| 质粒名称: | pC1-HyPerFLEX-ERlum |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 238941 |
| 质粒用途: | Mammalian expression of HyPerFLEX targeted to the luminal side of the endoplasmic reticulum membranes |
| 存储实验室: | Vsevolod Belousov |
|
载体骨架基本信息 |
|
| 载体名称: | pC1 |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HyPerFLEX-ERlum (Calreticulin signal sequence-HyPerFLEX-KDEL) |
| 物种来源: | Synthetic |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | 未知（如果被销毁，则未知） |
|
文献引用方法 |
|
| 材料和方法部分: | pC1-HyPerFLEX-ERlum was a gift from Vsevolod Belousov (Addgene plasmid # 238941 ; http://n2t.net/addgene:238941 ; RRID:Addgene_238941) |
| 参考文献部分: | A color-tailored fluorogenic sensor for hydrogen peroxide. Potekhina ES, Bass DI, Ezerina D, Fleckenstein DD, Chebotarev AS, Sysoeva VA, Maltsev DI, Pak VV, Moshchenko AA, Sokolov AI, Myasnyanko IN, Baranov MS, Fedotov AB, Zheltikov AM, Lanin AA, Messens J, Belousov VV. Nat Chem Biol. 2025 Oct 16. doi: 10.1038/s41589-025-02036-6. 10.1038/s41589-025-02036-6 PubMed 41102408 |
pC1-HyPerFLEX-ERlum
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
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买家导航
pC1-HyPerFLEX-ERlum载体质粒基本信息
pC1-HyPerFLEX-ERlum质粒图谱载体图谱和pC1-HyPerFLEX-ERlum载体序列质粒序列多克隆位点信息
pC1-HyPerFLEX-ERlum质粒载体简介
pC1-HyPerFLEX-ERlum质粒序列载体序列
LOCUS pC1-HyPerFLEX-ERlum 5152 bp DNA circular SYN 04-SEP-2026
DEFINITION Mammalian expression of HyPerFLEX targeted to the luminal side of
the endoplasmic reticulum membranes .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5152)
AUTHORS Potekhina ES, Bass DI, Ezerina D, Fleckenstein DD, Chebotarev AS,
Sysoeva VA, Maltsev DI, Pak VV, Moshchenko AA, Sokolov AI,
Myasnyanko IN, Baranov MS, Fedotov AB, Zheltikov AM, Lanin AA,
Messens J, Belousov VV
TITLE A color-tailored fluorogenic sensor for hydrogen peroxide.
JOURNAL Nat Chem Biol. 2025 Oct 16. doi: 10.1038/s41589-025-02036-6.
PUBMED 41102408
REFERENCE 2 (bases 1 to 5152)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 4, 2026 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem
Biol. 2025 Oct 16. doi: 10.1038/s41589-025-02036-6."
COMMENT Sequence Label: pC1-HyPerFLEX-ERlum
FEATURES Location/Qualifiers
source 1..5152
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
polyA_signal 1940..2061
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1977..1996)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2031..2050
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2068..2523)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2205..2226)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2417..2436
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2550..2654
/gene="bla"
/label=AmpR promoter
promoter 2656..3013
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2656..2847
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2679..2699)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2864..2999
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3048..3842
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3048..3842
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3102..3121)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3712..3731
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4030..4049)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4074..4121
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4450..5038
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4939..4958
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgc
601 tgctatccgt gccgttgctg ctcggcctcc tcggcctggc cgtcgccgga ccggtcgcca
661 cccacctggc taatgaggag caaaacgaat tggaaggtgc ttttaagctg ggcttgatct
721 ttaccgtcgc cccgtacttg ctgcctaagt tgatcgtcag cctgcgccgc accgccccga
781 agatgccttt gatgttggag gagaattaca cccacaccct taccgagtca ctgaaacgcg
841 gcgacgttga cgccatcatt gtggcagagc cgtttcaaga gccgggtatt gtgactgaac
901 ctttgtatga cgagccgttt ttcgttatcg ttccgaaggg ccatagcttc gaggagttgg
961 atgccgttag ccctcgcatg ttgggcgaag aacaagttct gcttctgacc gagggcaact
1021 gtatgcgtga ccaggtgctt agcattttta ccaaggtcaa ggtgcacatg aagaaagccc
1081 tttccggtga cagctattgg gtctttgtga aacgggtagg tggcactggc ggtgagcatg
1141 ttgtctttgg cagtgaggac atcgagaaca ctatggccaa aatggacgac ggacaactgg
1201 atgggttggc ctttggcgca attcagctcg atggtgacgg gaatatcctg cagtacaatg
1261 ctgctgaagg agacatcaca ggcagagatc ccaaacaggt gattgggaag aacttcttca
1321 aggatgttgc acctggaacg gattctcccg agttttacgg caaattcaag gaaggcgtag
1381 cgtcagggaa tctgaacacc atgttcgaat ggatgatacc gactcttacc gatcatagtt
1441 gcagcgagct tgccgccaaa cagcgcattc agggtctcac caacaccctt caaggctcaa
1501 gcatcgatac catccgccac atggttgcca gtggcttggc aatcagcgtc ttgcctgcca
1561 ctgcccttac cgaaaacgac cacatgctgt ttagcattat cccgtttgaa ggcaccccgc
1621 ctagccgccg cgttgtcctg gcctatcgcc gcaactttgt ccgccctaag gccctttcag
1681 ccatgaaagc cgcaattatg caaagccaac tgcatggtgt tagcttcatc tgcgatggtg
1741 gaggaggtag atctggaaag gacgagctgt aaaagcttcg aattctgcag tcgacggtac
1801 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
1861 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
1921 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
1981 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
2041 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
2161 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
2281 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
2521 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
2581 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
2641 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
2701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2761 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2821 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
2881 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
2941 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
3001 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
3061 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
3121 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
3181 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
3241 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
3301 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
3361 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
3421 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
3481 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
3541 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
3601 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
3661 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
3721 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
3781 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
3841 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
3901 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
3961 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
4021 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
4081 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
4141 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
4201 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
4261 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
4321 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
4381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
4441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
4501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
4561 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
4621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
4681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
4741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
4801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
4861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
4921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
4981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
5041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
5101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
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