| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001616 |
| 产品用途: | 3' AAVLINK plasmid for POLR3A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | POLR3A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG 6644 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6644)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6644
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3357
/codon_start=1
/label=POLR3A
/translation="VPSLTYHR*LPVWDSRPSVALECQTALKTGPCLILKNTQSSQLPK
ALWLIAFIPV*HQLSFSSTQWPAGKV*STRL*RQLKRDTCSEGLSNLLKIFAPSMI*QS
EALLAILSSSFMEEMA*ILQLWREKMNLWSLKGFWTTSKQSSRVPVSLLSAKTS*S*PQ
SPS*RRVSSSAARTASCRK*KNSLRGSLRRSRKPEINMASMITAQQSPVCCTSWTASPP
PK*KSFWRPVGTST*GHRWSQVLQWVLCVPRALVSQAPR*P*RLSTLQVWPP*TSPWAC
PGLKRSSTLPRPSALQLSQHS*TRMTTRIMLAS*KGELRKPSWERFPSILKKCFFLMTA
LFSSSSPWNGLGF*DWK*TLRQ*DIPSAHPSSV*SPVMWLFMVRLWCVSPPERTARAPC
TTCCSS*KRISPRWWCRAFQRCPELSSTLTSRVERRSTSFWWKVITCGQSWPHTV*RAP
EPPPITPMRWRKLWASRPPGQRSSMKSSTPW*TTA*ASTGGT*CCSPTS*PTRVKSWAS
LGLAWPR*RRVC*CWPPLRRRLTISLTLPTSGRRTLCVGCLSASSWESQ*TLEPGSSSC
FTRLTGTRTLPRGP*SSTQMNSTSPLS"
misc_feature 3037..3042
/label=SDS
misc_feature 3143..3148
/label=SDS
CDS 3364..3429
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3433..3864
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3577..3582
/label=SDS
repeat_region 3907..4047
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3907..4036
/label=AAV2 ITR
rep_origin 4122..4577
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4859..4963
/gene="bla"
/label=AmpR promoter
CDS 4964..5824
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5995..6583
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggttc cttcattaac
1561 atatcacaga tgattgcctg tgtgggacag caggccatca gtggctctcg agtgccagac
1621 ggctttgaaa acaggtcctt gcctcatttt gaaaaacact caaagctccc agctgccaaa
1681 ggctttgtgg ctaatagctt ttattccggt ttgacaccaa ctgagttttt cttccacaca
1741 atggccggcc gggaaggtct agtcgacacg gctgtaaaga cagctgaaac gggatacatg
1801 cagcgaaggc ttgtcaaatc tcttgaagat ctttgctccc agtatgatct gacagtccga
1861 agctctactg gcgatattat ccagttcatt tatggaggag atggcttaga tcctgcagct
1921 atggagggaa aagatgaacc tttggagttt aaaagggttc tggacaacat caaagcagtc
1981 ttcccgtgtc ccagtgagcc tgctctcagc aaaaacgagc tgatcctgac cacagagtcc
2041 atcatgaaga agagtgagtt cctctgctgc caggacagct tcctgcagga aataaaaaaa
2101 ttcattaagg gggtctctga gaagatcaag aaaaccagag ataaatatgg catcaatgat
2161 aacggcacaa cagagccccg tgtgctgtac cagctggacc gcatcacccc cacccaagta
2221 gaaaagtttc tggagacctg tagggacaag tacatgaggg cacagatgga gccaggttct
2281 gcagtgggtg ctctgtgtgc ccagagcatt ggtgagccag gcacccagat gaccctgaag
2341 actttccact ttgcaggtgt ggcctccatg aacatcaccc tgggcgtgcc ccggattaaa
2401 gagatcatca acgcttccaa ggccatcagc actccaatta tcacagcaca gctagacaag
2461 gatgacgacg cggattatgc tcgcctcgtg aaagggagaa ttgagaaaac cctcttggga
2521 gagatttccg agtatattga agaagtgttt cttcctgatg actgctttat tctcgtcaag
2581 ctctccctgg aacggattag gcttctgaga ctggaagtga acgctgagac agtgagatat
2641 tccatctgca catccaagct ccgtgtgaag cccggtgatg tggctgttca tggtgaggct
2701 gtggtgtgtg tcacccccag agagaacagc aagagctcca tgtactacgt gctgcagttc
2761 ctgaaagagg atctccccaa ggtggtggtg cagggcattc cagaggtgtc cagagctgtc
2821 atccacattg acgagcagag tggaaaggag aagtacaagc ttctggtgga aggtgataac
2881 ctgcgggcag tcatggccac acacggtgtg aagggcaccc gaaccacctc caataacacc
2941 tatgaggtgg agaaaactct gggcatcgag gccgcccgga caacgatcat caatgaaatc
3001 cagtacacca tggtgaacca cggcatgagc atcgacagga ggcacgtgat gctgctctcc
3061 gacctcatga cctacaaggg tgaagtcctg ggcatcacta ggtttggcct ggccaagatg
3121 aaggagagtg tgctgatgct ggcctccttt gagaagacgg ctgaccatct ctttgacgct
3181 gcctacttcg ggcagaagga ctctgtgtgt ggggtgtctg agtgcatcat catgggaatc
3241 ccaatgaaca ttggaaccgg gctcttcaag ctgcttcaca aggctgacag ggacccgaac
3301 cctcccaaga ggcccctgat cttcgacaca aatgaattcc acatccccct tgtcacatgt
3361 acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
3421 gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg
3481 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
3541 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
3601 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
3661 tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat
3721 ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
3781 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
3841 ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc
3901 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
3961 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
4021 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
4081 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
4141 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
4201 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
4261 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
4321 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
4381 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
4441 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
4501 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
4561 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
4621 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
4681 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
4741 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
4801 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
4861 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
4921 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
4981 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
5041 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
5101 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
5161 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
5221 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
5281 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
5341 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
5401 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
5461 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
5521 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
5581 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
5641 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
5701 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
5761 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
5821 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
5881 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
5941 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
6001 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
6061 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
6121 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
6181 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
6241 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
6301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
6361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
6421 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
6481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
6541 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
6601 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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