R4pGWB601_UBQ10p-miniTurbo-NES-YFP

LOCUS       R4pGWB601_UBQ10p-miniTurbo-NES-YFP               12692 bp ds-DNA     circular SYN 18-SEP-2019
DEFINITION  Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10
            promoter in plants.
ACCESSION   .
VERSION     .
KEYWORDS    R4pGWB601_UBQ10p-miniTurbo-NES-YFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12692)
  AUTHORS   Mair A., Xu S., Branon T.C., Ting A.Y., Bergmann D.C
  TITLE     Proximity labeling of protein complexes and cell type-specific 
            organellar proteomes in Arabidopsis enabled by TurboID
  JOURNAL   https://www.biorxiv.org/content/10.1101/629675v1
REFERENCE   2  (bases 1 to 12692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 18, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12692
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(80..96)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(80..96)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(93..115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    104..120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(128..158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    173..194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      230..482
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(642..1193)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1245..1424)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    1930..1954
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2475..3266
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3512..4100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4001..4020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4254..4271
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4286..4426
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4352..4371)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4512..4534
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4770..4964
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5030..6103)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6532..7161)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8462..8486
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8674..8696
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8688..8705
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8689..8705
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8730..8750
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10769..10793
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10807..10848
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             11632..11658
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             11689..12408
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11734..11755)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11995..12014)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12342..12363
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(12431..12455)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      12487..47
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattggttcc
       61 ggaaccaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      181 gctaactcac attaggctga attaggcgcg cctatttcta ttaattcccg atctagtaac
      241 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
      301 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
      361 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
      421 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
      481 tcggggaaat tcgagctggg gacgtcccca gctcggtacc ccctcctggg cacggtcgta
      541 cgcgatccgc cgcggttcca cctcaccatt gcgggtatgc cagttgagtt cgatgagttc
      601 ccgtaccagc tccaactcgg gcgatgccac gcgcactccg ttcagatctc ggtgacgggc
      661 aggaccggac ggggcggtac cggcaggctg aagtccagct gccagaaacc cacgtcatgc
      721 cagttcccgt gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc
      781 tcgtgcatgc gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag
      841 ccctgtgcct ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc
      901 tggtggcggg gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc
      961 ttccaggggc ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag
     1021 ggatagcgct cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg
     1081 gtacggaagt tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc
     1141 atgtccgcct cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catatcttat
     1201 tgccccccgg gatcagatct ggattgagag tgaatatgag actctaattg gataccgagg
     1261 ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga
     1321 ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa
     1381 ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
     1441 aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct
     1501 catgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     1561 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     1621 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     1681 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     1741 cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc
     1801 cctgagtgct tgcggcagcg tgaagctgat ccgtcgagat taatagaaat aaattcagcc
     1861 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag
     1921 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg
     1981 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag
     2041 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac
     2101 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg
     2161 taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag
     2221 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2281 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2341 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2401 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2461 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2521 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2581 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2641 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2701 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2761 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2821 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2881 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2941 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3001 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3061 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3121 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3181 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3241 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     3301 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     3361 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     3421 tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3481 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3541 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3601 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3661 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3721 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3781 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3841 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3901 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3961 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4021 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4081 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4141 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4201 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4261 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     4321 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4381 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     4441 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4501 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4561 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     4621 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     4681 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     4741 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     4801 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     4861 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     4921 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     4981 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     5041 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     5101 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     5161 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     5221 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     5281 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     5341 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     5401 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     5461 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     5521 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     5581 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     5641 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
     5701 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
     5761 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
     5821 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
     5881 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
     5941 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
     6001 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
     6061 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
     6121 gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc
     6181 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
     6241 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
     6301 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
     6361 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
     6421 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
     6481 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
     6541 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
     6601 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
     6661 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
     6721 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
     6781 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
     6841 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
     6901 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
     6961 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
     7021 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
     7081 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
     7141 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
     7201 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
     7261 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
     7321 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
     7381 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
     7441 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
     7501 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
     7561 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
     7621 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
     7681 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
     7741 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
     7801 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
     7861 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
     7921 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
     7981 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
     8041 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
     8101 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
     8161 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
     8221 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
     8281 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
     8341 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
     8401 ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta
     8461 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga
     8521 caatctgatc caagctcaag ctgctctagc attcgccatt caggctgcgc aactgttggg
     8581 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     8641 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     8701 ccagtgccaa gcttgtggat cccccatcac aactttgtat agaaaagttg gctccgaatt
     8761 cgcccttagt ctagctcaac agagctttta acccaaattg gtacaataga atacaacttt
     8821 agatcataat tctcaaaaga aagagattcc ttagctattc tatctgccac tccatttcct
     8881 tctcggcttg tatgcacaag cataaaatcc tcaaacttgc taagtagata ctttatgtct
     8941 tggataattg gattgagact tgacaagcat aactttcatg taaccaaaga cacaagttgc
     9001 tgagaatcca cctcaaaaat gatcttccta taattgaatc gggataatga cagcacagcc
     9061 catctaagag cctccacttc tacttccagc acgcttctta cttttaccac agctcttgca
     9121 cctaaccata acaccttccc tgtatgatcg cgaagcaccc accctaagcc acattttaat
     9181 ccttctgttg gccatgcccc atcaaagttg cacttaaccc aagattgtgg tggagcttcc
     9241 catgtttctc gtctgtcccg acggtgttgt ggttggtgct ttccttacat tctgagcctc
     9301 tttccttcta atccactcat ctgcatcttc ttgtgtcctt actaatacct cattggttcc
     9361 aaattccctc cctttaagca ccagctcgtt tctgttcttc cacagcctcc caagtatcca
     9421 agggactaaa gcctccacat tcttcagatc aggatattct tgtttaagat gttgaactct
     9481 atggaggttt gtatgaactg atgatctagg accggataag ttcccttctt catagcgaac
     9541 ttattcaaag aatgttttgt gtatcattct tgttacattg ttattaatga aaaaatatta
     9601 ttggtcattg gactgaacac gagtgttaaa tatggaccag gccccaaata agatccattg
     9661 atatatgaat taaataacaa gaataaatcg agtcaccaaa ccacttgcct tttttaacga
     9721 gacttgttca ccaacttgat acaaaagtca ttatcctatg caaatcaata atcatacaaa
     9781 aatatccaat aacactaaaa aattaaaaga aatggataat ttcacaatat gttatacgat
     9841 aaagaagtta cttttccaag aaattcactg attttataag cccacttgca ttagataaat
     9901 ggcaaaaaaa aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat
     9961 acgcttcaat gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata
    10021 tttaaaaaat aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa
    10081 cggctggatc ttatgacgac cgttagaaat tgtggttgtc gacgagtcag taataaacgg
    10141 cgtcaaagtg gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac
    10201 ttttcctcaa cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct
    10261 caatacacgt gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct
    10321 agtcgtcctc gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc
    10381 ttcacaattc agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc
    10441 gtgatcaagg taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt
    10501 cgatcccaat ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaagacga
    10561 ttttctgggt ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca
    10621 tccgatttgt tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct
    10681 atctagatct ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt
    10741 ttctgattaa cagaagggcg aattcgaccc aagtttgtac aaaaaagcag gctccgcggc
    10801 cgcatgggca agcccatccc caaccccctg ctgggcctgg acagcaccgc tagcatcccg
    10861 ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct
    10921 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
    10981 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
    11041 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
    11101 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
    11161 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
    11221 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
    11281 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
    11341 caggaagcag ggattaacct ggacaggaat actctggccg ctatgctgat ccgagagctg
    11401 cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgtc acggtgggag
    11461 aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc
    11521 gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc
    11581 aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaagctgca gctgcctccc
    11641 ctggagcgcc tgaccctgga cggaggcggt ggaagcggcg gaggttccat ggtgagcaag
    11701 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
    11761 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
    11821 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
    11881 ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
    11941 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
    12001 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
    12061 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
    12121 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
    12181 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
    12241 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
    12301 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
    12361 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg gcccaagggt
    12421 gggcgcgccg acccagcttt cttgtacaaa gtggttgata acagcgctta gagctcgaat
    12481 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
    12541 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
    12601 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
    12661 taatacgcga tagaaaacaa aatatagcgc gc
//