| 质粒名称: | pMXs-Oct3/4 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13366 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pMXs |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | POU domain, class 5, transcription factor 1,Oct3/4 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1134 bp |
| 基因突变: | None |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pMX-S1811 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-Oct3/4 was a gift from Shinya Yamanaka (Addgene plasmid # 13366 ; http://n2t.net/addgene:13366 ; RRID:Addgene_13366) |
| 参考文献部分: | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174 |
买家导航
pMXs-Oct3/4载体 pMXsOct3/4质粒基本信息
pMXs-Oct3/4质粒图谱 pMXsOct3/4载体图谱和pMXs-Oct3/4载体序列 pMXsOct3/4质粒序列多克隆位点信息
pMXs-Oct3/4质粒 pMXsOct3/4载体简介
pMXs-Oct3/4质粒序列 pMXsOct3/4载体序列
LOCUS pMXs-Oct3/4 5959 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral expression of mouse Oct3/4.
ACCESSION .
VERSION .
KEYWORDS pMXs-Oct3/4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5959)
AUTHORS Takahashi K, Yamanaka S
TITLE Induction of pluripotent stem cells from mouse embryonic and adult
fibroblast cultures by defined factors.
JOURNAL Cell. 2006 Aug 25. 126(4):663-76.
PUBMED 16904174
REFERENCE 2 (bases 1 to 5959)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5959
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 120..589
/note="long terminal repeat from Moloney murine leukemia
virus"
protein_bind 906..930
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(2159..2183)
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 2234..2608
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind complement(2280..2296)
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
CDS complement(2618..3034)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(2634..2650)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(2666..2688)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 3093..3450
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
promoter 4061..4165
/gene="bla"
/label=AmpR promoter
CDS 4166..5026
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4384..4403)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5190..5778
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5679..5698
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5932..5949
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
901 atatcaccac tttgtacaag aaagctgggt tgatcaacag catcactgag cttctttccc
961 catcccaccc cccacccctg ttgtgctttt aatccctcct cagtaaaaga atttaacccc
1021 aaagctccag gttctcttgt ctacctccct tgccttggct cacagcatcc ccagggaggg
1081 ctggtgcctc agtttgaatg catgggagag cccagagcag tgacgggaac agagggaaag
1141 gcctcgccct caggaaaagg gactgagtag agtgtggtga agtgggggct tccatagcct
1201 ggggtgccaa agtggggacc tgggggcaga ggaaaggata cagccccccc tgggaaaggt
1261 gtccctgtag cctcatactc ttctcgttgg gaatactcaa tacttgatct tttgcccttc
1321 tggcgccggt tacagaacca tactcgaacc acatccttct ctagcccaag ctgattggcg
1381 atgtgagtga tctgctgtag ggagggcttc gggcacttca gaaacatggt ctccagactc
1441 cacctcacac ggttctcaat gctagttcgc tttctcttcc gggcctgcac cagggtctcc
1501 gatttgcata tctcctgaag gttctcattg ttgtcggctt cctccaccca cttctccagc
1561 aggggccgca gcttacacat gttcttaagg ctgagctgca aggcctcgaa gcgacagatg
1621 gtggtctggc tgaacacctt tccaaagaga acgcccaggg tgagccccac gtcggcctgg
1681 gtgtacccca aggtgatcct cttctgcttc agcagcttgg caaactgttc tagctccttc
1741 tgcagggctt tcatgtcctg ggactcctcg ggagttggtt ccaccttctc caacttcacg
1801 gcattggggc ggtcggcaca gggctcagag gaggttccct ctgagttgct ttccactcgt
1861 gctcctgcct ggccctcagg ctgcaaagtc tccacgccaa cttgggggac taggcccagt
1921 ccaacctgag gtccacagta tgccatccct ccgcagaact cgtatgcggg cggacatggg
1981 gagatcccca atacctctga gcctggtccg attccaggcc cacctggagg cccttggaag
2041 cttagccagg ttcgagaatc cacccagccc ggctccagcc ctgctgaccc atcaccccca
2101 cctggtgggg gtgagaaggc gaagtctgaa gccaggtgtc cagccatggg gaaggtggag
2161 cctgcttttt tgtacaaact tgtgatttcc cgtaccacca cactgggatc cttaattaac
2221 tagctagatc cggcagtcta gaggatggtc cacccccggg gtcggcagcc ttcacgtggg
2281 cggcgtgtat ccaagctgcg atgccgtcta ctttgagggc ggtgggggtg gtcagcagga
2341 ctgtgtaagg tcctttccag cgaggttcta ggttcttagt ctggtgtcgg cggacccaca
2401 ctgtgtcgcc gactcggtaa gggtgaggta ccaccggtcg gtccagttgt tcttggtagg
2461 ctgccgccag aggtctccag acttcgtgct ggactaagta gagagcctgt aagtgagctt
2521 ggagagaggg gctgttagta actcttgtca tgtcagggtc agggaagttt acaaggggcg
2581 ggggtgcccc atataagatc tcatatggcc atatgggggc gcctagagaa ggagtgaggg
2641 ctggataaag ggaggatcga ggcggggtcg aacgaggagg ttcaaggggg agagacgggg
2701 cggatggagg aagaggaggc ggaggcttag ggtgtacaaa gggcttgacc cagggagggg
2761 ggtcaaaagc caaggcttcc caggtcacga tgtaggggac ctggtctggg tgtccatgcg
2821 ggccaggtga aaagaccttg atcttaacct gggtgatgag gtctcggtta aaggtgccgt
2881 ctcgcggcca tccgacgtta aaggttggcc attctgcaga gcagaaggta acccaacgtc
2941 tcttcttgac atctaccgac tggttgtgag cgatccgctc gacatctttc cagtgaccta
3001 aggtcaaact taagggagtg gtaacagtct ggccctaatt ttcagacaaa tacagaaaca
3061 cagtcagaca gagacaacac agaacgatgc tgcagcagac aagacgcgcg gcgcggcttc
3121 ggtcccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc
3181 ctaccagaac cacatatccc tcctattagg ggggtgcacc aaagagtcca aaacgatcgg
3241 gatttttgga ctcaggtcgg gccacaaaaa cggcccccga agtccctggg acgtctccca
3301 gggttgcggc cgggtgttcc gaactcgtca gttccaccac gggtccgcca gatacagagc
3361 tagttagcta actagtaccg acgcaggcgc ataaaatcag tcatagacac tagacaatcg
3421 gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg tggtccctgg
3481 gcaggggtct cccgatcccg gacgagcccc caaatgaaag acccccgctg acgggtagtc
3541 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa
3601 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc
3661 gagtgagggg ttgtgggctc ttttattgag ctcggggagc agaagcgcgc gaacagaagc
3721 gagaagcgaa ctgattggtt agttcaaata aggcacaggg tcatttcagg tccttggggc
3781 accctggaaa catctgatgg ttctctagaa actgctgagg gctggaccgc atctggggac
3841 catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc
3901 catattcagc tgttccatct gttcctgacc ttgatctgaa cttttctatt ctcagttatg
3961 tatttttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg
4021 ggtctttcat tatgcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
4081 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
4141 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
4201 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
4261 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
4321 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
4381 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
4441 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
4501 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
4561 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
4621 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
4681 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
4741 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
4801 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
4861 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
4921 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
4981 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
5041 ttactcatat atactttaga ttgatttgcg gccggccgca aaaggatcta ggtgaagatc
5101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
5161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
5221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
5281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
5341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
5401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
5461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
5521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
5581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
5641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
5701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
5761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
5821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
5881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
5941 gtgagcgagg aagcggaag
//
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