| 质粒名称: | mCherry-Rab7a-7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 55127 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mCherry |
| 载体出品公司: | Clontech |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | 5307bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rab7a、NM_004637.5 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 951 bp |
| 基因突变: | N117S in NM_004637.5 |
| 启动子: | CMV |
| 标签/融合蛋白: | mCherry (N terminal on backbone) |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mCherry-Rab7a-7 was a gift from Michael Davidson (Addgene plasmid # 55127 ; http://n2t.net/addgene:55127 ; RRID:Addgene_55127) |
买家导航
mCherry-Rab7a-7载体质粒基本信息
mCherry-Rab7a-7质粒图谱载体图谱和mCherry-Rab7a-7载体序列质粒序列多克隆位点信息
mCherry-Rab7a-7质粒载体简介
mCherry-Rab7a-7质粒序列载体序列
LOCUS mCherry-Rab7a-7 5307 bp DNA circular SYN 14-APR-2025
DEFINITION Localization: Ras GTPase, Excitation: 587, Emission: 610.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5307)
TITLE Davidson lab - mCherry constructs
REFERENCE 2 (bases 1 to 5307)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Sequence Label: mCherry-Rab7a-7
FEATURES Location/Qualifiers
source 1..5307
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 137..440
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 441..644
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 595..615
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 683..692
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 689..1396
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(834..852)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1102..1121
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2171..2292
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2208..2227)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2262..2281
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2299..2754)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2436..2457)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2648..2667
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2781..2885
/gene="bla"
/label=AmpR promoter
promoter 2887..3244
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2887..3078
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2910..2930)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3095..3230
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3279..4073
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3279..4073
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3333..3352)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3943..3962
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4261..4280)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4305..4352
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4681..5269
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5170..5189
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
61 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
121 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
181 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
421 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
481 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
541 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
601 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
661 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
721 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
781 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
841 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
901 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
961 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
1021 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
1081 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
1141 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
1201 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
1261 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
1321 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
1381 ggacgagctg tacaagtccg gactcagatc tcgagccatg acctctagga agaaagtgtt
1441 gctgaaggtt atcatcctgg gagattctgg agtcgggaag acatcactca tgaaccagta
1501 tgtgaataag aaattcagca atcagtacaa agccacaata ggagctgact ttctgaccaa
1561 ggaggtgatg gtggatgaca ggctagtcac aatgcagata tgggacacag caggacagga
1621 acggttccag tctctcggtg tggccttcta cagaggtgca gactgctgcg ttctggtatt
1681 tgatgtgact gcccccaaca cattcaaaac cctagatagc tggagagatg agtttctcat
1741 ccaggccagt ccccgagatc ctgaaagctt cccatttgtt gtgttgggaa acaagattga
1801 cctcgaaaac agacaagtgg ccacaaagcg ggcacaggcc tggtgctaca gcaaaaacaa
1861 cattccctac tttgagacca gtgccaagga ggccatcaac gtggagcagg cgttccagac
1921 gattgcacgg aatgcactta agcaggaaac ggaggtggag ctgtacaacg aatttcctga
1981 acctatcaaa ctggacaaga atgaccgggc caaggcctcg gcagaaagct gcagttgctg
2041 aggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
2101 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
2161 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
2221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
2281 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
2341 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
2401 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
2461 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
2521 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
2581 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
2641 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
2701 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
2761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
2821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
2881 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
2941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
3001 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
3061 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
3121 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
3181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
3241 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
3301 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
3361 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
3421 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
3481 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
3541 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
3601 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
3661 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
3721 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
3781 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
3841 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
3901 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
3961 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
4021 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
4081 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
4141 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
4201 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
4261 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
4321 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
4381 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
4441 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
4501 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
4561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
4621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
4681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
4741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
4801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
4861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
4921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
4981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
5041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
5101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
5161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
5221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
5281 cgcggccttt ttacggttcc tggcctt
//
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