pAAVLINKv2-SCP1-Cre-UDeg3a-ASH1L-C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-ASH1L-C-FLAG         6775 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3479
                     /codon_start=1
                     /gene="ASH1L"
                     /gene_synonym="ASH1; ASH1L1; KMT2H; MRD52"
                     /product="histone-lysine N-methyltransferase ASH1L 
                     isoform2"
                     /EC_number="2.1.1.359; 2.1.1.367"
                     /label=ASH1L
                     /note="isoform 2 is encoded by transcript variant 
                     2;probable histone-lysine N-methyltransferase ASH1L; 
                     ASH1-like protein; lysine N-methyltransferase 2H; absent 
                     small and homeotic disks protein 1 homolog; ash1 (absent, 
                     small, or homeotic)-like"
                     /db_xref="GI:110349788"
                     /db_xref="CCDS:CCDS1113.2"
                     /db_xref="GeneID:55870"
                     /db_xref="HGNC:HGNC:19088"
                     /db_xref="MIM:607999"
                     /protein_id="NP_060959.2"
                     /translation="RGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRN
                     WEKIRQKQEEVKHTSDNIHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRR
                     LAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKIS
                     DPLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSPVGRDVCRLRKAYYNAR
                     HEASAQIDEIVGETASEADSSETSVSEKENGHEKDDDVIRCICGLYKDEGLMIQCDKCM
                     VWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQ
                     GDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFR
                     PHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYIC
                     DYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKKDFSPHYVPDNYKRNGGRSSW
                     KSERSKPPLKDLGQEDDALPLIEEVLASQEQAANEIPSLEEPEREGATANVSEGEKKTE
                     ESSQEPQSTCTPEERRHNQRERLNQILLNLLEKIPGKNAIDVTYLLEEGSGRKLRRRTL
                     FIPENSFRK"
     misc_feature    1705..1710
                     /label=SDS
     misc_feature    3139..3144
                     /label=SDS
     CDS             3483..3548
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3564..3995
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3708..3713
                     /label=SDS
     repeat_region   4038..4178
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4038..4167
                     /label=AAV2 ITR
     repeat_region   4039..4179
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4253..4708
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4990..5094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5095..5955
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6126..6714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggaga ggccatctct
     1561 ctgaggaacc cagtgaaaat atcaacaccc caactagatt gaccccccaa ttacagatga
     1621 agccaatgtc caatcgtgaa aggaactttg tgttaaagca tcatgtattc ttggtccgaa
     1681 actgggagaa gattcgtcaa aaacaggagg aagtaaagca caccagtgat aatattcact
     1741 cagcatcatt atatacccgt tggaatggga tctgccgaga tgatgggaat atcaagtctg
     1801 atgtcttcat gacccagttc tctgccctgc agacagctcg atctgttcga acaagacggt
     1861 tggcagctgc agaggaaaat attgaagtgg ctcgggcagc ccgcctagcc cagatcttca
     1921 aagaaatttg tgatggtatc atctcttata aagattcttc ccggcaagca ctggcagctc
     1981 cacttttgaa ccttccccca aagaaaaaga atgctgatta ttatgagaag atctctgatc
     2041 ccctagatct tatcaccata gagaagcaga tcctcactgg ttactataag acagtggaag
     2101 cttttgatgc tgacatgctc aaagtctttc ggaatgctga gaagtactat gggcgtaaat
     2161 ccccagttgg gagagatgtt tgtcgtctac gaaaggccta ttacaatgcc cggcatgagg
     2221 catcagccca gattgatgag attgtgggag agacagcaag tgaggcagac agcagtgaga
     2281 cctcagtctc tgaaaaggag aatgggcatg agaaggacga cgatgttatt cgctgtatct
     2341 gtggcctcta caaggatgaa ggtctcatga tccagtgtga caagtgcatg gtatggcagc
     2401 actgtgattg tatgggagtg aactcagatg tggagcacta cctttgtgag cagtgtgacc
     2461 caaggcctgt ggacagggag gttcccatga tccctcggcc ccactatgcc caacctggct
     2521 gtgtctactt catctgtttg ctccgagatg acttgctgct tcgtcagggt gactgtgtgt
     2581 atctgatgag ggatagtcgg cgcacccctg atggccaccc ggtccgtcag tcctatcgac
     2641 tgttatctca cattaaccga gataaacttg acatctttcg cattgagaag ctttggaaga
     2701 atgaaaaaga ggaacggttt gcctttggtc accattattt ccgtccccac gaaacacacc
     2761 actctccatc ccgtcggttc tatcataatg aactatttcg ggtgccactc tatgagatca
     2821 ttcccttgga ggctgtagtg gggacctgct gtgtgttgga cctttatacg tattgtaaag
     2881 ggagacccaa aggagtaaag gagcaagatg tgtacatctg tgattatcgg cttgacaagt
     2941 cagcacacct gttttacaag atccaccgga accgctatcc tgtctgcacc aaaccctatg
     3001 cttttgatca cttccccaag aagctcactc ccaaaaaaga tttctcgcct cattacgtcc
     3061 cagacaacta caagaggaat ggaggacgat catcctggaa gtctgagcgc tcaaagccac
     3121 ccctaaaaga cttgggccag gaggatgatg ctctaccctt gattgaagag gttctagcca
     3181 gtcaagagca agcagccaat gagataccca gcctggagga gccagaacgg gaaggggcca
     3241 ctgctaacgt cagtgagggt gaaaaaaaaa cagaggaaag tagtcaagaa ccccagtcaa
     3301 cctgtacccc tgaggaacga cggcataacc aacgggaacg actcaaccag atcttgctca
     3361 atctccttga aaaaatccct ggaaaaaatg ccattgatgt gacctacttg ctggaggaag
     3421 gatcaggcag gaaactgcga aggcgtactt tgtttatccc agaaaacagc tttcgaaaga
     3481 aggactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
     3541 atgacaagta agaattctga tgactcgaga taatcaacct ctggattaca aaatttgtga
     3601 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3661 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3721 atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3781 aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc
     3841 tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat
     3901 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3961 ttcaggttca gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt
     4021 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     4081 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     4141 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     4201 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     4261 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4321 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4381 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4441 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     4501 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4561 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     4621 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4681 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     4741 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4801 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4861 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     4921 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4981 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5041 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5101 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5161 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5221 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5281 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5341 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5401 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5461 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5521 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5581 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5641 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5701 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5761 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5821 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5881 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5941 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6001 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6241 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6481 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//