pCAMBIA1300-cLuc

LOCUS       pCAMBIA1300-cLuc       10966 bp DNA     circular SYN 28-JAN-2019
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2022 from SnapGene 6.0.2 to Vector NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|867801600|
COMMENT     VNTDBDATE|867801600|
COMMENT     VNTNAME|pCAMBIA1300-cLuc|
COMMENT     VNTAUTHORNAME|Triple Threat|
FEATURES             Location/Qualifiers
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    860..1294
                     /label=cLuc
     misc_feature    1344..1418
                     /label=MCS
     terminator      1419..2060
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    2287..2311
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3611..4240
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
     CDS             4674..5741
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
     rep_origin      5807..6001
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6345..6485
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6671..7259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7346..8140)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
     misc_feature    8565..8589
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8667..8841
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8881..9906)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
     promoter        complement(9974..10651)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataaag aagttcattt catttggaga
      841 gaacacgggg gacgagctca tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
      901 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
      961 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     1021 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     1081 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     1141 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     1201 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     1261 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtggg
     1321 aggtggctca tctggcggag gtcagatctc gtacgcgtcc cggggcggta cccgggatcc
     1381 tctagagggc ccggcgcgcc actagtgtcg acctgcagag ctttcgttcg tatcatcggt
     1441 ttcgacaacg ttcgtcaagt tcaatgcatc agtttcattg cgcacacacc agaatcctac
     1501 tgagttcgag tattatggca ttgggaaaca tgtttttctt gtaccatttg ttgtgcttgt
     1561 aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag
     1621 aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt
     1681 ctcttatttg ttgtgtgttg aatttgaaaa tataagagat atgcaaacat tttgttttga
     1741 gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact
     1801 tgtagttgta ccattatgct tattcactag gcaacaaata tattttcaga cctagaaaag
     1861 ctgcaaatgt tactgaatac aagtatgtcc tcttgtgttt tagacattta tgaactttcc
     1921 tttatgtaat tttccagaat ccttgtcaga ttctaatcat tgctttataa ttatagttat
     1981 actcatggat ttgtagttga gtatgaaaat attttttaat gcattttatg acttgccaat
     2041 tgattgacaa catgcatcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     2101 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2161 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2221 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc
     2281 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     2341 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     2401 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     2461 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     2521 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     2581 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     2641 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     2701 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     2761 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     2821 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     2881 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     2941 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     3001 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     3061 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     3121 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     3181 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     3241 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     3301 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     3361 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     3421 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     3481 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     3541 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     3601 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     3661 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     3721 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     3781 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     3841 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     3901 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     3961 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     4021 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     4081 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     4141 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     4201 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     4261 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     4321 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     4381 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     4441 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     4501 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     4561 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
     4621 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
     4681 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     4741 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     4801 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     4861 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     4921 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     4981 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     5041 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     5101 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     5161 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     5221 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     5281 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     5341 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     5401 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     5461 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     5521 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     5581 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     5641 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     5701 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     5761 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     5821 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     5881 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     5941 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     6001 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     6061 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     6121 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     6181 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6241 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6301 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     6361 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     6421 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     6481 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7081 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7321 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta
     7381 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     7441 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     7501 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     7561 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     7621 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
     7681 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     7741 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     7801 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     7861 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     7921 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     7981 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     8041 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     8101 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
     8161 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     8221 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
     8281 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
     8341 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
     8401 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
     8461 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
     8521 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
     8581 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
     8641 tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg
     8701 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt
     8761 atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc tgggaactac
     8821 tcacacatta ttatggagaa actcgagctt gtcgatcgac agatccggtc ggcatctact
     8881 ctatttcttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     8941 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     9001 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     9061 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     9121 gagtcgtggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     9181 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     9241 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     9301 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     9361 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     9421 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     9481 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     9541 gatcgcatcc atagcctccg cgaccggttg tagaacagcg ggcagttcgg tttcaggcag
     9601 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa
     9661 ctccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     9721 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     9781 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     9841 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     9901 tttcatatct cattgccccc cgggatctgc gaaagctcga gagagataga tttgtagaga
     9961 gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag
    10021 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
    10081 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
    10141 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
    10201 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
    10261 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
    10321 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
    10381 gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
    10441 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
    10501 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
    10561 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
    10621 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
    10681 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
    10741 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    10801 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    10861 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    10921 agcggataac aatttcacac aggaaacagc tatgaccatg attacg
//