psiCHECK-2-h1-cluster04

LOCUS       psiCHECK-2-h1-cluster04            6608 bp DNA     circular SYN 06-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2007
                     /label=h1-cluster04
     polyA_signal    2023..2071
                     /note="synthetic polyadenylation signal"
     promoter        2080..2831
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2861..2870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2867..4519
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3826..3834
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4563..4684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4817..4921
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4922..5782
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5953..6541
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 atatatatta tatataatat atataatata ttatatgtat tatatataat atatattata
     1741 tattatatgt attatatata atatatatta tatattatat atagtgtatt ttgatttttt
     1801 aaatgcacct ggctcctcta gtaaatgcca gtttttcaga attttgtgat gacattttta
     1861 tcatctctga ctcttcagta cctgaaagat attaaggagt gaatatttta tagaatattt
     1921 attgaatgag tgtattagtc tgttctcacg ctgctaataa agacataccc gagactgggc
     1981 aacttataaa ggaaagaggt ttaatgggcg gccgctggcc gcaataaaat atctttattt
     2041 tcattacatc tgtgtgttgg ttttttgtgt gaggatctaa atgagtcttc ggacctcgcg
     2101 ggggccgctt aagcggtggt tagggtttgt ctgacgcggg gggaggggga aggaacgaaa
     2161 cactctcatt cggaggcggc tcggggtttg gtcttggtgg ccacgggcac gcagaagagc
     2221 gccgcgatcc tcttaagcac ccccccgccc tccgtggagg cgggggtttg gtcggcgggt
     2281 ggtaactggc gggccgctga ctcgggcggg tcgcgcgccc cagagtgtga ccttttcggt
     2341 ctgctcgcag acccccgggc ggcgccgccg cggcggcgac gggctcgctg ggtcctaggc
     2401 tccatgggga ccgtatacgt ggacaggctc tggagcatcc gcacgactgc ggtgatatta
     2461 ccggagacct tctgcgggac gagccgggtc acgcggctga cgcggagcgt ccgttgggcg
     2521 acaaacacca ggacggggca caggtacact atcttgtcac ccggaggcgc gagggactgc
     2581 aggagcttca gggagtggcg cagctgcttc atccccgtgg cccgttgctc gcgtttgctg
     2641 gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac acaaaccccg
     2701 cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg gcgcggtccc
     2761 aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc
     2821 gacccgctta aaagcttggc attccggtac tgttggtaaa gccaccatgg ccgatgctaa
     2881 gaacattaag aagggccctg ctcccttcta ccctctggag gatggcaccg ctggcgagca
     2941 gctgcacaag gccatgaaga ggtatgccct ggtgcctggc accattgcct tcaccgatgc
     3001 ccacattgag gtggacatca cctatgccga gtacttcgag atgtctgtgc gcctggccga
     3061 ggccatgaag aggtacggcc tgaacaccaa ccaccgcatc gtggtgtgct ctgagaactc
     3121 tctgcagttc ttcatgccag tgctgggcgc cctgttcatc ggagtggccg tggcccctgc
     3181 taacgacatt tacaacgagc gcgagctgct gaacagcatg ggcatttctc agcctaccgt
     3241 ggtgttcgtg tctaagaagg gcctgcagaa gatcctgaac gtgcagaaga agctgcctat
     3301 catccagaag atcatcatca tggactctaa gaccgactac cagggcttcc agagcatgta
     3361 cacattcgtg acatctcatc tgcctcctgg cttcaacgag tacgacttcg tgccagagtc
     3421 tttcgacagg gacaaaacca ttgccctgat catgaacagc tctgggtcta ccggcctgcc
     3481 taagggcgtg gccctgcctc atcgcaccgc ctgtgtgcgc ttctctcacg cccgcgaccc
     3541 tattttcggc aaccagatca tccccgacac cgctattctg agcgtggtgc cattccacca
     3601 cggcttcggc atgttcacca ccctgggcta cctgatttgc ggctttcggg tggtgctgat
     3661 gtaccgcttc gaggaggagc tgttcctgcg cagcctgcaa gactacaaaa ttcagtctgc
     3721 cctgctggtg ccaaccctgt tcagcttctt cgctaagagc accctgatcg acaagtacga
     3781 cctgtctaac ctgcacgaga ttgcctctgg cggcgcccca ctgtctaagg aggtgggcga
     3841 agccgtggcc aagcgctttc atctgccagg catccgccag ggctacggcc tgaccgagac
     3901 aaccagcgcc attctgatta ccccagaggg cgacgacaag cctggcgccg tgggcaaggt
     3961 ggtgccattc ttcgaggcca aggtggtgga cctggacacc ggcaagaccc tgggagtgaa
     4021 ccagcgcggc gagctgtgtg tgcgcggccc tatgattatg tccggctacg tgaataaccc
     4081 tgaggccaca aacgccctga tcgacaagga cggctggctg cactctggcg acattgccta
     4141 ctgggacgag gacgagcact tcttcatcgt ggaccgcctg aagtctctga tcaagtacaa
     4201 gggctaccag gtggccccag ccgagctgga gtctatcctg ctgcagcacc ctaacatttt
     4261 cgacgccgga gtggccggcc tgcccgacga cgatgccggc gagctgcctg ccgccgtcgt
     4321 cgtgctggaa cacggcaaga ccatgaccga gaaggagatc gtggactatg tggccagcca
     4381 ggtgacaacc gccaagaagc tgcgcggcgg agtggtgttc gtggacgagg tgcccaaggg
     4441 cctgaccggc aagctggacg cccgcaagat ccgcgagatc ctgatcaagg ctaagaaagg
     4501 cggcaagatc gccgtgtaat aattctagag tcggggcggc cggccgcttc gagcagacat
     4561 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     4621 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4681 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     4741 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc caggtggcac
     4801 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4861 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4921 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4981 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5041 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5101 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5161 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5221 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5281 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5341 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5401 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5461 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5521 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5581 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5641 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5701 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5761 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5821 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5881 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5941 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6001 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6061 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6121 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6181 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6241 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6301 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6361 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6421 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6481 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6541 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6601 ggctcgac
//