| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD5-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001445 |
| 产品用途: | 5' AAVLINK plasmid for SETD5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETD5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD5-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD5-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD5-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD5-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD5-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD5-FLAG 6797 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6797)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6797
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3498
/codon_start=1
/label=SETD5
/translation="G*LKHHLYPKRLELSTYTKAMRIVALLVSAKTMQTC*AH*RNGSL
AI*WSRMSPSYFGLCLQSHHPLPIQAQRVPSWPHLAHLTQEKRSVEMDTASCFHQSHLL
LLLVAATLLYSLSFVTEKTWIWQK*DTLTPTLTAVLIDLPYSTQVILTWLLIPPSDPLL
RLVSQAEVEMDIRPS*ETQTRHFGQSST*CMPTPL*MLCLEQMDCIEDLL*WGIGSLYI
WMGDIVPLQKDFPADMNMA**KTSLVDPCHLVVKGPVKESHLPPRTHHRGKKYPCWSTE
NGNKKLRKILLVGEVTLHRAKASLQELGKAAVTPFPTLVPMVCRDPQPELHLPLTKNSP
HLIPLCPIWRR*AHQIPEALLHLTADLKRISAVGGWFPHQ*NDSEKEGASPRSSEAA*G
WPKRESPLPHGRVTSQRKTQTLQMEKAQRH*AQHSLKEQQFTALPDTATSSCSVIVLGQ
NHKASFSRVPPPSEDILHSLQDTVIELLH*DLETPPLTVLQNHPSLPRPIPAPPTLCPQ
TRWPHLRGHQGILAASHILETALAAIFQGGAALLVLLALPCRDPQTRQPQIQFLSPAQE
L*VPPPFLRTLGRHCHQTYGLSVCPVLGSQLSTRPPGYLRFPIHSTTHTVGVGVCTSTD
SSHCKGQESRLRRDF"
misc_feature 2703..2708
/label=SDS
misc_feature 2931..2936
/label=SDS
misc_feature 3181..3186
/label=SDS
misc_feature 3287..3292
/label=SDS
CDS 3505..3570
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3586..4017
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3730..3735
/label=SDS
repeat_region 4060..4200
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4060..4189
/label=AAV2 ITR
rep_origin 4275..4730
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5012..5116
/gene="bla"
/label=AmpR promoter
CDS 5117..5977
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6148..6736
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggat gactcaaaca
1561 tcatctgtac cccaagagac tagaactcag cacctatacc aaagcaatga gaatagtagc
1621 tcttctagta tctgcaaaga caatgcagac ttgttgagcc cattaaagaa atggaagtct
1681 cgctatctga tggagcagaa tgtcaccaag ttacttcggc ctctgtctcc agtcacacca
1741 ccccctccca attcaggctc aaagagtccc cagctggcca cacctggctc atctcaccca
1801 ggagaagagg agtgtcgaaa tggatacagc ctcatgtttt caccagtcac atctcttact
1861 actgctagtc gctgcaacac tcctctacag tttgagcttt gtcaccgaaa agacctggat
1921 ttggcaaaag taggatacct tgactccaac actaacagct gtgctgatag accttcccta
1981 ctcaactcag gtcattctga cctggctcct catccctccc tcggacccac ttctgagact
2041 ggtttcccaa gcagaagtgg agatggacat cagaccctcg tgagaaactc agaccaggca
2101 tttcggacag agttcaactt gatgtatgcc tactcccctt tgaatgctat gcctcgagca
2161 gatggactgt atcgaggatc tcctctagtg ggggatagga agcctttaca tttggatggg
2221 ggatattgtt cccctgcaga aggattttcc agcagatatg aacatggctt aatgaaagac
2281 ctctctcgtg gatccttgtc acctggtggt gaaagggcct gtgaaggagt cccatctgcc
2341 ccccagaacc caccacagag gaaaaaagta tccctgctgg agtaccgaaa acggaaacaa
2401 gaagctaagg aaaattctgc tggtggggga ggtgactctg cacagagcaa aagcaagtct
2461 gcaggagctg ggcaaggcag cagtaactcc gtttccgaca ctggtgccca tggtgtgcag
2521 ggatcctcag cccgaactcc atcttcccct cacaaaaaat tctccccatc tcattcctct
2581 atgtcccatt tggaggcggt aagcccatca gattccagag gcacttcttc atctcactgc
2641 agacctcaag agaatatcag cagtaggtgg atggttccca catcagtaga acgactccga
2701 gaaggaggga gcatccccaa ggtcctccga agcagcgtga gggtggccca aaagggagag
2761 ccctctccca catgggagag taacatcaca gagaaagact cagaccctgc agatggagaa
2821 ggcccagaga cattaagctc agcactctct aaaggagcaa cagtttacag cccttccaga
2881 tacagctacc agctcctgca gtgtgatagt cctcggacag aatcacaaag cctccttcag
2941 cagagttcct cccccttcag aggacatcct acacagtctc caggatacag ttatcgaact
3001 actgcactga gacctggaaa ccccccctct cacggttctt cagaatcatc cctctcttcc
3061 acgtcctatt ccagccccgc ccaccctgtg tccacagact cgttggcccc atttacgggg
3121 acaccagggt attttagcag ccagccacat tctggaaaca gcactggcag caatcttcca
3181 aggaggagct gcccttctag tgctgctagc cctaccctgc agggaccctc agactcgcca
3241 acctcagatt cagtttctca gtccagcaca ggaactctga gttccacctc ctttcctcag
3301 aactctaggt cgtcattgcc atcagactta cggactatca gtctgcccag tgctgggcag
3361 tcagctgtct accaggcctc cagggtatct gcggtttcca attcacagca ctacccacac
3421 cgtgggagtg ggggtgtgca ccagtaccga ctccagccac tgcaagggtc aggagtcaag
3481 actcagacgg gactttcctg tacagactac aaagaccatg acggtgatta taaagatcat
3541 gacatcgact acaaggatga cgatgacaag taagaattct gatgactcga gataatcaac
3601 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
3661 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
3721 tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg
3781 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
3841 ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt tatttgtgaa
3901 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
3961 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaaga
4021 tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
4081 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
4141 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
4201 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
4261 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
4321 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
4381 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
4441 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
4501 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
4561 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
4621 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
4681 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
4741 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
4801 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
4861 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
4921 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
4981 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
5041 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
5101 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
5161 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
5221 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
5281 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
5341 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
5401 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
5461 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
5521 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
5581 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
5641 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
5701 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
5761 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
5821 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
5881 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
5941 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
6001 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
6061 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
6121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
6181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
6241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
6301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
6361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
6421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
6481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
6541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
6601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
6661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
6721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
6781 gccttttgct cacatgt
//
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