Lenti-mEGFP-AT5G43960

LOCUS       Lenti-mEGFP-AT5G43960             10294 bp DNA     circular SYN 11-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
FEATURES             Location/Qualifiers
     source          1..10294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    2202..2209
                     /label=enzyme cut site
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3509..4861
                     /codon_start=1
                     /label=AT5G43960
                     /translation="MATPYPGATQVGSYFVGQYYQVLQQQPDLIHQFYSEPSRAIRIDG
                     DSTETANSLLHIHNMVMSLNFTAIEVKTINSVESWEGGVLVVVSGSVKTKEFSNRRSFV
                     QTFFLAPQEKGYFVLSDVFLFVDEGTVYYHQPSYLSEIKHEAQLNPPTRHPDPQVSDYV
                     LEEEASDYVNAVQIKDDLVDKYSLQEDQHQPQHEDYEDEVAIEETPREEVAVDVVHEHR
                     AAPVEEPVGEKSKMSYASILKVAKEAATVPVAATQPSYNKSSQDINEWDQPMRTPSPQL
                     AAPLAPIQQSNSSTYVSDYGAEAEDGSGFEDFEFKSVYVRNLPSDISASEIEEEFKNFG
                     TIKPDGVFLRTRKDVMGVCYAFVEFEDMTSVENAIKASPIYLGGRQVYIEERRPNPAGV
                     RGARRGGGRGRGGYPTEAPRGRFGGRGSGRGNQDGGDYRPRGNGYYRGGGR"
     misc_feature    4862..4867
                     /label=enzyme cut site
     promoter        4872..5371
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4907..4930)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5304..5323
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5398..6192
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5452..5471)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6062..6081
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6216..6804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6269..6289)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6687..6698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7012..7645
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7774..7790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7774..7790)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7787..7809)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7798..7814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7822..7852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7867..7888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8005..8022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8176..8761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8256..8275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8932..9792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9555..9574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9793..9897)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9945..10079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9982..10001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10036..10055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     3481 cggactcaga tctcgagctc aagcttccat ggcgactcct tatcctggag cgacgcaggt
     3541 gggttcgtac tttgtggggc agtattatca agtgttgcag cagcagccag atcttattca
     3601 tcagttttat tctgagccta gtagagctat tcgtatcgat ggtgattcca ccgagactgc
     3661 taattctttg ctgcatattc ataacatggt tatgtcactg aatttcactg cgattgaagt
     3721 gaagacgatt aattcagtcg agtcgtggga aggtggtgtt cttgtggtgg tttcaggatc
     3781 tgtgaaaacc aaggaattta gcaacagaag gagttttgtg cagacctttt ttctcgctcc
     3841 tcaggaaaag ggttattttg ttctcagtga tgtatttctg tttgttgatg agggtactgt
     3901 ttactatcat cagccttctt atttatctga aatcaagcat gaggctcagc ttaatcctcc
     3961 tacccgtcat ccagatcccc aagtttctga ctatgtgctg gaggaagagg caagcgatta
     4021 tgtgaacgca gtccaaatca aagatgatct tgttgataag tacagtctgc aagaggacca
     4081 acatcaacct cagcatgaag actatgaaga tgaagttgcg attgaggaaa cacctaggga
     4141 agaagttgca gttgatgtgg tacatgaaca tcgggctgca ccagtagagg aaccagttgg
     4201 tgaaaaatca aagatgagtt atgcttccat tttaaaagtt gcaaaggaag cagcaactgt
     4261 acctgtagct gctacacaac catcatataa caagagctct caagatatta atgagtggga
     4321 tcaaccaatg cggactcctt cccctcagct ggctgcgcct cttgccccta ttcagcaatc
     4381 aaactcatcc acatatgttt ccgactatgg agcagaggca gaagatggct ccggttttga
     4441 agactttgag ttcaaatcag tgtacgttag gaatttgcct tccgacatat ctgcttctga
     4501 aatagaggaa gagtttaaga acttcggtac aatcaagcct gatggtgtgt tcctcagaac
     4561 ccgcaaggat gttatgggcg tatgttatgc atttgttgag tttgaggaca tgacttccgt
     4621 tgagaatgcg attaaggctt ctcctatata cttgggtgga aggcaagtat acattgagga
     4681 acgaagacca aatcccgctg gtgttcgcgg agcaagaaga ggaggaggac gtggaagggg
     4741 tggttaccca acagaagcac caagaggccg gtttggtggg cgtgggtctg gcagaggaaa
     4801 ccaggatgga ggtgactaca ggccaagagg aaatggttac taccgcggtg gtggtcgcta
     4861 agaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     4921 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     4981 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     5041 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     5101 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     5161 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     5221 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     5281 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     5341 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttcc taggaccatg
     5401 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5461 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5521 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     5581 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5641 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5701 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5761 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5821 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5881 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     5941 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6001 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6061 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6121 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6181 gagttcttct gacccgggcg gggcgcgtct ggaacaatca acctctggat tacaaaattt
     6241 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6301 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6361 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6421 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6481 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6541 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6601 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6661 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6721 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6781 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     6841 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     6901 acaagaggag gaggaggtgg gtttttccag tcacacctca ggtaccttta agaccaatga
     6961 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     7021 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7081 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     7141 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     7201 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7261 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     7321 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     7381 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     7441 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     7501 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7561 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7621 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7681 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc
     7741 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7801 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7861 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7921 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7981 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8581 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8701 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8761 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8821 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8881 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8941 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9001 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9061 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9121 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9181 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9241 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9301 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9361 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9421 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9481 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9541 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9601 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9661 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9721 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9781 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9901 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
     9961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    10081 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    10141 ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga attattttca
    10201 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt
    10261 gtatttgtta aatatgtact acaaacttag tagt
//