| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DICER1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001607 |
| 产品用途: | 3' AAVLINK plasmid for DICER1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DICER1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DICER1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DICER1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DICER1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DICER1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DICER1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DICER1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DICER1-FLAG 6783 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6783)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6783
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3496
/codon_start=1
/label=DICER1
/translation="GRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEM
LGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDP
PVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEE
ADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPF
SSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQC
IADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNS
QQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEK
KINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPG
VLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSEL
RRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSAN
VPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARR
ALRSLKANQPQVPNS"
misc_feature 1874..1879
/label=SDS
misc_feature 1983..1988
/label=SDS
misc_feature 1986..1991
/label=SDS
misc_feature 1995..2000
/label=SDS
misc_feature 1998..2003
/label=SDS
misc_feature 2190..2195
/label=SDS
misc_feature 2799..2804
/label=SDS
misc_feature 3108..3113
/label=SDS
CDS 3503..3568
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3572..4003
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3716..3721
/label=SDS
repeat_region 4046..4186
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4046..4175
/label=AAV2 ITR
rep_origin 4261..4716
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4998..5102
/gene="bla"
/label=AmpR promoter
CDS 5103..5963
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6134..6722
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca ggatggattc
1561 tgagcagagc ccttctattg ggtactcctc aaggactctt ggccccaatc ctggacttat
1621 tcttcaggct ttgactctgt caaacgctag tgatggattt aacctggagc ggcttgaaat
1681 gcttggcgac tcctttttaa agcatgccat caccacatat ctattttgca cttaccctga
1741 tgcgcatgag ggccgccttt catatatgag aagcaaaaag gtcagcaact gtaatctgta
1801 tcgccttgga aaaaagaagg gactacccag ccgcatggtg gtgtcaatat ttgatccccc
1861 tgtgaattgg cttcctcctg gttatgtagt aaatcaagac aaaagcaaca cagataaatg
1921 ggaaaaagat gaaatgacaa aagactgcat gctggcgaat ggcaaactgg atgaggatta
1981 cgaggaggag gatgaggagg aggagagcct gatgtggagg gctccgaagg aagaggctga
2041 ctatgaagat gatttcctgg agtatgatca ggaacatatc agatttatag ataatatgtt
2101 aatggggtca ggagcttttg taaagaaaat ctctctttct cctttttcaa ccactgattc
2161 tgcatatgaa tggaaaatgc ccaaaaaatc ctccttaggt agtatgccat tttcatcaga
2221 ttttgaggat tttgactaca gctcttggga tgcaatgtgc tatctggatc ctagcaaagc
2281 tgttgaagaa gatgactttg tggtggggtt ctggaatcca tcagaagaaa actgtggtgt
2341 tgacacggga aagcagtcca tttcttacga cttgcacact gagcagtgta ttgctgacaa
2401 aagcatagcg gactgtgtgg aagccctgct gggctgctat ttaaccagct gtggggagag
2461 ggctgctcag cttttcctct gttcactggg gctgaaggtg ctcccggtaa ttaaaaggac
2521 tgatcgggaa aaggccctgt gccctactcg ggagaatttc aacagccaac aaaagaacct
2581 ttcagtgagc tgtgctgctg cttctgtggc cagttcacgc tcttctgtat tgaaagactc
2641 ggaatatggt tgtttgaaga ttccaccaag atgtatgttt gatcatccag atgcagataa
2701 aacactgaat caccttatat cggggtttga aaattttgaa aagaaaatca actacagatt
2761 caagaataag gcttaccttc tccaggcttt tacacatgcc tcctaccact acaatactat
2821 cactgattgt taccagcgct tagaattcct gggagatgcg attttggact acctcataac
2881 caagcacctt tatgaagacc cgcggcagca ctccccgggg gtcctgacag acctgcggtc
2941 tgccctggtc aacaacacca tctttgcatc gctggctgta aagtacgact accacaagta
3001 cttcaaagct gtctctcctg agctcttcca tgtcattgat gactttgtgc agtttcagct
3061 tgagaagaat gaaatgcaag gaatggattc tgagcttagg agatctgagg aggatgaaga
3121 gaaagaagag gatattgaag ttccaaaggc catgggggat atttttgagt cgcttgctgg
3181 tgccatttac atggatagtg ggatgtcact ggagacagtc tggcaggtgt actatcccat
3241 gatgcggcca ctaatagaaa agttttctgc aaatgtaccc cgttcccctg tgcgagaatt
3301 gcttgaaatg gaaccagaaa ctgccaaatt tagcccggct gagagaactt acgacgggaa
3361 ggtcagagtc actgtggaag tagtaggaaa ggggaaattt aaaggtgttg gtcgaagtta
3421 caggattgcc aaatctgcag cagcaagaag agccctccga agcctcaaag ctaatcaacc
3481 tcaggttccc aatagctgta cagactacaa agaccatgac ggtgattata aagatcatga
3541 catcgactac aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa
3601 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
3661 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
3721 ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc
3781 tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt
3841 tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat
3901 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
3961 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg
4021 tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
4081 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
4141 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
4201 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
4261 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4321 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4381 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4441 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
4501 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
4561 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
4621 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
4681 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
4741 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
4801 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
4861 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
4921 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
4981 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
5041 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
5101 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
5161 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
5221 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
5281 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
5341 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
5401 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
5461 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
5521 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
5581 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
5641 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
5701 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
5761 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
5821 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
5881 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
5941 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
6001 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
6061 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
6121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
6181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
6241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
6301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
6361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
6421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
6481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
6541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
6601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
6661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
6721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
6781 tgt
//
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