| 质粒名称: | pJWK-FtsZ-mBiyu |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002670 |
| 产品用途: | Expressing FtsZ-mBiyu in D. radiodurans for Z-ring visualization |
| 存储实验室: | Xinxing Yang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pJWK |
| 表达系统: | 大肠杆菌表达 |
| 启动子: |
LtetO-1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DrFtsZ-mBiyu |
| 物种来源: | synthetic |
| 密码子优化: | Dradiodurans |
pJWK-FtsZ-mBiyu
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pJWK-FtsZ-mBiyu载体质粒基本信息
pJWK-FtsZ-mBiyu质粒图谱载体图谱和pJWK-FtsZ-mBiyu载体序列质粒序列多克隆位点信息
pJWK-FtsZ-mBiyu质粒载体简介
pJWK-FtsZ-mBiyu质粒序列载体序列
LOCUS pJWK-FtsZ-mBiyu 6555 bp DNA circular SYN 28-MAY-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6555)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6555
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..683
/label=ColE1 origin
misc_feature 720..725
/label=HindIII
promoter 756..829
/locus_tag="pLtetO-1"
/label=pLtetO-1 promoter
protein_bind 756..774
/locus_tag="TetO2"
-35_signal 775..780
/locus_tag="-33"
protein_bind 781..799
/locus_tag="TetO2(1)"
-10_signal 798..803
/locus_tag="-10"
misc_feature 810
/locus_tag="pLtetO-1 +1"
RBS 839..850
/label=RBS
/note="from pSD5-lacZ"
CDS 859..861
/codon_start=1
/label=DrPBP2-1
/translation="M"
CDS 862..1971
/codon_start=1
/label=DrFtsZ
/translation="QAARIRVIGLGGAGNNAVNRMIESGLEGVEFIAGNTDAQVLAKSH
AEVRIQLGDRLTRGLGAGADPKVGEEAAVEDRDRIKEYLDDTDMLFITAGMGGGTGTGS
APVVAEIAREMGILTVAIVTRPFKFEGPKRMRVAEEGMSKLADRVDGMIVVNNEKLLTA
VDKKVSFREAFLIADRVLYYGVKGISDVINVEGMINLDFADVRNLLANSGTVLMGIGAG
RGDKMAEEAAMSAIHSPLLERGIEGARRILVNVTGGYDLSMTDANEIVEKIREATGFDD
PDILFGITPDEAAGDEVRVTVIATGFGDNTYAAPLGGVVSSGRGGYDQGGYGSGLVRPV
RGGQGGLGGGSSYDPKDYDIPAFLRNVGGQ"
CDS 1972..2016
/codon_start=1
/label=linker0
/translation="GGGGSPAPAPGGGGS"
CDS 2017..2760
/codon_start=1
/label=mBiyu
/translation="VSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHGD
ANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQERT
ITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPDV
ENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKETR
DHMNVVEVFRAIDCKTAYAGMDELYK"
misc_feature 2770..2794
/label=Ptufb（RBS）
CDS 2795..3418
/locus_tag="TetR tn10"
/label=TetR tn10
misc_feature 3463..3498
/label=Term116 terminator
unsure 3499..3899
/label=ATrichBox
CDS complement(3997..5190)
/codon_start=1
/label=RepU
/translation="LRHNPFMVRLLEAGLISQISLQEGNHAPKPALTREISSSARSISP
PPVPTIAQQSQPAPAPLTIATRAELVQTLTACLDDAPCRESARRIFRALFSVALDVARA
CGYAEHVTRAVFHLPAELLMVHVGLKKSAFYENLQYLRRVGLVACDAHMGDLRGESVAT
GTLWAVTLKPRRVLEGKAGYVRLMHDDWGRNWRDLNADAKAGRTVYNMLHPLPKSVPES
QEPCEDVTGGELREKSAPVAVTYEVVSTWAIKSALPSPSDNMTVRPAPSLAENVVWELA
DAHRETRPAYRAEIVDRQARALAAAFGDGADSLGFWRKLIWNITRAADAGRDVSDDVGA
VLVRVLRDVKHDQTAGGTPPRNLAAVVNAALADLLGRLREYEGQRVGVRPDQAQFAA"
rep_origin 4403..4411
/label=DrOri2
rep_origin 4431..4439
/label=DrOri1
RBS 5199..5203
/label=RBS-repU
misc_feature 5205..5394
/label=RepU binding site
promoter 5215..5285
/label=Promoter?
misc_feature 5410..5645
/label=pGroES
misc_feature 5527..5576
/label=pGROES
misc_feature 5532..5537
/label=-35
misc_feature 5553..5561
/label=-10
primer_bind 5626..5668
/label=A465
primer_bind complement(5629..5650)
/label=A459
misc_feature 5631..5639
/label=rbs
primer_bind 5644..5668
/label=A423
misc_feature 5646..6461
/label=Kan
misc_feature 5985..5995
/label=Ecol
ORIGIN
1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgcta
721 agcttctgca gacgcgtcat ctgcagaatt cggcttccct atcagtgata gagattgaca
781 tccctatcag tgatagagat actgagcaca tcagcaggac gcactgaccg aattcattaa
841 agaggagaaa ggtaccgcat gcaagcagcc agaattcgcg tcatcggctt gggcggggcg
901 ggcaacaacg ccgtcaaccg catgatcgaa tcgggactcg agggcgtcga gttcatcgcc
961 ggcaacaccg acgcgcaggt gctcgccaag agccacgccg aagtgcgcat tcagctcggc
1021 gaccgcctga cgcgcggcct cggcgcgggc gcagacccca aggtgggcga agaagccgcc
1081 gtcgaagacc gcgaccgcat caaggaatat ctcgacgata ccgacatgct cttcatcacg
1141 gcgggcatgg gcggcggcac cggcaccggc tcggcgcccg tcgtggccga aattgcccgc
1201 gaaatgggca tcctgacggt tgctatcgtg acccgcccct tcaagttcga aggccccaag
1261 cgcatgcgcg tggccgaaga aggcatgagc aagctcgccg accgggtgga cggcatgatc
1321 gtggtgaaca acgaaaagct gctcaccgcc gtggacaaga aggtgtcgtt ccgcgaagcc
1381 ttcctgatcg cggaccgggt gctgtactac ggcgtcaagg gcatcagcga cgtgatcaac
1441 gtcgagggga tgatcaacct cgacttcgcc gacgtgcgca acctgctcgc caactccggc
1501 accgtcctga tgggcatcgg cgcggggcgc ggcgacaaga tggccgagga agctgccatg
1561 agcgccattc actcgccgct gctcgaacgc ggcattgagg gcgcgcgccg catcctggtc
1621 aacgtgaccg gcggctacga cctgtccatg accgatgcca acgagatcgt cgagaagatt
1681 cgcgaggcca ccggcttcga tgaccccgac atcctcttcg gcattacccc cgacgaggcg
1741 gcgggcgacg aagtgcgcgt gaccgtcatc gccaccggct tcggcgacaa cacctacgcg
1801 gctccgctgg gcggcgtggt cagcagcgga cgcggcggct acgaccaggg cggctacggc
1861 agcggtctgg tgcgcccggt gcgcggcggg cagggcggtc tgggtggcgg cagcagctac
1921 gaccccaagg actacgacat ccccgctttc ctgcgcaacg tcggcggaca gggtggcggt
1981 ggtagcccgg caccggcacc gggcggtggc ggtagcgtga gcaagggcga ggaggacaac
2041 atggccagct acggcgccct gctgttccgc gagaagatcc cctacgtggt ggagatggag
2101 ggcgacgtgg agggcatgaa gttcagcgtg cgcggcaagg gccacggcga cgccaacacc
2161 ggcaagatcg aggccagctt catctgcacc accggcgagc tgcccgtgcc ctggagcagc
2221 atcctgacca ccgtgaccta cggcgcccag tgcttcgcca agtaccccaa cgacatcaag
2281 gactacctga agagcaccat gcccgagggc tacgtgcagg agcgcaccat caccttcgag
2341 aacgacggca tctacaagac ccgcgccgag gtgacctacg agaagggcag cgtgtacaac
2401 cgcgtgaccc tgaacggcag cggcttcaag aagggcggca acatcctggg caagaagctg
2461 gagttcaact tcaacgccag ccgcacctac gtgctgcccg acgtggagaa caacggcatc
2521 aagtgctaca tcaacatccg ccacaacgtg atcggcggcg gccagatccg cgccgagcac
2581 cagcagctga acacccccct gggcggcggc cccgtggaca tcccccacta ccacgccatc
2641 cagacccaca ccatcctgag caaggacccc aaggagaccc gcgaccacat gaacgtggtg
2701 gaggtgttcc gcgccatcga ctgcaagacc gcctacgccg gcatggacga gctgtacaag
2761 taatctagcg ggcaaaaccc aggaggaaag actcatgtct agattagata aaagtaaagt
2821 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
2881 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
2941 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
3001 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
3061 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
3121 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
3181 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
3241 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
3301 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
3361 ccttgaattg atcatctgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaata
3421 actgcagccc gggggatccc atggtacgcg tgctagaggc ataaaaaatc cccccggtgg
3481 caatccgggg ggttttttcg cttctctatt tcaatatcta aaaattcttc actcttatcg
3541 ttgcttgaca tattaaaact gtctaactga tccagttcac ttatatggaa agtatcacta
3601 aagtcatggc ttctcagaaa ttcaatactt cccattgatg gggaccggtt aaccaaatcg
3661 ataaaaattt ttctgtcatg tttcagataa gtttttggcg cgttgatttt ataataaagc
3721 cgccattctt gtgccaacaa cgccacgaaa agagccacag cagcagcggc cacatcccat
3781 tgaaaatctt tcgccagacc aaccgccagg agaaccaagc cgaaatagtt agcccagcga
3841 actaaaaaac tatctataaa cgtattaaga aacgttcggg tcatatttcc gtgaatcata
3901 tcaggacgta aatttgcaca tgacgcattt ccgggggctt ttatccctcg gacttctttt
3961 caggcccctt ctcggccttt ctgtgaccct gttttttcag gccgcgaact gcgcctggtc
4021 gggcctgacc cccacacgct ggccttcgta ctcacgcagc ctgcccagca ggtcggccag
4081 cgcggcattg accacggcgg cgaggttgcg gggcggcgtt ccgcctgcgg tctggtcgtg
4141 cttcacgtcg cgcagcacgc gcaccagcac cgcgcccacg tcgtcggaca cgtcccggcc
4201 agcgtcggcg gcgcgggtga tgttccaaat gagtttccgc cagaagccca gcgagtcggc
4261 cccgtccccg aaagctgccg ctagcgcccg cgcctgcctg tccacgatct cggcgcggta
4321 ggccgggcgg gtttcccggt gggcgtcggc aagctcccac acgacgtttt cagcgaggga
4381 gggcgcgggc cggacagtca tgttatcgga gggggaggga agagcggatt ttatcgccca
4441 ggtgctcaca acttcgtatg tcacggcaac cggggcgctt ttttcgcgca gttccccgcc
4501 tgtcacatcc tcacagggtt cttgactctc cggcacgctc ttagggagag ggtggagcat
4561 gttgtagacg gtgcgcccgg ccttcgcgtc ggcgttcagg tcgcgccagt tccggcccca
4621 atcgtcatgc atcaggcgga catatcccgc cttcccttcc agcacgcgcc tcggcttgag
4681 cgtcacggcc cacagcgtcc cggttgccac agactcgccg cgcaggtcgc ccatgtgggc
4741 atcacaggcc accagcccga cccggcgcag atattgaagg ttctcgtaga aggcggactt
4801 cttcagaccc acatgcacca tgagcagctc ggcgggcagg tggaacacag cgcgggtgac
4861 gtgttccgca tagccgcagg cgcgggccac gtccagcgcc accgagaaca gggcgcggaa
4921 gatgcgccgg gccgactcgc ggcagggggc gtcatctagg caggcggtga gggtctgcac
4981 cagttcggcg cgtgtggcga tggtcaacgg cgcgggcgcg ggctgggact gctgggcgat
5041 ggtgggcacg ggcggcggcg agatggaccg ggccgagctg gaaatttccc gtgtgagcgc
5101 aggtttaggc gcatggttac cttcctgcag tgaaatctgc gaaatcaggc ccgcttcgag
5161 gagtcggacc atgaatggat tgtgtctcaa gaaaaaagcc tcccctcttg tcagagggga
5221 gggtcccggt ctaccatgct ctcagcgacg agatagctgg ggtttgtgac cctgcactct
5281 gacaaagccc tcaccgaaag gtgggggttt agtcatttgg cctagtaaag cagaacagag
5341 gcgatttgtc acgcaaaggc ccgccgcccg aggggaaaca ggcggcgggc caatggctcg
5401 gcgttgttat ggaagcacgt attgtcgccc tacatatata cgttaaagct aacagctggc
5461 aaggggatac ccccattccc cgtcccagcg ccccttgagc gtcatagact cagattgtca
5521 gcttcggtca gttgacattt ttcttatcgg cgctctacca tccgtgacgg attgaaggcg
5581 ctgggcggga aaaagctcgc cggcacgact ctccgccatt ccatctcact cacaggagga
5641 ccccaatgag ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccaaca
5701 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
5761 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
5821 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
5881 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
5941 ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
6001 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
6061 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
6121 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
6181 ggaaagaagt gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
6241 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
6301 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
6361 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
6421 ataaattgca gtttcatttg atgctcgatg agtttttcta gctgtcagac caagtttact
6481 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
6541 tcctttttga taatc
//
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