pSL1473

LOCUS      pSL1473           6443 bp DNA     circular SYN 11-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     123..164
                     /label=oSCL1800
     misc_feature    143..725
                     /label=CMV
     primer_bind     complement(699..730)
                     /label=OXB018
     primer_bind     706..749
                     /label=oSCL1791
     primer_bind     709..729
                     /label=oSCL1013
     misc_feature    731..732
                     /label=linker
     primer_bind     complement(734..748)
                     /label=Oscl28-2
     primer_bind     complement(735..748)
                     /label=8x R
     misc_feature    749..761
                     /label=linker
     misc_feature    767..785
                     /label=MS2 box
     misc_feature    791..800
                     /label=linker
     misc_feature    806..824
                     /label=MS2 box
     misc_feature    830..839
                     /label=linker
     misc_feature    830..839
                     /label=linker
     misc_feature    838..868
                     /label=hairpin
     misc_feature    844..862
                     /label=MS2 box
     misc_feature    867..879
                     /label=linker
     misc_feature    878..908
                     /label=hairpin
     misc_feature    884..902
                     /label=MS2 box
     misc_feature    912..917
                     /label=linker
     misc_feature    926..941
                     /label=linker
     primer_bind     927..943
                     /label=oSCL2471
     misc_feature    968..980
                     /label=linker
     misc_feature    986..1004
                     /label=MS2 box
     misc_feature    1010..1019
                     /label=linker
     misc_feature    1025..1043
                     /label=MS2 box
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1057..1087
                     /label=hairpin
     misc_feature    1063..1081
                     /label=MS2 box
     misc_feature    1086..1098
                     /label=linker
     misc_feature    1097..1127
                     /label=hairpin
     misc_feature    1103..1121
                     /label=MS2 box
     misc_feature    1131..1136
                     /label=linker
     misc_feature    1145..1160
                     /label=linker
     primer_bind     1146..1198
                     /label=oSCL2471
     primer_bind     1171..1193
                     /label=oSCL1092
     primer_bind     1172..1198
                     /label=OGT132-1-F
     primer_bind     1175..1197
                     /label=OGT145-F
     primer_bind     1175..1193
                     /label=oSCL1992
     misc_feature    1181..2086
                     /label=GLP1-Fc
     misc_feature    1322..1414
                     /label=GLP-1
     CDS             1367..1369
                     /codon_start=1
                     /label=G-E
                     /translation="G"
     CDS             1409..1411
                     /codon_start=1
                     /label=R-G
                     /translation="R"
     primer_bind     complement(2065..2087)
                     /label=oSCL2470
     primer_bind     complement(2065..2086)
                     /label=OGT132-1-R
     primer_bind     complement(2065..2086)
                     /label=OGT143-1-R
     primer_bind     complement(2067..2089)
                     /label=oSCL1093
     primer_bind     complement(2068..2114)
                     /label=oSCL2472
     primer_bind     complement(2068..2086)
                     /label=OGT145-R
     primer_bind     2087..2124
                     /label=oSCL1795
     polyA_signal    2096..2217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2096..2124
                     /label=OXB007
     primer_bind     complement(2107..2126)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2120..2140
                     /label=PSG5-R(SV40-R)
     primer_bind     2161..2180
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2188..2217)
                     /label=OXB016
     primer_bind     complement(2192..2217)
                     /label=OXB008
     primer_bind     complement(2201..2223)
                     /label=oSCL1801
     primer_bind     complement(2201..2217)
                     /label=oSCL1174
     primer_bind     complement(2201..2217)
                     /label=oSCL1796
     misc_feature    2222..2809
                     /label=CMV
     primer_bind     2222..2243
                     /label=oSCL1800
     primer_bind     complement(2778..2809)
                     /label=OXB018
     primer_bind     2785..2809
                     /label=oSCL1791
     primer_bind     2788..2808
                     /label=oSCL1013
     primer_bind     2810..2829
                     /label=T7
     primer_bind     2816..2865
                     /label=oSCL2413
     primer_bind     2839..2865
                     /label=oSCL2381
     CDS             2848..3000
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
                     CSVRQS"
     CDS             3001..3411
                     /codon_start=1
                     /label=LOV2
                     /translation="LERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRF
                     LQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIG
                     VQLDGTEHVRDAAEREGVMLIKKTAENIDEAAG"
     primer_bind     3005..3047
                     /label=oSCL2408
     primer_bind     complement(3005..3024)
                     /label=oSCL2407
     CDS             3025..3027
                     /codon_start=1
                     /label=V416I
                     /translation="V"
     primer_bind     3341..3383
                     /label=oSCL2410
     primer_bind     complement(3341..3360)
                     /label=oSCL2409
     CDS             3361..3363
                     /codon_start=1
                     /label=G528A
                     /translation="G"
     primer_bind     3371..3414
                     /label=oSCL2412
     primer_bind     complement(3371..3390)
                     /label=oSCL2411
     CDS             3391..3393
                     /codon_start=1
                     /label=N538E
                     /translation="N"
     CDS             3412..3639
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
                     SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
     primer_bind     complement(3620..3662)
                     /label=oSCL2415
     primer_bind     complement(3620..3642)
                     /label=oSCL2383
     primer_bind     complement(3620..3639)
                     /label=oSCL2382
     primer_bind     3620..3639
                     /label=oSCL2384
     misc_feature    3649..3697
                     /label=HSV TK poly(A) signal
     primer_bind     complement(3673..3705)
                     /label=oSCL1799
     repeat_region   3706..3846
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3706..3835
                     /label=AAV2 ITR
     rep_origin      3921..4376
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4008..4027)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4218..4239
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4393..4412)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4512..4534
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4572..4590)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4658..4762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4763..5623
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4981..5000)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5794..6382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6283..6302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gagttgacat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc cttgaaccgg taagaattgc aacaaacaaa tcaccaccgt gaggatcacc
      781 cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc
      841 tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac
      901 ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac tgatcggaga cgagtccgtc
      961 tcaaattgca acaaacaaat caccaccgtg aggatcaccc acggtggtga aagccttagc
     1021 ctagccgtga ggatcaccca cggtaggcac aagaaattct acccgtgagg atcacccacg
     1081 ggtagaatca caaagactac agccgtgagg atcacccacg gctgtagact caaacttaaa
     1141 aaggatatct tcagcaaact gaattcagct tagcgccacc atgaagatca tcctgtggct
     1201 gtgtgtgttc ggcctgttcc tggccaccct gttccccatc agctggcaga tgcccgtgga
     1261 gtccggcctg tcctccgagg actccgccag ctccgagagc ttcgccaagc gcatcaagcg
     1321 ccacggcgag ggcaccttca ccagcgacgt gagcagctac ctggagggcc aggccgccaa
     1381 ggagttcatc gcctggctgg tgaagggccg cggcagatct ggttgtaagc cttgcatatg
     1441 tacagtccca gaagtatcat ctgtcttcat cttcccccca aagcccaagg atgtgctcac
     1501 cattactctg actcctaagg tcacgtgtgt tgtggtagac atcagcaagg atgatcccga
     1561 ggtccagttc agctggtttg tagatgatgt ggaggtgcac acagctcaga cgcaaccccg
     1621 ggaggagcag ttcaacagca ctttccgctc agtcagtgaa cttcccatca tgcaccagga
     1681 ctggctcaat ggcaaggagt tcaaatgcag ggtcaacagt gcagctttcc ctgcccccat
     1741 cgagaaaacc atctccaaaa ccaaaggcag accgaaggct ccacaggtgt acaccattcc
     1801 acctcccaag gagcagatgg ccaaggataa agtcagtctg acctgcatga taacagactt
     1861 cttccctgaa gacattactg tggagtggca gtggaatggg cagccagcgg agaactacaa
     1921 gaacactcag cccatcatgg acacagatgg ctcttacttc gtctacagca agctcaatgt
     1981 gcagaagagc aactgggagg caggaaatac tttcacctgc tctgtgttac atgagggcct
     2041 gcacaaccac catactgaga agagcctctc ccactctcct ggtaaatgac tcgagtaaga
     2101 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2161 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttacg
     2221 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     2281 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2341 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2401 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2461 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2521 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2581 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2641 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2701 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2761 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct aatacgactc
     2821 actataggga agctttaagc caccatggct agcaacttca cacagttcgt gctggtggac
     2881 aacggcggca ccggcgacgt gaccgtggcc cctagcaact tcgccaacgg catcgccgag
     2941 tggatcagca gcaacagcag aagccaagcc tacaaggtga cctgcagcgt gagacagagc
     3001 ctggagagaa tcgagaagaa cttcgtgatc accgacccta gactgcccga caaccccatc
     3061 atcttcgcta gcgacagctt cctgcagctg accgagtaca gcagagagga gatcctgggc
     3121 agaaactgca gattcctgca aggccccgag accgacagag ccaccgtgag aaagatcaga
     3181 gacgccatcg acaatcagac cgaggtgacc gtgcagctga tcaactacac caagagcggc
     3241 aagaagttct ggaacctgtt ccacctgcag cccatgagag atcagaaggg cgacgtgcag
     3301 tacttcatcg gcgtgcagct ggacggcacc gagcacgtga gagacgccgc cgagagagag
     3361 ggcgtgatgc tgatcaagaa gaccgccgag aacatcgacg aggccgccgg gcagaagaga
     3421 aagtacacca tcaaagtcga ggtgcccaag gtggccaccc aaaccgtggg cggcgtggag
     3481 ctgcccgtgg ccgcctggag aagctacctg aacatggagc tgaccatccc catcttcgcc
     3541 accaacagcg actgcgagct gatcgtgaag gccatgcaag gcctgctgaa ggacggcaac
     3601 cccattccta gcgccatcgc tgctaacagc ggcatttatt aacaattgcg gcaataaaaa
     3661 gacagaataa aacgcacggg tgttgggtcg tttgttcgcg gccgcaggaa cccctagtga
     3721 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     3781 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     3841 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3901 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3961 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     4021 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4081 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     4141 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4201 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     4261 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     4321 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     4381 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     4441 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4501 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4561 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4621 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4981 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5461 tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag atggtaagcc
     5521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5941 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6181 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6361 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6421 ttgctggcct tttgctcaca tgt
//