| 质粒名称: | pYK506 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000824 |
| 产品用途: | Mammalian PhCMV-driven constitutive 4xANR-CAAX expression vector (PhCMV-4xANR-CAAX-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | 4xANR-CAAX |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK506
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK506载体质粒基本信息
pYK506质粒图谱载体图谱和pYK506载体序列质粒序列多克隆位点信息
pYK506质粒载体简介
pYK506质粒序列载体序列
LOCUS pYK506 6109 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6109)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6109
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..930
/label=oYK474_ANR_F
primer_bind complement(881..900)
/label=oYK236_Nhe_R
primer_bind 881..899
/label=oYK06-ANR-mEGFP-F
primer_bind 904..953
/label=olyl215
misc_feature 904
/label=NheI
primer_bind 915..953
/label=olyl211
primer_bind complement(915..934)
/label=olyl323
misc_feature 915
/label=New Feature
misc_feature 915
/label=New Feature
CDS 928
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 929..991
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 929..961
/label=olyl235
CDS 992..1066
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1015..1024
/label=oYK474_mCherry_F
CDS 1067..1129
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1067..1082
/label=Primer 4
primer_bind 1114..1128
/label=Primer 3
CDS 1130..1204
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1130..1148
/label=Primer 1
primer_bind complement(1179..1208)
/label=olyl234
primer_bind complement(1179..1204)
/label=olyl212
primer_bind 1189..1243
/label=olyl235
CDS 1211..1273
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1211..1235
/label=olyl211
primer_bind 1211..1235
/label=olyl215
CDS 1274..1348
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1297..1306
/label=oYK474_mCherry_F
CDS 1349..1411
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1349..1364
/label=Primer 4
primer_bind 1396..1410
/label=Primer 3
CDS 1412..1486
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1412..1430
/label=Primer 1
primer_bind complement(1461..1505)
/label=olyl212
primer_bind complement(1461..1505)
/label=olyl234
primer_bind 1471..1490
/label=olyl235
primer_bind complement(1480..1524)
/label=oYK506_ANR_R
primer_bind complement(1480..1504)
/label=oYK474_ANR4_R
primer_bind 1485..1504
/label=oYK474_mCherry_F
primer_bind complement(1487..1505)
/label=olyl236
misc_feature 1493..1504
/label=NSP3
primer_bind complement(1496..1513)
/label=oYK492_ANR4_R
CDS 1504
/codon_start=1
/label=GNCR1-N
/translation=""
primer_bind 1505..1521
/label=oYK511_CAAX_F
primer_bind complement(1505..1520)
/label=oYK277_ANR_R
primer_bind complement(1510..1596)
/label=oYK506_CAAX_R1
misc_feature 1544..1603
/label=CAAX
primer_bind 1547..1582
/label=oYK491_CAAX_F
primer_bind complement(1563..1629)
/label=oYK421_CAAX_R
terminator 1703..1930
/label=bGH PA
misc_feature 1976..2404
/label=f1 origin
misc_feature 2409..2752
/label=SV40 early promoter and origin
misc_feature 2598..2675
/label=SV40 ORI
CDS 2817..3608
/label=Kan/neoR
misc_feature 3782..3912
/label=SV40 early polyadenylation signal
primer_bind complement(3948..3968)
/label=M13-rev
misc_binding complement(3974..3996)
/label=LacO
promoter complement(4001..4030)
/label=lac
misc_feature complement(4295..4968)
/label=pUC origin
CDS complement(5116..5775)
/label=AmpR
promoter complement(6015..6043)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccgtttaa acttagcttg ccaccatggg ggagctcgac gagttggttt atctcttaga
961 cggacccgga tatgacccca ttcattcaga tggctccgga acgggcagcg ggaccggaag
1021 tggcaccgga accacttccg gaaccggtac tggcgggagt accggcggtg agctagacga
1081 actcgtgtac ctgttggatg gccccggtta cgacccaatc cacagcgatg gaagtggaac
1141 tgggtctggg actgggtccg gcaccgggac tacttcaggc accggcactg ggggctccac
1201 tggccatacc ggggagctcg acgagttggt ttatctctta gacggacccg gatatgaccc
1261 cattcattca gatggctccg gaacgggcag cgggaccgga agtggcaccg gaaccacttc
1321 cggaaccggt actggcggga gtaccggcgg tgagctagac gaactcgtgt acctgttgga
1381 tggccccggt tacgacccaa tccacagcga tggaagtgga actgggtctg ggactgggtc
1441 cggcaccggg actacttcag gcaccggcac tgggggctcc actggccata tgatgctcaa
1501 gatggccagc ggttccggac gggctgacgt cggcagcggc agcaaaatga gtaaggatgg
1561 aaagaaaaag aaaaagaaaa gcaaaacaaa gtgtgtaata atgtaaggat ccactagtcc
1621 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
1681 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
1741 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
1801 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
1861 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
1921 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
1981 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
2041 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
2101 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
2161 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
2221 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
2281 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
2341 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
2401 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
2461 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
2521 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
2581 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
2641 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
2701 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
2761 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
2821 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
2881 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
2941 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
3001 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
3061 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
3121 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
3181 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
3241 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
3301 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
3361 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
3421 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
3481 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
3541 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
3601 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
3661 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
3721 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
3781 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
3841 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
3901 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
3961 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
4021 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
4081 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
4141 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
4201 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
4261 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
4321 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
4381 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4441 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
4501 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4561 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4621 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
4681 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
4741 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
4801 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4861 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
4921 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
4981 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
5041 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
5101 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
5161 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
5221 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
5281 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
5341 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
5401 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
5461 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
5521 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
5581 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
5641 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
5701 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
5761 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
5821 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
5881 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
5941 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
6001 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
6061 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
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