pCDH-CMV-mCherry-CSDE1

LOCUS       pCDH-CMV-mCherry-CSDE1            9453 bp DNA     circular SYN 14-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2909..2928
                     /label=mCherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3219..3257
                     /label=Primer F for CSDE1 in mCherry
     CDS             3240..5540
                     /codon_start=1
                     /label=CSDE1 soform2 104-134 missing
                     /translation="MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSER
                     QARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERMNGQE
                     VFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARCQGVVCAMK
                     EAFGFIERGDVVKEIFFHYSEFKGDLETLQPGDDVEFTIKDRNGKEVATDVRLLPQGTV
                     IFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLE
                     GDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDVR
                     MFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLG
                     TVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKV
                     EFSISDKQRPGQQVATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSE
                     FSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRS
                     VDPTQTEYQGMIEIVEEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTM
                     AYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQ
                     RTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMG
                     FGAERKIRQAGVID"
     primer_bind     complement(4889..4906)
                     /label=yzy-P765
     misc_feature    complement(5523..5561)
                     /label=Priemr R for CSDE1 in mCherry
     misc_feature    5550..6137
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6021..6032)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6210..6443
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6515..6636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6655..6790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6828..6844)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6852..6868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6876..6906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6921..6942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7230..7818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7989..8849)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8850..8954)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9428..9444
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tgagctttga tccaaacctt ctccacaaca atggacataa tgggtaccct aatggtactt
     3301 cagcagcact gcgtgaaact ggggttattg aaaaactgtt aacctcttac ggatttattc
     3361 agtgttcaga acgtcaagct agacttttct tccactgttc acagtataat ggcaacctgc
     3421 aagacttaaa agtaggagat gatgttgaat ttgaagtatc atcggaccga cggactggga
     3481 aacccattgc tgttaaactg gtgaagataa aacaagaaat cctccctgaa gaacgaatga
     3541 atggacaaga agtgttttat ctgacttaca cccctgaaga tgtcgaaggg aacgttcagc
     3601 tggaaactgg agataaaata aactttgtaa ttgataacaa taaacatact ggtgctgtaa
     3661 gtgctcgcaa cattatgctg ttgaaaaaga aacaagcccg ctgtcaggga gtagtttgtg
     3721 ccatgaagga ggcatttggc tttattgaaa gaggtgatgt tgtaaaagag atattctttc
     3781 actatagtga atttaagggt gacttagaaa ccttacagcc tggcgatgat gtggaattca
     3841 caatcaagga cagaaatggt aaagaagttg caacagatgt cagactattg cctcaaggaa
     3901 cagtcatttt tgaagatatc agcattgaac attttgaagg aactgtaacc aaagttatcc
     3961 caaaagtacc cagtaaaaac cagaatgacc cattgccagg acgcatcaaa gttgactttg
     4021 tgatccctaa agaacttccc tttggagaca aagatacgaa atccaaggtg accctgctgg
     4081 aaggtgacca tgttaggttt aatatttcaa cagaccgacg tgacaaatta gagcgagcaa
     4141 ccaatataga agttctgtca aatacatttc agttcactaa tgaagcccga gaaatgggtg
     4201 tgattgctgc catgagagat ggttttggtt tcatcaagtg tgtggatcgt gatgttcgta
     4261 tgttcttcca cttcagtgaa attctggatg ggaaccagct ccatattgca gatgaagtag
     4321 agtttactgt ggttcctgat atgctctctg ctcaaagaaa tcatgctatt aggattaaaa
     4381 aacttcccaa gggcacggtt tcatttcatt cccattcaga tcaccgtttt ctgggcacgg
     4441 tagaaaaaga agccactttt tccaatccta aaaccactag cccaaataaa ggcaaagaga
     4501 aggaggctga ggatggcatt attgcttatg atgactgtgg ggtgaaactg actattgctt
     4561 ttcaagccaa ggatgtggaa ggatctactt ctcctcaaat aggagataag gttgaattta
     4621 gtattagtga caaacagagg cctggacagc aggttgcaac ttgtgtgcga cttttaggtc
     4681 gtaattctaa ctccaagagg ctcttgggtt atgtggcaac tctgaaggat aattttggat
     4741 ttattgaaac agccaatcat gataaggaaa tctttttcca ttacagtgag ttctctggtg
     4801 atgttgatag cctggaactg ggggacatgg tcgagtatag cttgtccaaa ggcaaaggca
     4861 acaaagtcag tgcagaaaaa gtgaacaaaa cacactcagt gaatggcatt actgaggaag
     4921 ctgatcccac catttactct ggcaaagtaa ttcgccccct gaggagtgtt gatccaacac
     4981 agactgagta ccaaggaatg attgagattg tggaggaggg cgatatgaaa ggtgaggtct
     5041 atccatttgg catcgttggg atggccaaca aaggggattg cctgcagaaa ggggagagcg
     5101 tcaagttcca attgtgtgtc ctgggccaaa atgcacaaac tatggcttac aacatcacac
     5161 ccctgcgcag ggccacagtg gaatgtgtga aagatcagtt tggcttcatt aactatgaag
     5221 taggagatag caagaagctc tttttccatg tgaaagaagt tcaggatggc attgagctac
     5281 aggcaggaga tgaggtggag ttctcagtga ttcttaatca gcgcactggc aagtgcagcg
     5341 cctgtaatgt ttggcgagtc tgtgagggcc ccaaggctgt tgcagctcct cgacctgatc
     5401 ggttggtcaa tcgcttgaag aatatcactc tggatgatgc cagtgctcct cgcctaatgg
     5461 ttcttcgtca gccaagggga ccagataact caatggggtt tggtgcagaa agaaagatcc
     5521 gtcaagctgg tgtcattgac tagggatcca atcaacctct ggattacaaa atttgtgaaa
     5581 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5641 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5701 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5761 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5821 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5881 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5941 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6001 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6061 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
     6121 tttggccgcc tccccgccgg tacctttaag accaatgact tacaaggcag ctgtagatct
     6181 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata
     6241 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6301 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6361 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6421 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6481 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     6541 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6601 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     6661 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6721 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6781 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt
     6841 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6901 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6961 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7021 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7081 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7141 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7201 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7261 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7321 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7381 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7441 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7501 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7561 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7621 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7681 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7741 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7801 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7861 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7921 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7981 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8041 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8101 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8161 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8221 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8281 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8341 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8401 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8461 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8521 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8581 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8641 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8701 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8761 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8821 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8881 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8941 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     9001 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     9061 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     9121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     9181 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     9241 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     9301 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     9361 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     9421 cgacgttgta aaacgacggc cagtgccaag ctg
//