| 质粒名称: | pAAVLINKv1-EF1alpha-Cre-3'-YE1-BE4max |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001914 |
| 产品用途: | 3' AAVLINK plasmid for YE1-BE4max expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | YE1-BE4max |
| 物种来源: | 未知 |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-EF1alpha-Cre-3'-YE1-BE4max
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-EF1alpha-Cre-3'-YE1-BE4max载体质粒基本信息
pAAVLINKv1-EF1alpha-Cre-3'-YE1-BE4max质粒图谱载体图谱和pAAVLINKv1-EF1alpha-Cre-3'-YE1-BE4max载体序列质粒序列多克隆位点信息
pAAVLINKv1-EF1alpha-Cre-3'-YE1-BE4max质粒载体简介
pAAVLINKv1-EF1alpha-Cre-3'-YE1-BE4max质粒序列载体序列
LOCUS pAAVLINKv1-EF1alpha-Cre-3'-YE1-BE4max 7139 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7139)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7139
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 714..1745
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1746..1786
/label=Splicing Donor
protein_bind 1791..1824
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1825..1916
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1917..3314
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="AERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDEND
KLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLES
EFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIET
NGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKK
DWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLE
AKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHY
EKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKP
IREQAENIIHLFTLTNLGAPAAFKYFNTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGD"
CDS 2664..3314
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII
KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQK
QLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFT
LTNLGAPAAFKYFNTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
CDS 3345..3593
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
misc_feature 3421..3426
/label=SDS
CDS 3624..3872
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 3915..3935
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 3939..4370
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4083..4088
/label=SDS
repeat_region 4413..4542
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
rep_origin complement(4604..5192)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5363..6223)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6224..6328)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6610..7065)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac aacgtacgcc accatgtcca
721 atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg
781 ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga
841 aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat
901 ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc
961 tggcagtaaa aaccatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg
1021 ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag
1081 aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg
1141 accaggttcg ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat
1201 ttctggggat tgcttataac accctgttac gtatagccga aattgccagg atcagggtta
1261 aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc
1321 tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc
1381 gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca
1441 gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag
1501 ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat
1561 acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg
1621 gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat attgtaatga
1681 actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg
1741 attaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagctt ataacttcgt
1801 ataggatact ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg
1861 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggccg
1921 agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa
1981 cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg
2041 acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt
2101 ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg
2161 cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc
2221 tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca
2281 agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga
2341 actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg
2401 agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc
2461 ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg
2521 gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga
2581 aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc
2641 tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc
2701 tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag
2761 ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt
2821 tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa
2881 acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga
2941 agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc
3001 actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg
3061 acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag
3121 agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct
3181 tcaagtactt taacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg
3241 acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc
3301 agctgggagg tgacagcggc gggagcggcg ggagcggggg gagcactaat ctgagcgaca
3361 tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg atgctgcctg
3421 aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg cacaccgcct
3481 acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc gagtataagc
3541 cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg ctgagcggag
3601 gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag gagacaggca
3661 agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa gaagtgatcg
3721 gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt accgacgaaa
3781 atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg gtcatccagg
3841 attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg
3901 gcagcgaatt cgagcccaag aagaagagga aagtctaact cgagataatc aacctctgga
3961 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
4021 tggatacgct gctttaatgc ctttgcatca tgctattgct tcccgtatgg ctttcatttt
4081 ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct
4141 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg
4201 tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg
4261 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
4321 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag
4381 accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
4441 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
4501 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggacatgtga gcaaaaggcc
4561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
4621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
4681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
4801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
4861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
4921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
4981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
5041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
5101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
5161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
5221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
5281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
5341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
5401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
5461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
5521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
5581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
5641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
5701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
5761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
5821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
5881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
5941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
6001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
6061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
6121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
6181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
6241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
6301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
6361 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
6421 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
6481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
6541 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
6601 tgcaccataa aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
6661 tcagctcatt ttttaaccaa tagaccgaaa tcggcaaaat cccttataaa tcaaaagaat
6721 agcccgagat agagttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
6781 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
6841 catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
6901 aagggagccc ccgatttaga gcttgacggg gaaagccggc gagcgtggcg agaaaggaag
6961 ggaagaaagc gaaaggagcg ggcgctaagg cgctggcaag tgtagcggtc acgctgcgcg
7021 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtactat ggttgctttg
7081 acgtatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcc
//
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