MMK

LOCUS       MMK             10701 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  a modified episomal (EBNA1/OriP) vector expressing human Myc and 
            KLF4 genes.
ACCESSION   .
VERSION     .
KEYWORDS    MMK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, 
            Cheng L
  TITLE     A Facile Method to Establish Human Induced Pluripotent Stem Cells 
            From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture 
            Conditions: A Clinically Compliant Approach.
  JOURNAL   Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
  PUBMED    25742692
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      2084..3873
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     4436..4458
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    4601..4735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4638..4657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4692..4711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             4832..4843
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5241..5261
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5331..5354)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5373..5402)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(6858..6917)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(6918..8234)
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     promoter        complement(8293..8700)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     rep_origin      complement(8883..9471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8963..8982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9642..10502)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10265..10284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10503..10607)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10675..10693
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat tctcatgttt gacagcttat catcgataag ctgatcctca caggccgcac
       61 ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg
      121 aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag gaatttgagg
      181 cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt tccgggctgc
      241 gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca gacctggatc
      301 tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg ggcaggagtc
      361 tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc tcttttagtg
      421 tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg agagaaggga
      481 gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg gggtgataac
      541 catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg agccccgggc
      601 ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa acgtccaagt
      661 tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc gggaggggca
      721 ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca
      781 ggaggggcag gagcaggagg aggggcagga gcaggagggg caggagcagg aggtggaggc
      841 cggggtcgag gaggcagtgg aggccggggt cgaggaggta gtggaggccg gggtcgagga
      901 ggtagtggag gccgccgggg tagaggacgt gaaagagcca gggggggaag tcgtgaaaga
      961 gccaggggga gaggtcgtgg acgtggagaa aagaggccca ggagtcccag tagtcagtca
     1021 tcatcatccg ggtctccacc gcgcaggccc cctccaggta gaaggccatt tttccaccct
     1081 gtaggggaag ccgattattt tgaataccac caagaaggtg gcccagatgg tgagcctgac
     1141 gtgcccccgg gagcgataga gcagggcccc gcagatgacc caggagaagg cccaagcact
     1201 ggaccccggg gtcagggtga tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat
     1261 cgtggtcaag gaggttccaa cccgaaattt gagaacattg cagaaggttt aagagctctc
     1321 ctggctagga gtcacgtaga aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc
     1381 gtatatggag gtagtaagac ctccctttac aacctaaggc gaggaactgc ccttgctatt
     1441 ccacaatgtc gtcttacacc attgagtcgt ctcccctttg gaatggcccc tggacccggc
     1501 ccacaacctg gcccgctaag ggagtccatt gtctgttatt tcatggtctt tttacaaact
     1561 catatatttg ctgaggtttt gaaggatgcg attaaggacc ttgttatgac aaagcccgct
     1621 cctacctgca atatcagggt gactgtgtgc agctttgacg atggagtaga tttgcctccc
     1681 tggtttccac ctatggtgga aggggctgcc gcggagggtg atgacggaga tgacggagat
     1741 gaaggaggtg atggagatga gggtgaggaa gggcaggagt gatgtaactt gttaggagac
     1801 gccctcaatc gtattaaaag ccgtgtattc ccccgcacta aagaataaat ccccagtaga
     1861 catcatgcgt gctgttggtg tatttctggc catctgtctt gtcaccattt tcgtcctccc
     1921 aacatggggc aattgggcat acccatgttg tcacgtcact cagctccgcg ctcaacacct
     1981 tctcgcgttg gaaaacatta gcgacattta cctggtgagc aatcagacat gcgacggctt
     2041 tagcctggcc tccttaaatt cacctaagaa tgggagcaac cagcaggaaa aggacaagca
     2101 gcgaaaattc acgccccctt gggaggtggc ggcatatgca aaggatagca ctcccactct
     2161 actactgggt atcatatgct gactgtatat gcatgaggat agcatatgct acccggatac
     2221 agattaggat agcatatact acccagatat agattaggat agcatatgct acccagatat
     2281 agattaggat agcctatgct acccagatat aaattaggat agcatatact acccagatat
     2341 agattaggat agcatatgct acccagatat agattaggat agcctatgct acccagatat
     2401 agattaggat agcatatgct acccagatat agattaggat agcatatgct atccagatat
     2461 ttgggtagta tatgctaccc agatataaat taggatagca tatactaccc taatctctat
     2521 taggatagca tatgctaccc ggatacagat taggatagca tatactaccc agatatagat
     2581 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat
     2641 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
     2701 taggatagcc tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg
     2761 gtagtatatg ctacccatgg caacattagc ccaccgtgct ctcagcgacc tcgtgaatat
     2821 gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg tgtgtaattt gtcctccaga
     2881 tcgcagcaat cgcgccccta tcttggcccg cccacctact tatgcaggta ttccccgggg
     2941 tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt ggttagcggg gttacaatca
     3001 gccaagttat tacaccctta ttttacagtc caaaaccgca gggcggcgtg tgggggctga
     3061 cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc acttgtcttt gtttatgggc
     3121 cccattggcg tggagccccg tttaattttc gggggtgtta gagacaacca gtggagtccg
     3181 ctgctgtcgg cgtccactct ctttcccctt gttacaaata gagtgtaaca acatggttca
     3241 cctgtcttgg tccctgcctg ggacacatct taataacccc agtatcatat tgcactagga
     3301 ttatgtgttg cccatagcca taaattcgtg tgagatggac atccagtctt tacggcttgt
     3361 ccccacccca tggatttcta ttgttaaaga tattcagaat gtttcattcc tacactagta
     3421 tttattgccc aaggggtttg tgagggttat attggtgtca tagcacaatg ccaccactga
     3481 accccccgtc caaattttat tctgggggcg tcacctgaaa ccttgttttc gagcacctca
     3541 catacacctt actgttcaca actcagcagt tattctatta gctaaacgaa ggagaatgaa
     3601 gaagcaggcg aagattcagg agagttcact gcccgctcct tgatcttcag ccactgccct
     3661 tgtgactaaa atggttcact accctcgtgg aatcctgacc ccatgtaaat aaaaccgtga
     3721 cagctcatgg ggtgggagat atcgctgttc cttaggaccc ttttactaac cctaattcga
     3781 tagcatatgc ttcccgttgg gtaacatatg ctattgaatt agggttagtc tggatagtat
     3841 atactactac ccgggaagca tatgctaccc gtttagggtt aacaaggggg ccttataaac
     3901 actattgcta atgccctctt gagggtccgc ttatcggtag ctacacaggc ccctctgatt
     3961 gacgttggtg tagcctcccg tagtcttcct gggcccctgg gaggtacatg tcccccagca
     4021 ttggtgtaag agcttcagcc aagagttaca cataaaggca atgttgtgtt gcagtccaca
     4081 gactgcaaag tctgctccag gatgaaagcc actcagtgtt ggcaaatgtg cacatccatt
     4141 tataaggatg tcaactacag tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca
     4201 tgtggaacag ggcccagttg gcaagttgta ccaaccaact gaagggatta catgcactgc
     4261 cccgcgaaga aggggcagag atgtcgtagt caggtttagt tcgtccgggg cggggatcga
     4321 tcctctagta gagtcgacct catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     4381 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4441 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gccggatcat
     4501 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4561 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4681 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgccc
     4741 aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg
     4801 gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg
     4861 acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct
     4921 tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg
     4981 caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg
     5041 aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag
     5101 tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc
     5161 aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag
     5221 gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca
     5281 atctttcaca aattttgtaa tccagaggtt gattgtcgac gcgtttaaac cttatcgtcg
     5341 tcatccttgt aatccaggat atcatttgct gccagatcct cttctgagat gagtttctgc
     5401 tcgagcggcc gcgtacgcgt aaaatgcctc ttcatgtgta aggcgaggtg gtccgacctg
     5461 gaaaatgctc ggtcgcattt ttggcactgg aacgggcggt gccccgtgtg tttacggtag
     5521 tgcctggtca gttcatctga gcgggcgaat ttccatccac agccgtccca gtcacagtgg
     5581 taaggtttct cacctgtgtg ggttcgcagg tgtgccttga gatgggaact ctttgtgtag
     5641 gttttgccgc agcccgcgta atcacaagtg tgggtggcgg tccttttccg gggccacgat
     5701 cgtcttcccc tctttggctt gggctcctct ggcatgcagg aaccgggtgg catgagctct
     5761 tggtaatgga gcggcgggac ttgcggctgc atctgatcgg gcaggaagga tgggtaattg
     5821 ggccccgggt ggggatggaa gccgggagga agcggcaggg cagggtgaca gtccctgctg
     5881 ctcagcactt cctcaagacc cagggtcggg gtagtcctgc tggggagctg ccgccccagg
     5941 gggaagtcgt gtgcagccgg ccggtggcca ttgctgagag ggggtccagc gcccaagtgg
     6001 gtgcacgaag agaccgcctc ctgcttgatc ttggggcacg tgcgcggcgg cccgccgttg
     6061 tagggcgcca ccaccaccgg gtggctgccg tcagggctgc ctttgctgac gctgatgacc
     6121 gacgggctgc cgtactcgct gccaggggcg ctcagcgacg ccttcagcac gaacttgccc
     6181 atcagcccgc cacctggcgg ctgcggctgc tgcggcggaa tgtacaccgg gtccaattct
     6241 ggccgcagga gctcggccac gaagccgccc gaggggctca cgtcgttgat gtccgccagg
     6301 ttgaagggag ccgtcggagg gggagcggac tccctgccat agaggaggcc tccgcccgtg
     6361 ccgcccggcg ccacgcccgg gtcgttcccg gcccggatcg gataggtgaa gctgcaggtg
     6421 gagggcgcgc tggcagggcc gctgctcgac ggcgacgacg aagaggaggc tgacgctgac
     6481 gaggacacgg tggcggccac tgactccgga ggatgggtca gcgaattgga gagaataaag
     6541 tccaggtcca ggagatcgtt gaactcctcg gtctctctcc gaggtagggg cgccaggttg
     6601 ctaccgccgc aagccgcacc ggctccgccg ctctccaggt ctgtggccac ggtcgccgcc
     6661 gccaggtcat aggggcggcc gggaagcact gggggaagtc gcttcatgtg ggagagctcc
     6721 tcccgccagc ggttattcgg ggcacctgct tgacgcagtg tcttctccct tcccgccggg
     6781 ccagacgcga acgtggagaa agatgggagc agcgcgtcgc tgacagccat gtcagactcg
     6841 ccaggtggct gcctcatagg accggggtta ctttcaacat cgccagcgag tttcaacaaa
     6901 gcgtagttag tacattgcgc acaagagttc cgtagctgtt caagtttgtg tttcaactgt
     6961 tctcgtcgtt tccgcaacaa gtcctcttca gaaatgagct tttgctcctc tgcttggacg
     7021 gacaggatgt atgctgtggc ttttttaagg ataactacct tgggggcctt ttcattgttt
     7081 tccaactccg ggatctggtc acgcagggca aaaaagctcc gttttagctc gttcctcctc
     7141 tggcgctcca agacgttgtg tgttcgcctc ttgacattct cctcggtgtc cgaggacctg
     7201 gggctggtgc attttcggtt gttgctgatc tgtctcagga ctctgacact gtccaacttg
     7261 accctcttgg cagcaggata gtccttccga gtggagggag gcgctgcgta gttgtgctga
     7321 tgtgtggaga cgtggcacct cttgaggacc agtgggctgt gaggaggttt gctgtggcct
     7381 ccagcagaag gtgatccaga ctctgacctt ttgccaggag cctgcctctt ttccacagaa
     7441 acaacatcga tttcttcctc atcttcttgt tcctcctcag agtcgctgct ggtggtgggc
     7501 ggtgtctcct catggagcac caggggctcg gggctgccct gcggggagga ctccgtcgag
     7561 gagagcagag aatccgagga cggagagaag gcgctggagt cttgcgaggc gcaggacttg
     7621 ggcgagctgc tgtcgttgag agggtagggg aagaccaccg aggggtcgat gcactctgag
     7681 gcggcggcgc tcagatcctg caggtacaag ctggaggtgg agcagacgct gtggccgcgg
     7741 gcggggttcg ggctgccgct gtctttgcgc gcagcctggt aggaggccag cttctctgag
     7801 acgagcttgg cggcggccga gaagccgctc cacatacagt cctggatgat gatgtttttg
     7861 atgaaggtct cgtcgtccgg gtcgcagatg aaactctggt tcaccatgtc tcctcccagc
     7921 agctcggtca ccatctccag ctggtcggcc gtggagaagc tcccgccacc gccgtcgttg
     7981 tctccccgaa gggagaaggg tgtgaccgca acgtaggagg gcgagcagag cccggagcgg
     8041 cggctagggg acaggggcgg ggtgggcagc agctcgaatt tcttccagat atcctcgctg
     8101 ggcgccgggg gctgcagctc gctctgctgc tgctgctggt agaagttctc ctcctcgtcg
     8161 cagtagaaat acggctgcac cgagtcgtag tcgaggtcat agttcctgtt ggtgaagcta
     8221 acgttgaggg gcatcgtcgc gggaggctgc tggttttcca ctacccgaaa aaaatccatg
     8281 gcgatcgcgg tacccgggcg actcagtctg tcggaggact ggcgcgccga gtgaggggtt
     8341 gtgagctctt ttatagagct cgggaagcag aagcgcgcga acagaagcga gaagcaggct
     8401 gattggttaa ttcaaataag gcgcagggtc atttcaggtc cttgggggag cctggaaaca
     8461 tctgatgggt cttaagaaac tgctgagggt tgggccatat ctggggacca tctgttcttg
     8521 gcctcgggcc ggggccgaaa ctgcggtgac catctgttct tggccccggg ccggggccga
     8581 aactgctcac cgcagatatc ctgtttggcc caacgttagc tattttcatg tacccgccct
     8641 tgatctgaac ttctctattc ttggtttggt atttttccat gccttgcaaa atggcgttac
     8701 tgcagctagc tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     8761 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     8821 cagggacagc ttcaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9301 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     9961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
    10261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10621 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    10681 cgtatcacga ggccctttcg t
//