| 质粒名称: | pHCMV-AmphoEnv |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 15799 |
| 存储实验室: | Miguel Sena-Esteves |
|
载体骨架基本信息 |
|
| 载体名称: | pHCMV |
| 载体出品公司: | Clontech |
| 空载体大小: | 4842 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 4070A Amphotropic Murine leukemia virus envelope gene |
| 物种来源: | Moloney murine leukemia virus |
| 插入大小: | 1962 bp |
| 基因突变: | -- |
| GenBank编号 | M33469 |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHCMV-AmphoEnv was a gift from Miguel Sena-Esteves (Addgene plasmid # 15799 ; http://n2t.net/addgene:15799 ; RRID:Addgene_15799) |
| 参考文献部分: | Optimized large-scale production of high titer lentivirus vector pseudotypes. Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW. J Virol Methods. 2004 Dec 15. 122(2):131-9. 10.1016/j.jviromet.2004.08.017 PubMed 15542136 |
买家导航
pHCMV-AmphoEnv载体 pHCMVAmphoEnv质粒基本信息
pHCMV-AmphoEnv质粒图谱 pHCMVAmphoEnv载体图谱和pHCMV-AmphoEnv载体序列 pHCMVAmphoEnv质粒序列多克隆位点信息
pHCMV-AmphoEnv质粒 pHCMVAmphoEnv载体简介
pHCMV-AmphoEnv质粒序列 pHCMVAmphoEnv载体序列
LOCUS pHCMV-AmphoEnv 6796 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pHCMV-AmphoEnv
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6796)
AUTHORS Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW
TITLE Optimized large-scale production of high titer lentivirus vector
pseudotypes.
JOURNAL J Virol Methods. 2004 Dec 15. 122(2):131-9.
PUBMED 15542136
REFERENCE 2 (bases 1 to 6796)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6796
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 97..476
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 477..680
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 631..651
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 677..701
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 792..1364
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
CDS 1441..3405
/codon_start=1
/gene="env"
/product="murine leukemia virus gPr80 envelope polyprotein
precursor"
/label=env
/note="proteolytically cleaved into gp70SU and p15TM
subunits"
/translation="MARSTLSKPPQDKINPWKPLIVMGVLLGVGMAESPHQVFNVTWRV
TNLMTGRTANATSLLGTVQDAFPKLYFDLCDLVGEEWDPSDQEPYVGYGCKYPAGRQRT
RTFDFYVCPGHTVKSGCGGPGEGYCGKWGCETTGQAYWKPTSSWDLISLKRGNTPWDTG
CSKVACGPCYDLSKVSNSFQGATRGGRCNPLVLEFTDAGKKANWDGPKSWGLRLYRTGT
DPITMFSLTRQVLNVGPRVPIGPNPVLPDQRLPSSPIEIVPAPQPPSPLNTSYPPSTTS
TPSTSPTSPSVPQPPPGTGDRLLALVKGAYQALNLTNPDKTQECWLCLVSGPPYYEGVA
VVGTYTNHSTAPANCTATSQHKLTLSEVTGQGLCMGAVPKTHQALCNTTQSAGSGSYYL
AAPAGTMWACSTGLTPCLSTTVLNLTTDYCVLVELWPRVIYHSPDYMYGQLEQRTKYKR
EPVSLTLALLLGGLTMGGIAAGIGTGTTALIKTQQFEQLHAAIQTDLNEVEKSITNLEK
SLTSLSEVVLQNRRGLDLLFLKEGGLCAALKEECCFYADHTGLVRDSMAKLRERLNQRQ
KLFETGQGWFEGLFNRSPWFTTLISTIMGPLIVLLLILLFGPCILNRLVQFVKDRISVV
QALVLTQQYHQLKPIEYEP"
RBS 2436..2444
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
primer_bind complement(3500..3519)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3565..3620
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3619..3638)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3978..3994)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3978..3994)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3991..4013)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4002..4018
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4026..4056)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4071..4092
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4209..4226)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4380..4968)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4460..4479)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5437..5816)
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
primer_bind 5763..5782
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6001..6105)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6131..6586)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6268..6289)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6480..6499
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6713..6735
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6727..6744
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6728..6744
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6754..6773
/label=T7
/note="T7 promoter, forward primer"
promoter 6754..6772
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
1381 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatct gggccgcacc
1441 atggcgcgtt caacgctctc aaaaccccct caagataaga ttaacccgtg gaagccctta
1501 atagtcatgg gagtcctgtt aggagtaggg atggcagaga gcccccatca ggtctttaat
1561 gtaacctgga gagtcaccaa cctgatgact gggcgtaccg ccaatgccac ctccctcctg
1621 ggaactgtac aagatgcctt cccaaaatta tattttgatc tatgtgatct ggtcggagag
1681 gagtgggacc cttcagacca ggaaccgtat gtcgggtatg gctgcaagta ccccgcaggg
1741 agacagcgga cccggacttt tgacttttac gtgtgccctg ggcataccgt aaagtcgggg
1801 tgtgggggac caggagaggg ctactgtggt aaatgggggt gtgaaaccac cggacaggct
1861 tactggaagc ccacatcatc gtgggaccta atctccctta agcgcggtaa caccccctgg
1921 gacacgggat gctctaaagt tgcctgtggc ccctgctacg acctctccaa agtatccaat
1981 tccttccaag gggctactcg agggggcaga tgcaaccctc tagtcctaga attcactgat
2041 gcaggaaaaa aggctaactg ggacgggccc aaatcgtggg gactgagact gtaccggaca
2101 ggaacagatc ctattaccat gttctccctg acccggcagg tccttaatgt gggaccccga
2161 gtccccatag ggcccaaccc agtattaccc gaccaaagac tcccttcctc accaatagag
2221 attgtaccgg ctccacagcc acctagcccc ctcaatacca gttacccccc ttccactacc
2281 agtacaccct caacctcccc tacaagtcca agtgtcccac agccaccccc aggaactgga
2341 gatagactac tagctctagt caaaggagcc tatcaggcgc ttaacctcac caatcccgac
2401 aagacccaag aatgttggct gtgcttagtg tcgggacctc cttattacga aggagtagcg
2461 gtcgtgggca cttataccaa tcattccacc gctccggcca actgtacggc cacttcccaa
2521 cataagctta ccctatctga agtgacagga cagggcctat gcatgggggc agtacctaaa
2581 actcaccagg ccttatgtaa caccacccaa agcgccggct caggatccta ctaccttgca
2641 gcacccgccg gaacaatgtg ggcttgcagc actggattga ctccctgctt gtccaccacg
2701 gtgctcaatc taaccacaga ttattgtgta ttagttgaac tctggcccag agtaatttac
2761 cactcccccg attatatgta tggtcagctt gaacagcgta ccaaatataa aagagagcca
2821 gtatcattga ccctggccct tctactagga ggattaacca tgggagggat tgcagctgga
2881 atagggacgg ggaccactgc cttaattaaa acccagcagt ttgagcagct tcatgccgct
2941 atccagacag acctcaacga agtcgaaaag tcaattacca acctagaaaa gtcactgacc
3001 tcgttgtctg aagtagtcct acagaaccgc agaggcctag atttgctatt cctaaaggag
3061 ggaggtctct gcgcagccct aaaagaagaa tgttgttttt atgcagacca cacggggcta
3121 gtgagagaca gcatggccaa attaagagaa aggcttaatc agagacaaaa actatttgag
3181 acaggccaag gatggttcga agggctgttt aatagatccc cctggtttac caccttaatc
3241 tccaccatca tgggacctct aatagtactc ttactgatct tactctttgg accttgcatt
3301 ctcaatcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc tctagttttg
3361 actcaacaat atcaccagct gaagcccata gagtacgagc catgataacc gtcgaggaat
3421 tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
3481 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
3541 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
3601 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
3661 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
3721 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
3781 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
3841 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
3901 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacggatcgg
3961 ccgcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
4021 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
4081 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
4141 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
4201 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
4261 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4801 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
4861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
4921 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
4981 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
5041 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5101 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
5161 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
5221 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
5281 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5341 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5401 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
5461 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5521 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg gttagctcct tcggtcctcc
5581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
5641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
5701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
5761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
5821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
5881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
5941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
6001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
6061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
6121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
6181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
6241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
6301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
6361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
6421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
6481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
6541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
6601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
6661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
6721 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
6781 gagctccacc gcggtg
//
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pHCMV-AmphoEnv质粒 pHCMVAmphoEnv载体应用举例
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