pAAV-hSyn-DIO-NES-FRCaMPi-WPRE-bGH polyA

LOCUS       pAAV-hSyn-DIO-NES-FRCaMPi-WPRE-bGH polyA            6041 bp DNA     circular SYN 26-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        165..612
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    613..618
                     /label=SalI
     protein_bind    625..658
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    709..742
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    751..755
                     /label=KpnI
     CDS             complement(757..2073)
                     /codon_start=1
                     /label=NES-FRCaMPi
                     /translation="MLQLPPLERLTLSRSMVSKGEEDNMAIIKEFMRFKVHMEGSVNGH
                     EFEIEGEGEGRPYEAFQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFK
                     LSFPEGFRWERVMSFEDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGW
                     EASYEMTTRNLTDEQIAEFREAFSLLDRDQDGNITSNELGVVMRSLGLSPTAAELQDMI
                     NEVDADGNGTIDFTEFLTMMARKMKDTDDEEEVREAFKVFDKDGSGYITVEELTHVLTS
                     LGERLSREEVADVIREADSDGDGVINYEEFSRVISSKGGSGGSGSGSMRKPFRGAGNAV
                     RAFSTWKKLVPRVSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLPGA
                     YIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
     misc_feature    2079..2083
                     /label=NheI
     protein_bind    complement(2084..2117)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    complement(2168..2201)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2202..2207
                     /label=EcoRI
     misc_feature    2208..2796
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    2797..2802
                     /label=ClaI
     polyA_signal    2805..3281
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     misc_feature    3282..3287
                     /label=PmlI
     repeat_region   3304..3433
                     /label=AAV2 ITR
     rep_origin      3519..3974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4256..4360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4361..5221
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5392..5980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagaagtgca agtgggtttt
      181 aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac
      241 aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac
      301 aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc
      361 ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc
      421 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
      481 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
      541 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
      601 ctgagagcgc aggtcgactc cggaataact tcgtatagga tactttatac gaagttatgc
      661 agaatggtag ctggattgta gctgctatta gcaatatgaa acctcttaat aacttcgtat
      721 agcatacatt atacgaagtt atggcgcggg ggtaccctac ttgtacagcg cgtccgtgcc
      781 accggtgggg tggcggccca cggcgcgttc gcactgttcc acgatggtgt agtcctcgtt
      841 gtgggacaag atgtccaact cgatgtcgac gatgtaggcg ccgggcagct gcacgggctt
      901 cttggccttg taggtggtct tgacctcggc ggcgtagtgg ccgccgtcct tcagcctcag
      961 ccccttcctg atctcgctct tcagggcgcc gtcctcgggg tacatccact cggaaacccg
     1021 cggcactagc tttttccaag tgctgaaagc acgcacagcg ttgcccgcgc cacggaacgg
     1081 tttcctcatt gaacctgacc cgctgcctcc tgaaccgccc ttggaagaaa tgacacgaga
     1141 aaattcttcg tagttgatta caccatcgcc atcggagtcg gcttcacgta tcacatcggc
     1201 tacttcttct cgagacaaac gttcaccgag acttgtaaga acatgagtca gctcctcgac
     1261 tgtaatgtat ccacttccat ctttatcgaa gactttaaag gcttcgcgaa cttcctcttc
     1321 gtcgtcggta tccttcattt tccgggccat cattgtcaaa aattcggtaa aatcaattgt
     1381 gccattacca tcggcatcga cctcattaat catatcttgt aattcggcgg cagtaggcga
     1441 tagacctaac gacctcataa ccacacccaa ttcattggac gtgatatttc catcctgatc
     1501 acgatcaagc agcgaaaagg cctcacggaa ctccgcaatc tgctcatctg taaggttacg
     1561 ggtagtcatc tcgtaggaag cctcccagcc catggtcttc ttctgcatta cggggccgtc
     1621 gggggggaag ttggtgccga gcagcttcac cttgtagatg aatacgccgt cctgcaggga
     1681 ggagtcctga ttaacgtgaa taatgccgcc gtcctcgaag ctcatcacgc gctcccacct
     1741 gaagccctcg gggaaggaca gcttgaagta gtcggggatg tcggctgggt gcttaatgta
     1801 ggccttggag ccgtacatga actgagggga caggatgtcc caggcgaagg gcagggggcc
     1861 acccttggtc accttcagcc tagcggtctg gaaggcctcg taggggcggc cctcgccctc
     1921 gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat
     1981 gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatag atctcgaaag
     2041 agtaagacgt tcaagaggag gaagttgaag catggtggct agcataactt cgtataaagt
     2101 atcctatacg aagttatttg ccttaaccca gaaattatca ctgttattct ttagaatggt
     2161 gcaaagaata acttcgtata atgtatgcta tacgaagtta tgaattcaat caacctctgg
     2221 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2281 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2341 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2401 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2461 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2521 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2581 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     2641 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2701 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     2761 agacgagtcg gatctccctt tgggccgcct ccccgcatcg atacgggtgg catccctgtg
     2821 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     2881 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     2941 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     3001 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     3061 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     3121 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     3181 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     3241 tgggattaca ggcgtgaacc actgctccct tccctgtcct tcacgtgcgg accgagcggc
     3301 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3361 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3421 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3481 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     3541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3661 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3721 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3841 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     3901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3961 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4021 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4081 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4141 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4201 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4261 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4321 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4381 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4441 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4501 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4561 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4621 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4681 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4741 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4801 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4861 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4921 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4981 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5041 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5101 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5161 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5221 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5281 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5521 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5761 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6001 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//