psiCHECK-2-h1-cluster13

LOCUS      psiCHECK-2-h1-cluster13           6653 bp DNA     circular SYN 16-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2052
                     /label=h1-cluster13
     polyA_signal    2068..2116
                     /note="synthetic polyadenylation signal"
     promoter        2125..2876
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2906..2915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2912..4564
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3871..3879
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4608..4729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4862..4966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4967..5827
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5998..6586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 aattacacaa ttttaggtta accaatgatg tgattaggca aatgttttca agccaaacct
     1741 ccctcctctg ttcagaaaat gagattagca gtgcttatat gacacactcc ttgaatcatc
     1801 agtctttggt gctaatttag tcaaattagg gttatgcttc tttactaatg tagattttga
     1861 caatgtaatc caaagacatg aatctcgtaa tattaatggg aagtcacttc catttgtaga
     1921 gagagtaaag aatggacaat tgtgcccggt ccacccttat tcagtccctg atcctcaacc
     1981 ccatagccaa catgcctcac acatatccta cctataatcg aaggcagccc ttcttggttt
     2041 taccactttt tcgcggccgc tggccgcaat aaaatatctt tattttcatt acatctgtgt
     2101 gttggttttt tgtgtgagga tctaaatgag tcttcggacc tcgcgggggc cgcttaagcg
     2161 gtggttaggg tttgtctgac gcggggggag ggggaaggaa cgaaacactc tcattcggag
     2221 gcggctcggg gtttggtctt ggtggccacg ggcacgcaga agagcgccgc gatcctctta
     2281 agcacccccc cgccctccgt ggaggcgggg gtttggtcgg cgggtggtaa ctggcgggcc
     2341 gctgactcgg gcgggtcgcg cgccccagag tgtgaccttt tcggtctgct cgcagacccc
     2401 cgggcggcgc cgccgcggcg gcgacgggct cgctgggtcc taggctccat ggggaccgta
     2461 tacgtggaca ggctctggag catccgcacg actgcggtga tattaccgga gaccttctgc
     2521 gggacgagcc gggtcacgcg gctgacgcgg agcgtccgtt gggcgacaaa caccaggacg
     2581 gggcacaggt acactatctt gtcacccgga ggcgcgaggg actgcaggag cttcagggag
     2641 tggcgcagct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
     2701 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
     2761 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat
     2821 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaaaagc
     2881 ttggcattcc ggtactgttg gtaaagccac catggccgat gctaagaaca ttaagaaggg
     2941 ccctgctccc ttctaccctc tggaggatgg caccgctggc gagcagctgc acaaggccat
     3001 gaagaggtat gccctggtgc ctggcaccat tgccttcacc gatgcccaca ttgaggtgga
     3061 catcacctat gccgagtact tcgagatgtc tgtgcgcctg gccgaggcca tgaagaggta
     3121 cggcctgaac accaaccacc gcatcgtggt gtgctctgag aactctctgc agttcttcat
     3181 gccagtgctg ggcgccctgt tcatcggagt ggccgtggcc cctgctaacg acatttacaa
     3241 cgagcgcgag ctgctgaaca gcatgggcat ttctcagcct accgtggtgt tcgtgtctaa
     3301 gaagggcctg cagaagatcc tgaacgtgca gaagaagctg cctatcatcc agaagatcat
     3361 catcatggac tctaagaccg actaccaggg cttccagagc atgtacacat tcgtgacatc
     3421 tcatctgcct cctggcttca acgagtacga cttcgtgcca gagtctttcg acagggacaa
     3481 aaccattgcc ctgatcatga acagctctgg gtctaccggc ctgcctaagg gcgtggccct
     3541 gcctcatcgc accgcctgtg tgcgcttctc tcacgcccgc gaccctattt tcggcaacca
     3601 gatcatcccc gacaccgcta ttctgagcgt ggtgccattc caccacggct tcggcatgtt
     3661 caccaccctg ggctacctga tttgcggctt tcgggtggtg ctgatgtacc gcttcgagga
     3721 ggagctgttc ctgcgcagcc tgcaagacta caaaattcag tctgccctgc tggtgccaac
     3781 cctgttcagc ttcttcgcta agagcaccct gatcgacaag tacgacctgt ctaacctgca
     3841 cgagattgcc tctggcggcg ccccactgtc taaggaggtg ggcgaagccg tggccaagcg
     3901 ctttcatctg ccaggcatcc gccagggcta cggcctgacc gagacaacca gcgccattct
     3961 gattacccca gagggcgacg acaagcctgg cgccgtgggc aaggtggtgc cattcttcga
     4021 ggccaaggtg gtggacctgg acaccggcaa gaccctggga gtgaaccagc gcggcgagct
     4081 gtgtgtgcgc ggccctatga ttatgtccgg ctacgtgaat aaccctgagg ccacaaacgc
     4141 cctgatcgac aaggacggct ggctgcactc tggcgacatt gcctactggg acgaggacga
     4201 gcacttcttc atcgtggacc gcctgaagtc tctgatcaag tacaagggct accaggtggc
     4261 cccagccgag ctggagtcta tcctgctgca gcaccctaac attttcgacg ccggagtggc
     4321 cggcctgccc gacgacgatg ccggcgagct gcctgccgcc gtcgtcgtgc tggaacacgg
     4381 caagaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtga caaccgccaa
     4441 gaagctgcgc ggcggagtgg tgttcgtgga cgaggtgccc aagggcctga ccggcaagct
     4501 ggacgcccgc aagatccgcg agatcctgat caaggctaag aaaggcggca agatcgccgt
     4561 gtaataattc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga
     4621 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     4681 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     4741 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     4801 aaacctctac aaatgtggta aaatcgataa ggatccaggt ggcacttttc ggggaaatgt
     4861 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4921 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4981 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5041 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5101 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5161 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5221 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5281 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5341 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5401 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5461 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5521 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5581 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5641 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5701 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5761 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5821 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5881 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5941 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6001 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6061 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6121 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6181 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6241 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6301 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6361 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6421 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6481 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6541 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6601 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatggctc gac
//