| 质粒名称: | pMOD_C2518 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 91087 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pMOD_C0000 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning) |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | TaU6 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMOD_C2518 was a gift from Daniel Voytas (Addgene plasmid # 91087 ; http://n2t.net/addgene:91087 ; RRID:Addgene_91087) |
| 参考文献部分: | A multi-purpose toolkit to enable advanced genome engineering in plants. Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. 10.1105/tpc.16.00922 PubMed 28522548 |
买家导航
pMODC2518载体基本信息
pMODC2518载体图谱质粒图谱和多克隆位点信息
pMODC2518载体简介
pMODC2518载体序列
LOCUS pMOD_C2518 3174 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA
spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol
III.
ACCESSION .
VERSION .
KEYWORDS pMOD_C2518
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3174)
AUTHORS Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E,
Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
TITLE A multi-purpose toolkit to enable advanced genome engineering in
plants.
JOURNAL Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi:
10.1105/tpc.16.00922.
PUBMED 28522548
REFERENCE 2 (bases 1 to 3174)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3174
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 80..668
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 569..588
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 822..839
/label=L4440
/note="L4440 vector, forward primer"
CDS complement(1240..1545)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind complement(1342..1361)
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_RNA 1872..1947
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 2095..2186
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
841 aagcggaaga gcgcccacct gcaacgccgg gaccaagccc gttattctga cagttctggt
901 gctcaacaca tttatattta tcaaggagca cattgttact cactgctagg agggaatcga
961 actaggaata ttgatcagag gaactacgag agagctgaag ataactgccc tctagctctc
1021 actgatctgg gtcgcatagt gagatgcagc ccacgtgagt tcagcaacgg tctagcgctg
1081 ggcttttagg cccgcatgat cgggcttttg tcgggtggtc gacgtgttca cgattgggga
1141 gagcaacgca gcagttcctc ttagtttagt cccacctcgc ctgtccagca gagttctgac
1201 cggtttataa actcgcttgc tgcatcagac ttggagacgt tatattcccc agaacatcag
1261 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
1321 aacggaaacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
1381 atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag
1441 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
1501 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
1561 tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct
1621 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc
1681 ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc
1741 aactgtcact gtaatacgct gcttcatagc atacctcctt ttgacatact tcgggtatac
1801 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg
1861 gcttcgtctc agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
1921 cttgaaaaag tggcaccgag tcggtgcttt ttttcgcgta gtcctcggta atatcgcaga
1981 acaaaagtac ctgatatcga gtgtacttca agtcacaccg gcgagtgact agcaggtgat
2041 gaaaagcctt agggtcaggt ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt
2101 tatttttcta aatacattca aatatgtatc cgctaatgag acaataaccc tgataaatgc
2161 ttcaattata ttgaaaaagg aagagtatga gtatccaaca tttccgtgtc gcccttattc
2221 ccttttttgc ggccttctgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
2281 aagatgctga agatcagttg ggtgcccgag tgggttacat tgaactggat ctcaacagcg
2341 gcaaaatctt agagagtttt cgccccgaag aacgcttccc aatgatgagc actttcaaag
2401 ttctgctatg tggcgcggtg ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc
2461 gcatacacta ttctcagaat gacttggttg agtattcacc agtcacagaa aagcatctta
2521 cggatggcat gacagtaaga gaattatgta gtgctgccat aaccatgagt gataacactg
2581 cggccaactt acttctgaca acgattggag gaccgaagga gctaaccgct tttttgcaca
2641 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
2701 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaactctc cgcaaactat
2761 taactggcga actacttact cttgcttcac gccagcaact cattgactgg atggaggcgg
2821 acaaagttgc aggaccactt ctgcgctcgg cacttccggc tggctggttt attgctgata
2881 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
2941 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
3001 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagacgaag
3061 ggaaataaat agtagcccgc ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc
3121 gcttagctac cgtaggtcct cttaggcggt ccgattagcg acatagtcat caat
//
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