pcDNA3-Lyn-ICUE3

LOCUS       pcDNA3-Lyn-ICUE3        9123 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Plasma membrane-targeted FRET biosensor for monitoring cAMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   DiPilato LM, Zhang J
  TITLE     The role of membrane microdomains in shaping beta2-adrenergic 
            receptor-mediated cAMP dynamics.
  JOURNAL   Mol Biosyst. 2009 Aug;5(8):832-7. doi: 10.1039/b823243a. Epub 2009 
            May 26.
  PUBMED    19603118
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b823243a"; 
            journalName: "Mol Biosyst"; date: "2009-08"; volume: "5"; issue: 
            "8"; pages: "832-7"
COMMENT     Sequence Label: pcDNA3-Lyn-ICUE3
FEATURES             Location/Qualifiers
     source          1..9123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(946..948,949..951,952..1662)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(946..948,949..951,952..1662)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(946..948,949..951,952..1662)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(4027..4029,4030..4032,4033..4545)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     promoter        complement(4675..4693)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4676..4693)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4713..4730)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4719..4943
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4989..5417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5076..5095)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5286..5307
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5426..5446)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5431..5761
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5612..5747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5674..5693
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5828..6622
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5882..5901)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6492..6511
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6796..6917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6833..6852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6887..6906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6966..6982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6966..6982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6979..7001)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6990..7006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7014..7020,7021..7038,7039..7044))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7059..7080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7197..7214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7368..7956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7448..7467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8127..8918,8919..8987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8750..8769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8988..9092)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg gctgcatcaa gagcaagcgc aaggacaagg atcccatggt gagcaagggc
      961 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1021 cacaggttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1081 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1141 acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1201 aagtccgcca tgcccgaagg ctacgtccag gagcgtacca tcttcttcaa ggacgacggc
     1261 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1321 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1381 tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggcccac
     1441 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1501 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1561 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1621 accgccgccg ggatcactca cggcatggac gagctgtaca aggctggtac ccccgtggga
     1681 actcatgaga tggaggagga gttggccgaa gctgtggccc tgctctccca gcgggggcct
     1741 gacgccctgc tcactgtggc acttcgaaag cccccaggtc agcgcacgga tgaagagctg
     1801 gacctcatct ttgaggagct gctgcacatc aaggctgtgg cccacctctc caactcggtg
     1861 aagcgagaat tagcggctgt tctgctcttt gaaccacaca gcaaggcagg gaccgtgttg
     1921 ttcagccagg gggacaaggg cacttcgtgg tacattatct ggaagggatc tgtcaacgtg
     1981 gtgacccatg gcaaggggct ggtgaccacc ctgcatgagg gagatgattt tggacagctg
     2041 gctctggtga atgatgcacc ccgggcagcc accatcatcc tgcgagaaga caactgtcat
     2101 ttcctgcgtg tggacaagca ggacttcaac cgtatcatca aggatgtgga ggcaaagacc
     2161 atgcggctgg aagaacatgg caaagtggtg ctggtgctgg agagagcctc tcagggcgcc
     2221 ggcccttccc gacccccaac cccaggcagg aaccggtata cagtgatgtc tggcacccca
     2281 gagaagatcc tagagcttct gttggaggcc atgggaccag attccagtgc tcatgaccaa
     2341 acagagacat tcctcagcga cttcctcctg acccacaggg tcttcatgcc cagcgcccaa
     2401 ctctgcgctg cccttctgca ccacttccat gtggagcctg cgggtggcag cgagcaggag
     2461 cgcagcacct acgtctgcaa caagaggcag cagatcttgc ggctggtcag ccagtgggtg
     2521 gccctgtatg gctccatgct ccacactgac cctgtggcca ccagcttcct ccagaaactc
     2581 tcagacctgg tgggcaggga cacccgactc agcaacctgc tgagggagca gtggccagag
     2641 aggcggcgat gccacaggtt ggagaatggc tgtgggaatg catctcctca gatgaaggcc
     2701 cggaacttgc ctgtttggct ccccaaccag gacgagcccc ttcctggcag cagctgtgcc
     2761 atccaagttg gggataaagt cccctatgac atctgccggc cagaccactc agtgttgacc
     2821 ctgcagctgc ctgtgacagc ctccgtgaga gaggtgatgg cagcgttggc ccaggaggat
     2881 ggctggacca aggggcaggt gctggtgaag gtcaattctg caggtgatgc cattggcctg
     2941 cagccagatg cccgtggtgt ggccacatct ctggggctca atgagcgtct ctttgttgtc
     3001 aacccacagg aagcgcatga gctgatccca caccctgacc agctggggcc cactgtgggc
     3061 tctgctgagg ggctggacct ggtgagtgcc aaggacctgg caggccagct gacggaccac
     3121 gactggagcc tcttcaacag tatccaccag gtggagctga tccactatgt gctgggcccc
     3181 cagcatctgc gggatgtcac caccgccaac ctggagcgct tcatgcgccg cttcaatgag
     3241 ctgcagtact gggtggccac cgagctgtgt ctctgccccg tgcccggccc ccgggcccag
     3301 ctgctcagga agttcattaa gctggcggcc cacctcaagg agcagaagaa tctcaattcc
     3361 ttctttgccg tcatgtttgg cctcagcaac tcggccatca gccgcctagc ccacacctgg
     3421 gagcggctgc cccacaaagt ccggaagctg tactccgccc tcgagaggct gctggacccc
     3481 tcatggaacc accgggtata ccgactggcc ctcgccaagc tctcccctcc tgtcatcccc
     3541 ttcatgcccc ttcttctcaa agacatgacc ttcattcatg agggaaacca cacactagtg
     3601 gagaatctca tcaactttga gaagatgaga atgatggcca gagccgcgcg gatgctgcac
     3661 cactgccgaa gccacaaccc tgtgcctctc tcaccactca gaagccgagt ttcccacctc
     3721 cacgaggaca gccaggtggc gaggatttcc acatgctcgg agcagtccct gagcacccgg
     3781 agtccagcca gcacctgggc ttatgtccag cagctgaagg tcattgacaa ccagcgggaa
     3841 ctctcccgcc tctcccgaga gctggagcca gagctcatgc ccgacaacca ctncctgagc
     3901 taccagncca agntgagcaa agaccccaac gagaagcgcg atcacatggt cntgctggag
     3961 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aggtaccggt
     4021 ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4081 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4141 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     4201 cccaccctcg tgaccaccct gggctacggc cttcagtgct tcgcccgcta ccccgaccac
     4261 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4321 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4381 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4441 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     4501 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     4561 ctcgccgacc actaccagca gaacaccccc nnnggngacg gccccgtgct gtaagaattc
     4621 tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc ctattctata
     4681 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     4741 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4801 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4861 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4921 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4981 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5041 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5101 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
     5161 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5221 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5281 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5341 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
     5401 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5461 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5521 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5581 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5641 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5701 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5761 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     5821 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5881 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5941 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     6001 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     6061 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     6121 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6181 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6241 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6301 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6361 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6421 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6481 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6541 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6601 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     6661 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6721 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6781 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6901 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7021 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7081 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7141 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7561 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7801 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8101 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9001 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9061 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9121 gtc
//