| 出品公司: | Invivogen |
|---|---|
| 载体名称: | pFUSE-hIgG1e2-Fc1 |
| 载体又名: | pFUSE-hIgG1e2-Fc1载体，pFUSE-hIgG1e2-Fc1质粒，pFUSE-hIgG1e2-Fc1载体质粒 |
| 英文名称: | pFUSE-hIgG1e2-Fc1, pFUSE-hIgG1e2-Fc1 Vector, pFUSE-hIgG1e2-Fc1 Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4.1 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | Zeocin |
| 筛选标记: | |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: |
pFUSE-hIgG1e2-Fc1载体说明书 pFUSE-hIgG1e2-Fc1载体序列 |
| 产品目录号: | 217548512 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pFUSE-hIgG1e2-Fc1载体质粒基本信息
pFUSE-hIgG1e2-Fc1质粒图谱载体图谱和pFUSE-hIgG1e2-Fc1载体序列质粒序列多克隆位点信息
pFUSE-hIgG1e2-Fc1质粒载体简介
pFUSE-hIgG1e2-Fc1质粒序列载体序列
pFUSE-hIgG1e2-Fc1 Snapgene格式序列 pFUSE-hIgG1e2-Fc1 .gb格式序列
LOCUS pFUSE-hIgG1e2-Fc1 4132 bp DNA circular SYN 31-JUL-2025
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4132)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jul 31, 2025 from SnapGene 8.1.0
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4132
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 31..242
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
/note="color: #ffffff; direction: RIGHT"
LTR 255..523
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
/note="color: #ffe4c4"
polyA_signal 1297..1418
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
polyA_signal 1522..1916
/label=beta-globin poly(A)
/note="human beta-globin polyadenylation signal"
/note="color: #a6acb3"
CDS complement(1930..2304)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/note="color: #ccffcc"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
enhancer 2897..3200
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
/note="color: #ffffff"
rep_origin complement(3280..3868)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
polyA_signal 3976..4024
/note="synthetic polyadenylation signal"
/note="color: #a6acb3"
misc_feature 4035..4126
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
/note="color: #a6acb3"
ORIGIN
1 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
61 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
541 ctacctgaga tcaccggtga attcgatatc tcgagcacca tggttagatc tgacaaaact
601 cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt cttcctcttc
661 cccccaaaac ccaaggacac cctctacatc acccgggaac ctgaggtcac atgcgtggtg
721 gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag
781 gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc
841 agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc
901 tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc
961 cgagaaccac aggtgtacac cctgccccca tcccgggagg agatgaccaa gaaccaggtc
1021 agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc
1081 aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc
1141 ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc
1201 tcatgctccg tgatgcatga ggctctgaag ttccactaca cgcagaagag cctctccctg
1261 tctccgggta aatgagtgct agctggccag acatgataag atacattgat gagtttggac
1321 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
1381 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
1441 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
1501 aatgtggtat ggaattaatt ctaaaataca gcatagcaaa actttaacct ccaaatcaag
1561 cctctacttg aatccttttc tgagggatga ataaggcata ggcatcaggg gctgttgcca
1621 atgtgcatta gctgtttgca gcctcacctt ctttcatgga gtttaagata tagtgtattt
1681 tcccaaggtt tgaactagct cttcatttct ttatgtttta aatgcactga cctcccacat
1741 tcccttttta gtaaaatatt cagaaataat ttaaatacat cattgcaatg aaaataaatg
1801 ttttttatta ggcagaatcc agatgctcaa ggcccttcat aatatccccc agtttagtag
1861 ttggacttag ggaacaaagg aacctttaat agaaattgga cagcaagaaa gcgagcttct
1921 agcttatcct cagtcctgct cctctgccac aaagtgcacg cagttgccgg ccgggtcgcg
1981 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
2041 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
2101 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
2161 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
2221 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
2281 aacggcactg gtcaacttgg ccatgatggc tcctcctgtc aggagaggaa agagaagaag
2341 gttagtacaa ttgctatagt gagttgtatt atactatgca gatatactat gccaatgatt
2401 aattgtcaaa ctagggctgc agggttcata gtgccacttt tcctgcactg ccccatctcc
2461 tgcccaccct ttcccaggca tagacagtca gtgacttacc aaactcacag gagggagaag
2521 gcagaagctt gagacagacc cgcgggaccg ccgaactgcg aggggacgtg gctagggcgg
2581 cttcttttat ggtgcgccgg ccctcggagg cagggcgctc ggggaggcct agcggccaat
2641 ctgcggtggc aggaggcggg gccgaaggcc gtgcctgacc aatccggagc acataggagt
2701 ctcagccccc cgccccaaag caaggggaag tcacgcgcct gtagcgccag cgtgttgtga
2761 aatgggggct tgggggggtt ggggccctga ctagtcaaaa caaactccca ttgacgtcaa
2821 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
2881 gccaaaaccg catcatcatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
2941 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
3001 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
3061 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
3121 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
3181 ttaccgtaag ttatgtaacg cctgcaggtt aattaagaac atgtgagcaa aaggccagca
3241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
3301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
3361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
3421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
3481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
3541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
3601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
3661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
3721 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
3781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
3841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
3901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atggctagtt aattaacatt
3961 taaatcagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
4021 tgtgaatcgt aactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
4081 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg aa
//
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