| 质粒名称: | pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000250 |
| 产品用途: | Expresses Nier1s and EGFP in mammalian cells culture |
| 存储实验室: | Peng Zou Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Nier1s, EGFP |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators
Lin C., Liu L., Zou P.
Cell Reports Methods 3, 100544 (2023)
|
pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS载体质粒基本信息
pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS质粒图谱载体图谱和pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS载体序列质粒序列多克隆位点信息
pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS质粒载体简介
pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS质粒序列载体序列
LOCUS pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS 7842 bp DNA circular SYN 31-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7842)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7842
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 232..819
/label=CMV promoter
primer_bind 769..789
/label=CMV forward primer
misc_feature 804..807
/label=TATA box
misc_feature 830
/label=putative transcriptional start
primer_bind 863..882
/label=T7 primer
promoter 863..879
/label=T7 promoter
misc_feature 960..968
/label=Feature 13
misc_feature 963..968
/label=Kozak sequence
misc_feature 969..2453
/label=Nier1s
misc_structure 969..1478
/label=mIFP1-170
misc_structure 1488..1928
/label=Calmodulin
misc_structure 1944..2012
/label=RS20
misc_structure 2025..2453
/label=mIFP178-320
CDS 2460..2480
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2484..2504
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2508..2528
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 2529..2594
/label=P2A
/note="Geneious type: motif"
primer_bind 2529..2547
misc_feature 2581..2595
/label=Gibson
primer_bind 2585..2600
misc_feature join(2601..2795,2805..3317)
/label=eGFP
/label=eGFP(1)
misc_feature 2796..2804
/label=eGFP
misc_feature 2796..2804
/label=eGFP Chromophore
CDS 3324..3344
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3348..3368
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3372..3392
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 3393..3395
/label=Stop
misc_feature 3413..3418
/label=AflII
primer_bind 3436..3453
/label=BGH reverse primer
polyA_site 3442..3666
/label=BGH pA
rep_origin 3712..4140
/label=f1 origin
promoter 4145..4514
/label=SV40 early promoter
CDS 4550..5344
/codon_start=1
/label=Neo
/label=Neo(R)
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5518..5648
/label=SV40 pA
rep_origin complement(6031..6701)
/direction=LEFT
/label=pUC origin
CDS complement(6846..7706)
/codon_start=1
/label=Amp
/label=Amp(R)
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7707..7805)
/label=bla promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggggcaaaga attggggttt
901 gtctggtcaa ccaccgcggt ctccgtcgtc aggatcatcc gcagattata ccgcaactac
961 acgccaccat gtcggtaccg ctgactacct cagcattcgg ccacgcgttt ctggctaact
1021 gtgaacgtga gcagatccac ctggcgggct ccattcagcc gcacggtatc ctgctggctg
1081 ttaaagagcc tgacaacgtg gtgatccagg cttctattaa cgctgcggag ttcctgaaca
1141 ccaactttgt tgttggccgt ccgctgcgtg acctgggcgg cgatctgcct ttgcagatcc
1201 tgccgcacct gaacggcccg ctgcacctgg ctccgatgac cctgcgttgt actgtgggtt
1261 ctccgccgcg tcgtgtggac tgtaccattc atcgaccgtc taacggcggc ctgatcgtag
1321 aactggaacc agcaaccaag gccactaaca ttgcgccggc tctgcagggt gcgcttcatc
1381 gtatcacttc ttcatcctcc ctgatgggcc tgtgtgacga aaccgcgact attttccgtg
1441 agattactgg cttcgaccgt gtgatggtaa tgcgtctcgg cgcgcttgac gatctgactg
1501 aagagcagat cgcagagatt aaagaggctt tctccctatt tgacaaggac ggggacggga
1561 cgataacaac caaggagctg gggacggtgt tccggtctct ggggcagaac cccacagaag
1621 cagagctgca ggacatgatc aatgaagtag atgccgatgg cgacggcatc ttcgacttcc
1681 ctgagttcct gacgatgatg gcaaggaaaa tgaatgactc agacagtgaa gaggaaatta
1741 gaggcgcgtt ccgcgtgttt gataaggacg gcaatggcta catcggcgca gcagagcttc
1801 gccacgtgat gacagacctt ggtgagaagt taactgatga ggaggttgat gaaatgatca
1861 gggtagcaga caaggatggg gatggtcagg taaactacga agagtttgta caaatgatga
1921 cagcgaaggg tggcggaggt tctgtagatt catcacgtcg taagtggaat aaggcaggtc
1981 acgcagtcag agctataggt cggctgggct cgcgtaggca tgatttgctg tccgaatgtc
2041 gtcgtgcgga cctggaagcg ttcctgggta accgctaccc ggcgtctact attccgcaga
2101 tcgctcgtcg cctgtacgaa cttaaccgtg ttcgcctgct ggtagatgtg aactatactc
2161 cggttccgct agagccgcgc atcagcccgc tgaacggtcg tgatctggat atgtccctgt
2221 cttgcctgcg ctctatgtcc ccgatccacc agaaatacat gcaggacatg ggcgttggcg
2281 caaccctggt ttgctctctg atggtgtctg gtcgtctgtg gggtctgatc gtttgccacc
2341 actacgaacc gcgctacgtt ccgatgcaca ttcgcgctgc tggcgaagcg ctggcggaag
2401 cctgtgcgaa ccgcatcgcg acgctggaga gctttgcaca gtctcagtcc aaaaccaggc
2461 cgaaaaagaa gcgcaaggtt gaccctaaga agaagcgtaa agtcgaccca aagaagaaac
2521 ggaaggtggg ctctggagcc accaacttca gcctgctgaa gcaggcaggc gacgtggaag
2581 agaaccctgg ccctggatcc atggtgagca agggcgagga gctgttcacc ggggtggtgc
2641 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
2701 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
2761 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
2821 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
2881 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
2941 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
3001 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
3061 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
3121 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
3181 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
3241 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
3301 tggacgagct gtacaagacg cgtccaaaaa agaagagaaa ggtagacccg aagaaaaagc
3361 gtaaggtcga tcccaaaaag aaacggaaag tgtaagttta aatggtctcc agcttaagtt
3421 taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
3481 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
3541 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
3601 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
3661 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
3721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
3961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
4081 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4141 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
4201 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
4261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
4321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
4381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
4441 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
4501 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
4561 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
4621 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
4681 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
4741 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
4801 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
4861 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
4921 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
4981 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
5041 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
5101 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
5161 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
5221 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
5281 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
5341 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
5401 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
5461 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
5521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
5581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
5641 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
5701 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
5761 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
5821 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
5881 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
5941 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
6001 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
6061 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
6121 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
6181 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
6241 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
6301 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
6361 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
6421 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
6481 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
6541 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
6601 ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga
6661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
6721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
6781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
6841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
6901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
6961 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
7021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
7081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
7141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
7201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
7261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
7321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
7381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
7441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
7501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
7561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
7621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
7681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
7741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
7801 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
