pAAVLINKv1-pCALM1-Cre-3'-KIF13B-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-KIF13B-FLAG               7171 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3884
                     /codon_start=1
                     /gene="KIF13B"
                     /gene_synonym="GAKIN"
                     /product="kinesin-like protein KIF13B"
                     /label=KIF13B
                     /note="kinesin 13B; guanylate kinase associated kinesin; 
                     kinesin-like protein GAKIN"
                     /db_xref="GI:46852172"
                     /db_xref="CCDS:CCDS55217.1"
                     /db_xref="GeneID:23303"
                     /db_xref="HGNC:HGNC:14405"
                     /db_xref="MIM:607350"
                     /protein_id="NP_056069.2"
                     /translation="GQSRRVQVEVKSVQESGTLPLMEECILSVGIGCVKVRPLRAPRTH
                     ETFHEEEEDMDSYQDRDLERLRRKWLNALTKRQEYLDQQLQKLVSKRDKTEDDADREAQ
                     LLEMRLTLTEERNAVMVPSAGSGIPGAPAEWTPVPGMETHIPVIFLDLNADDFSSQDNL
                     DDPEAGGWDATLTGEEEEEFFELQIVKQHDGEVKAEASWDSAVHGCPQLSRGTPVDERL
                     FLIVRVTVQLSHPADMQLVLRKRICVNVHGRQGFAQSLLKKMSHRSSIPGCGVTFEIVS
                     NIPEDAQGVEEREALARMAANVENPASADSEAYIEKYLRSVLAVENLLTLDRLRQEVAV
                     KEQLTGKGKLSRRSISSPNVNRLSGSRQDLIPSYSLGSNKGRWESQQDVSQTTVSRGIA
                     PAPALSVSPQNNHSPDPGLSNLAASYLNPVKSFVPQMPKLLKSLFPVRDEKRGKRPSPL
                     AHQPVPRIMVQSASPDIRVTRMEEAQPEMGPDVLVQTMGAPALKICDKPAKVPSPPPVI
                     AVTAVTPAPEAQDGPPSPLSEASSGYFSHSVSTATLSDALGPGLDAAAPPGSMPTAPEA
                     EPEAPISHPPPPTAVPAEEPPGPQQLVSPGRERPDLEAPAPGSPFRVRRVRASELRSFS
                     RMLAGDPGCSPGAEGNAPAPGAGGQALASDSEEADEVPEWLREGEFVTVGAHKTGVVRY
                     VGPADFQEGTWVGVELDLPSGKNDGSIGGKQYFRCNPGYGLLVRPSRVRRATGPVRRRS
                     TGLRLGAPEARRSATLSGSATNLASLTAALAKADRSHKNPENRKSWAS"
     misc_feature    2080..2085
                     /label=SDS
     misc_feature    2462..2467
                     /label=SDS
     misc_feature    2535..2540
                     /label=SDS
     misc_feature    2923..2928
                     /label=SDS
     CDS             3891..3956
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3960..4391
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4104..4109
                     /label=SDS
     repeat_region   4434..4574
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4434..4563
                     /label=AAV2 ITR
     repeat_region   4435..4575
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4649..5104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5386..5490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5491..6351
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6522..7110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggcagtcc cggagagttc
     1501 aagtcgaagt gaagtcagtg caggaatctg ggactttacc actgatggaa gaatgtatac
     1561 tgtctgttgg cattggatgt gtcaaagtta gaccgctcag agcccccaga acacatgaga
     1621 ccttccatga ggaagaggaa gacatggaca gctaccagga tcgagattta gagagacttc
     1681 gtagaaaatg gctaaatgca ttaacaaaac gtcaggagta cttggatcaa caattgcaaa
     1741 agcttgtcag taaacgtgat aaaacagagg atgatgctga ccgtgaagcg cagcttctgg
     1801 agatgcggtt gaccctaact gaggagagga acgcggtgat ggtcccctct gctggcagtg
     1861 gtattccagg ggccccagca gaatggaccc cagtacctgg gatggagaca cacattcctg
     1921 ttatattcct ggacttaaat gctgatgatt tcagctctca ggataatctt gatgacccag
     1981 aagctggtgg atgggatgcg accttgactg gggaagaaga agaggagttc tttgaattgc
     2041 agattgtgaa gcagcatgat ggggaggtga aagcagaagc ctcctgggac tccgcggtgc
     2101 atggctgccc tcagctcagc aggggcacgc ccgtggacga gcggttgttc ctgatcgtgc
     2161 gcgtgacggt ccagctcagc caccctgctg acatgcaact ggtgttacgc aagagaatct
     2221 gtgtcaatgt tcacggccgc cagggttttg cacagagtct cctaaaaaag atgtctcatc
     2281 gaagttctat tcctggctgt ggagtgactt ttgaaattgt ctccaatatt ccagaggatg
     2341 cccagggagt ggaagaacgg gaagcattag caagaatggc agccaatgtt gaaaacccag
     2401 cttctgctga ctcggaggct tatattgaaa agtacctcag gagcgtgctg gctgtagaaa
     2461 acctcctgac tttagatcgt ctgcgccagg aagttgcagt gaaggaacag ttaacaggaa
     2521 aaggaaagtt gagcaggagg agtatcagtt ctccaaatgt gaacagattg tctggaagcc
     2581 gacaagatct cattccatca tacagtctag gcagcaacaa gggccggtgg gaaagtcagc
     2641 aggatgtatc ccaaaccaca gtttccagag gaatagctcc tgcccccgcc ctctctgttt
     2701 ctccccaaaa taaccattct ccagatccag gactcagtaa ccttgcagca tcctacttga
     2761 atcctgtcaa atccttcgtg ccgcaaatgc caaagctcct caagtctctc tttcccgtcc
     2821 gcgatgagaa gaggggcaag cggccgtctc ccctcgcaca ccagcccgtg ccccgcatca
     2881 tggtgcagtc agccagcccg gacatcaggg tgaccaggat ggaggaggct cagccggaga
     2941 tgggccctga cgtgctggtg cagacgatgg gggccccggc cttgaagatc tgcgacaaac
     3001 ctgccaaagt gccttcccca ccgcctgtca tagctgtcac agcggtcacc ccggctccgg
     3061 aggcacagga cgggcccccc agccccctga gtgaagcctc tagcgggtac ttctcccaca
     3121 gcgtctccac cgcgaccctg tcggacgccc tgggccccgg cctggacgct gcggccccgc
     3181 cggggtccat gcccaccgcc cctgaggccg agcccgaggc gcccatcagc caccccccac
     3241 cgcccacggc cgtccccgcc gaggagcccc ctggccccca gcagctcgtg agccccggtc
     3301 gggagcgccc cgacctcgag gccccggcgc ccggctcccc gttccgcgtc cggagggtgc
     3361 gggcctcgga gttgcgctcc ttctcgcgca tgctggctgg ggaccccggc tgctccccgg
     3421 gggccgaggg gaatgcgccg gccccgggcg ccgggggaca ggccctggcc tctgattccg
     3481 aggaagctga cgaggtcccg gagtggctcc gagagggcga gttcgtcacc gtgggcgccc
     3541 acaaaacggg cgtggtgaga tacgtggggc ctgccgactt ccaagagggc acgtgggtcg
     3601 gcgtggagct cgacctgccc tcaggtaaga atgacggttc catcggcggg aagcagtact
     3661 tcaggtgtaa ccctggctac gggctgctgg tcaggcccag ccgggtccgc agggccacgg
     3721 gccctgtgcg gcggcgcagc acaggactcc ggctgggtgc ccccgaggcc cgccggagcg
     3781 ccaccctctc gggctccgcc accaacctgg cctcgctgac agctgccctg gccaaggccg
     3841 acaggagcca caagaaccct gagaaccgga aatcctgggc cagctgtaca gactacaaag
     3901 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaac
     3961 tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4021 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4081 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc
     4141 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4201 caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta
     4261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     4321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     4381 aggtttttta aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc
     4441 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4501 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4561 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4621 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4681 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     4741 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4801 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4861 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4921 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4981 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     5041 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5101 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5161 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5221 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5281 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5341 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5461 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5521 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5581 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5641 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5701 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5761 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5821 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5881 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5941 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6001 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6061 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6121 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6181 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6241 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6301 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6361 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6421 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6481 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6541 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6601 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6661 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6721 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6781 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6841 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6901 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6961 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7021 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7081 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7141 ctggcctttt gctggccttt tgctcacatg t
//