pAAVLINK-EF1alpha-HA-5'-NCOA1

LOCUS       pAAVLINK-EF1alpha-HA-5'-NCOA1             6366 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        146..688
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..3506
                     /codon_start=1
                     /gene="NCOA1"
                     /gene_synonym="bHLHe42; bHLHe74; F-SRC-1; KAT13A; 
                     RIP160;SRC1"
                     /product="nuclear receptor coactivator 1 isoform 1"
                     /EC_number="2.3.1.48"
                     /label=NCOA1
                     /note="isoform 1 is encoded by transcript variant 1;steroid
                     receptor coactivator-1; Hin-2 protein; renal carcinoma 
                     antigen NY-REN-52; class E basic helix-loop-helix protein 
                     74"
                     /db_xref="CCDS:CCDS1712.1"
                     /db_xref="GeneID:8648"
                     /db_xref="HGNC:HGNC:7668"
                     /db_xref="MIM:602691"
                     /protein_id="NP_003734.3"
                     /translation="SGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEEL
                     AELLSANISDIDSLSVKPDKCKILKKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQG
                     VIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENVTSYLGYNQEELMNTSVYSILHVGD
                     HAEFVKNLLPKSLVNGVPWPQEATRRNSHTFNCRMLIHPPDEPGTENQEACQRYEVMQC
                     FTVSQPKSIQEDGEDFQSCLICIARRLPRPPAITGVESFMTKQDTTGKIISIDTSSLRA
                     AGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAH
                     TKCKLCYPQSPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRVNPSVNPSISPAHGV
                     ARSSTLPPSNSNMVSTRINRQQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQ
                     ASSQSSNPSLNLNNSPMEGTGISLAQFMSPRRQVTSGLATRPRMPNNSFPPNISTLSSP
                     VGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPVLRQMSSQNSPSRLNIQP
                     AKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTSHKLVQL
                     LTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQ
                     EGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLRYLLD
                     KDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNTNPTPMTKPTPEEIKLEAQSQFTADLDQ
                     FDQLLPTLEKAAQLPGLCETDRMDGAVTSVTIKSEILPASLQSATARPTSRLNRLPELE
                     LEAIDNQFGQPGTGDQIPWTNNTVTAINQSKSEDQCISSQLDELLCPPTTVE"
     misc_feature    1176..1181
                     /label=SDS
     misc_feature    1710..1715
                     /label=SDS
     misc_feature    2258..2263
                     /label=SDS
     misc_feature    2749..2754
                     /label=SDS
     misc_feature    2817..2822
                     /label=SDS
     intron          3507..3547
                     /label=Splicing Donor
     misc_feature    3551..3584
                     /label=lox2272-JT1571
     repeat_region   3629..3769
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3629..3758
                     /label=AAV2 ITR
     repeat_region   3630..3770
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3844..4299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4581..4685
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4686..5546
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5717..6305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctagtg gcctcgggga cagttcatcc gaccctgcta acccagactc
      781 acataagagg aaaggatcgc catgtgacac actggcatca agcacggaaa agaggcgcag
      841 ggagcaagaa aataaatatt tagaagaact agctgagtta ctgtctgcca acattagtga
      901 cattgacagc ttgagtgtaa aaccagacaa atgcaagatt ttgaagaaaa cagtcgatca
      961 gatacagcta atgaagagaa tggaacaaga gaaatcaaca actgatgacg atgtacagaa
     1021 atcagacatc tcatcaagta gtcaaggagt gatagaaaag gaatccttgg gacctcttct
     1081 tttggaggct ttggatggat ttttctttgt tgtgaactgt gaagggagaa ttgtatttgt
     1141 gtcagagaat gtaaccagct acttaggtta caatcaggag gaattaatga ataccagcgt
     1201 ctacagcata ctgcacgtgg gggatcatgc agaatttgtg aagaatctgc taccaaaatc
     1261 actagtaaat ggagttcctt ggcctcaaga ggcaacacga cgaaatagcc atacctttaa
     1321 ctgcaggatg ctaattcacc ctccagatga gccagggacc gagaaccaag aagcttgcca
     1381 gcgttatgaa gtaatgcagt gtttcactgt gtcacagcca aaatcaattc aagaggatgg
     1441 agaagatttc cagtcatgtc tgatttgtat tgcacggcga ttacctcggc ctccagctat
     1501 tacgggtgta gaatccttta tgaccaagca agatactaca ggtaaaatca tctctattga
     1561 tactagttcc ctgagagctg ctggcagaac tggttgggaa gatttagtga ggaagtgcat
     1621 ttatgctttt ttccaacctc agggcagaga accatcttat gccagacagc tgttccaaga
     1681 agtgatgact cgtggcactg cctccagccc ctcctataga ttcatattga atgatgggac
     1741 aatgcttagc gcccacacca agtgtaaact ttgctaccct caaagtccag acatgcaacc
     1801 tttcatcatg ggaattcata tcatcgacag ggagcacagt gggctttctc ctcaagatga
     1861 cactaattct ggaatgtcaa ttccccgagt aaatccctcg gtcaatccta gtatctctcc
     1921 agctcatggt gtggctcgtt catccacatt gccaccatcc aacagcaaca tggtatccac
     1981 cagaataaac cgccagcaga gctcagacct tcatagcagc agtcatagta attctagcaa
     2041 cagccaagga agtttcggat gctcacccgg aagtcagatt gtagccaatg ttgccttaaa
     2101 ccaaggacag gccagttcac agagcagtaa tccctcttta aacctcaata attctcctat
     2161 ggaaggtaca ggaatatccc tagcacagtt catgtctcca aggagacagg ttacttctgg
     2221 attggcaaca aggcccagga tgccaaacaa ttcctttcct cctaatattt cgacattaag
     2281 ctctcccgtt ggcatgacaa gtagtgcctg taataataat aaccgatctt attcaaacat
     2341 cccagtaaca tctttacagg gtatgaatga aggacccaat aactccgttg gcttctctgc
     2401 cagttctcca gtcctcaggc agatgagctc acagaattca cctagcagat taaatataca
     2461 accagcaaaa gctgagtcca aagataacaa agagattgcc tcaattttaa atgaaatgat
     2521 tcaatctgac aacagctcta gtgatggcaa acctctggat tcagggcttc tgcataacaa
     2581 tgacagactt tcagatggag acagtaaata ctctcaaacc agtcacaaac tagtgcagct
     2641 tttgacaaca actgccgaac agcagttacg gcatgctgat atagacacaa gctgcaaaga
     2701 tgtcctgtct tgcacaggca cttccaactc tgcctctgct aactcttcag gaggttcttg
     2761 tccctcttct catagctcat tgacagaacg gcataaaatt ctacaccggc tcttacagga
     2821 gggtagcccc tcagatatca ccactttgtc tgtcgagcct gataaaaagg acagtgcatc
     2881 tacttctgtg tcagtgactg gacaggtaca aggaaactcc agtataaaac tagaactgga
     2941 tgcttcaaag aaaaaagaat caaaagacca tcagctccta cgctatcttt tagataaaga
     3001 tgagaaagat ttaagatcaa ctccaaacct gagcctggat gatgtaaagg tgaaagtgga
     3061 aaagaaagaa cagatggatc catgtaatac aaacccaacc ccaatgacca aacccactcc
     3121 tgaggaaata aaactggagg cccagagcca gtttacagct gaccttgacc agtttgatca
     3181 gttactgccc acgctggaga aggcagcaca gttgccaggc ttatgtgaga cagacaggat
     3241 ggatggtgcg gtcaccagtg taaccatcaa atcggagatc ctgccagctt cacttcagtc
     3301 cgccactgcc agacccactt ccaggctaaa tagattacct gagctggaat tggaagcaat
     3361 tgataaccaa tttggacaac caggaacagg cgatcagatt ccatggacaa ataatacagt
     3421 gacagctata aatcagagta aatcagaaga ccagtgtatt agctcacaat tagatgagct
     3481 tctctgtcca cccacaacag tagaaggtaa gtatcaaggt tacaagacag gtttaaggag
     3541 accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg
     3601 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     3661 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     3721 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     3781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     3841 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3901 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3961 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4021 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4081 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4141 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     4201 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     4261 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     4321 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4381 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4441 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4501 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4561 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4621 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4681 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4741 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4801 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4861 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4921 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4981 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5041 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5101 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5161 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5221 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5281 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5341 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5401 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5461 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5521 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5581 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5641 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5701 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5761 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5821 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5881 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5941 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6001 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6061 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6121 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6181 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6241 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6301 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6361 acatgt
//