| 质粒名称: | PCMV-EGFP-LplA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000782 |
| 产品用途: | Mammalian PhCMV-driven constitutive EGFP-LplA expression vector (PhCMV-EGFP-LplA-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: |
EGFP-LplA
|
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
PCMV-EGFP-LplA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
PCMV-EGFP-LplA载体质粒基本信息
PCMV-EGFP-LplA质粒图谱载体图谱和PCMV-EGFP-LplA载体序列质粒序列多克隆位点信息
PCMV-EGFP-LplA质粒载体简介
PCMV-EGFP-LplA质粒序列载体序列
LOCUS PCMV-EGFP-LplA 5721 bp DNA circular SYN 11-FEB-2025
DEFINITION Localization: C1 Cloning Vector, Excitation: 488, Emission: 507.
ACCESSION .
VERSION .
KEYWORDS mEGFP-LplA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5721)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 5721)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5721
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 142..445
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 446..649
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 600..620
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 688..697
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 694..1410
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind 694..711
/label=oYK01-l-F
primer_bind complement(739..760)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1000..1019)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1347..1368
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 1441..2466
/label=LPLA
/note="LplA"
primer_bind complement(2447..2462)
/label=oYK01-1-R
polyA_signal 2590..2711
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2627..2646)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2681..2700
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2718..3173)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2855..2876)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3067..3086
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3200..3304
/gene="bla"
/label=AmpR promoter
promoter 3306..3663
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3329..3349)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3514..3649
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3576..3595
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3599..3610)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3698..4492
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3752..3771)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4362..4381
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4680..4699)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4724..4771
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5100..5688
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5589..5608
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
61 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
421 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
481 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
541 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
601 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
661 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
721 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
781 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
841 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
901 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
961 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
1021 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
1081 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
1141 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
1201 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
1261 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
1321 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
1381 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcc
1441 atgggctcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
1501 gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc
1561 aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
1621 cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc
1681 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaact
1741 atctccacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc cgaagcgtcc
1801 ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc gcaaagtctc aggctcggcc
1861 tatcgcgaaa ccaaagatcg cggcttccac cacggcacct tgctactcaa tgccgacctc
1921 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacg
1981 tcggtacgtt cccgcgtgac caacctcacc gagctgttgc cggggatcac ccatgagcag
2041 gtttgcgagg ccataaccga ggcctttttc gcccattatg gcgagcgcgt ggaagcggaa
2101 atcatctccc cgaacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgccag
2161 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
2221 tttacctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
2281 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg acgactgcaa
2341 ggctgcctgt accgcgcaga tatgctgcaa caggagtgcg aagcgctgtt ggttgacttc
2401 ccggaacagg aaaaagagct acgggagtta tcggcatgga tggcgggggc tgtaaggtag
2461 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
2521 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
2581 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
2641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
2701 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
2761 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
2821 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
2881 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
2941 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
3001 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
3061 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
3121 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
3181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
3241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
3301 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
3361 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
3421 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
3481 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
3541 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
3601 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
3661 aaagattgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
3721 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
3781 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
3841 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
3901 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
3961 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
4021 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
4081 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
4141 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
4201 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
4261 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
4321 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
4381 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
4441 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
4501 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
4561 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
4621 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
4681 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
4741 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
4801 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
4861 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
4921 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
4981 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
5041 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
5101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
5161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
5221 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
5281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
5341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
5401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
5461 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
5521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
5581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
5641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
5701 gcggcctttt tacggttcct g
//
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