccaccggcaa agagaacgcc aagtacctga tcgagaagat caagctgcac gacatgcagg
2401 aaggcaagtg cctgtacagc ctggaagcca tccctctgga agatctgctg aacaacccct
2461 tcaactatga ggtggaccac atcatcccca gaagcgtgtc cttcgacaac agcttcaaca
2521 acaaggtgct cgtgaagcag gaagaaaaca gcaagaaggg caaccggacc ccattccagt
2581 acctgagcag cagcgacagc aagatcagct acgaaacctt caagaagcac atcctgaatc
2641 tggccaaggg caagggcaga atcagcaaga ccaagaaaga gtatctgctg gaagaacggg
2701 acatcaacag gttctccgtg cagaaagact tcatcaaccg gaacctggtg gataccagat
2761 acgccaccag aggcctgatg aacctgctgc ggagctactt cagagtgaac aacctggacg
2821 tgaaagtgaa gtccatcaat ggcggcttca ccagctttct gcggcggaag tggaagttta
2881 agaaagagcg gaacaagggg tacaagcacc acgccgagga cgccctgatc attgccaacg
2941 ccgatttcat cttcaaagag tggaagaaac tggacaaggc caaaaaagtg atggaaaacc
3001 agatgttcga ggaaaagcag gccgagagca tgcccgagat cgaaaccgag caggagtaca
3061 aagagatctt catcaccccc caccagatca agcacattaa ggacttcaag gactacaagt
3121 acagccaccg ggtggacaag aagcctaata gagagctgat taacgacacc ctgtactcca
3181 cccggaagga cgacaagggc aacaccctga tcgtgaacaa tctgaacggc ctgtacgaca
3241 aggacaatga caagctgaaa aagctgatca acaagagccc cgaaaagctg ctgatgtacc
3301 accacgaccc ccagacctac cagaaactga agctgattat ggaacagtac ggcgacgaga
3361 agaatcccct gtacaagtac tacgaggaaa ccgggaacta cctgaccaag tactccaaaa
3421 aggacaacgg ccccgtgatc aagaagatta agtattacgg caacaaactg aacgcccatc
3481 tggacatcac cgacgactac cccaacagca gaaacaaggt cgtgaagctg tccctgaagc
3541 cctacagatt cgacgtgtac ctggacaatg gcgtgtacaa gttcgtgacc gtgaagaatc
3601 tggatgtgat caaaaaagaa aactactacg aagtgaatag caagtgctat gaggaagcta
3661 agaagctgaa gaagatcagc aaccaggccg agtttatcgc ctccttctac aacaacgatc
3721 tgatcaagat caacggcgag ctgtatagag tgatcggcgt gaacaacgac ctgctgaacc
3781 ggatcgaagt gaacatgatc gacatcacct accgcgagta cctggaaaac atgaacgaca
3841 agaggccccc caggatcatt aagacaatcg cctccaagac ccagagcatt aagaagtaca
3901 gcacagacat tctgggcaac ctgtatgaag tgaaatctaa gaagcaccct cagatcatca
3961 aaaagggcaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggagg
4021 gcagaggatc cctgctaaca tgtggtgacg tcgaggagaa tcctggccca atggtgagca
4081 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
4141 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
4201 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
4261 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
4321 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
4381 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
4441 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
4501 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
4561 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
4621 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
4681 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
4741 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgaa
4801 ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
4861 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
4921 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
4981 tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct ggggaggtac
5041 ctgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
5101 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
5161 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
5221 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
5281 gacgaaacac cggagaccac ggcaggtctc agttttagta ctctggaaac agaatctact
5341 aaaacaaggc aaaatgccgt gtttatctcg tcaacttgtt ggcgagattt ttgcggccgc
5401 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
5461 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
5521 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
5581 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
5641 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
5701 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
5761 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
5821 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
5881 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
5941 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
6001 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
6061 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
6121 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
6181 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
6241 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
6301 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
6361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
6421 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
6481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
6541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
6601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
6661 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
6721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
6781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
6841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
6901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
6961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
7021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
7081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
7141 tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg
7201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
7261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
7321 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
7381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
7441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
7501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
7561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
7621 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
7681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
7741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
7801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
7861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
7921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
7981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
8041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
8101 acggttcctg gccttttgct ggccttttgc tcacatgt
//
