| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001649 |
| 产品用途: | 3' AAVLINK plasmid for COL1A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL1A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG 6971 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6971)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6971
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3684
/codon_start=1
/label=COL1A1
/translation="VLMALLAKMASVV*PAPLVLLALLVPLVTRVKVVPAALLVPLELV
VPPETVVSLVPPALLALLAPLVLTANLVLKANLVMLVLKAMLVPLALPDPLDPLAPLVM
LVLLEPKVLAAALVPLVLLVSLVLLAESVLLAPLEMLDPLALLVLLAKKAAKVPVVRLA
LLDVLVKLVPLVPLALLARKDPLVLMVLLVLLVLPGLKVLLDSVVWSACLVREEREASL
VFLAPLVNLANKVPLEQVVNVVPLVPWAPLDWLDPLVNLDVRGLLVPKVPLDETVLLAP
RVTVVRPAPLDPLVLLVLLVPLAPLALLARVVIVVRLVLLVPPVLSALLAPVAPPDPKA
PVVTRVRQANRATEA*RVTVASLASRVPLALLALLVNKVPLEPLVLLVPEVPLALLVLL
AKMDSTVSLAPLGPLVLAVALVMLVLLVPPALLDLLVPLVLPALVSTSASCPSHLKRRL
TMVAATTGLMMPMWFVTVTSRWTPPSRA*ASRSRTSGAQRAAARTPPAPAVTSRCATLT
GRVESTGLTPTKAATWMPSKSSATWRLVRPACTPLSPVWPRRTGTSARTPRTRGMSGSA
RA*PMDSSSSMAARAPTLPMWPSS*PSCA*CPPRPPRTSPTTARTAWPTWTSRLATSRR
PCSSRAPTRSRSAPRATAASPTASLSMAARVTPEPGARQ*LNTKPPRPPACPSSMWPPW
TLVPQTRNSASTLALSAS"
misc_feature 1603..1608
/label=SDS
misc_feature 1945..1950
/label=SDS
misc_feature 1981..1986
/label=SDS
misc_feature 2656..2661
/label=SDS
misc_feature 2836..2841
/label=SDS
misc_feature 2845..2850
/label=SDS
CDS 3691..3756
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3760..4191
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3904..3909
/label=SDS
repeat_region 4234..4374
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4234..4363
/label=AAV2 ITR
rep_origin 4449..4904
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5186..5290
/gene="bla"
/label=AmpR promoter
CDS 5291..6151
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6322..6910
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgc tgatggctct
1561 cctggcaaag atggcgtccg tggtctgacc ggccccattg gtcctcctgg ccctgctggt
1621 gcccctggtg acaagggtga aagtggtccc agcggccctg ctggtcccac tggagctcgt
1681 ggtgcccccg gagaccgtgg tgagcctggt ccccccggcc ctgctggctt tgctggcccc
1741 cctggtgctg acggccaacc tggtgctaaa ggcgaacctg gtgatgctgg tgctaaaggc
1801 gatgctggtc cccctggccc tgccggaccc gctggacccc ctggccccat tggtaatgtt
1861 ggtgctcctg gagccaaagg tgctcgcggc agcgctggtc cccctggtgc tactggtttc
1921 cctggtgctg ctggccgagt cggtcctcct ggcccctctg gaaatgctgg accccctggc
1981 cctcctggtc ctgctggcaa agaaggcggc aaaggtcccc gtggtgagac tggccctgct
2041 ggacgtcctg gtgaagttgg tccccctggt ccccctggcc ctgctggcga gaaaggatcc
2101 cctggtgctg atggtcctgc tggtgctcct ggtactcccg ggcctcaagg tattgctgga
2161 cagcgtggtg tggtcggcct gcctggtcag agaggagaga gaggcttccc tggtcttcct
2221 ggcccctctg gtgaacctgg caaacaaggt ccctctggag caagtggtga acgtggtccc
2281 cctggtccca tgggcccccc tggattggct ggaccccctg gtgaatctgg acgtgagggg
2341 gctcctggtg ccgaaggttc ccctggacga gacggttctc ctggcgccaa gggtgaccgt
2401 ggtgagaccg gccccgctgg accccctggt gctcctggtg ctcctggtgc ccctggcccc
2461 gttggccctg ctggcaagag tggtgatcgt ggtgagactg gtcctgctgg tcccgccggt
2521 cctgtcggcc ctgttggcgc ccgtggcccc gccggacccc aaggcccccg tggtgacaag
2581 ggtgagacag gcgaacaggg cgacagaggc ataaagggtc accgtggctt ctctggcctc
2641 cagggtcccc ctggccctcc tggctctcct ggtgaacaag gtccctctgg agcctctggt
2701 cctgctggtc cccgaggtcc ccctggctct gctggtgctc ctggcaaaga tggactcaac
2761 ggtctccctg gccccattgg gccccctggt cctcgcggtc gcactggtga tgctggtcct
2821 gttggtcccc ccggccctcc tggacctcct ggtccccctg gtcctcccag cgctggtttc
2881 gacttcagct tcctgcccca gccacctcaa gagaaggctc acgatggtgg ccgctactac
2941 cgggctgatg atgccaatgt ggttcgtgac cgtgacctcg aggtggacac caccctcaag
3001 agcctgagcc agcagatcga gaacatccgg agcccagagg gcagccgcaa gaaccccgcc
3061 cgcacctgcc gtgacctcaa gatgtgccac tctgactgga agagtggaga gtactggatt
3121 gaccccaacc aaggctgcaa cctggatgcc atcaaagtct tctgcaacat ggagactggt
3181 gagacctgcg tgtaccccac tcagcccagt gtggcccaga agaactggta catcagcaag
3241 aaccccaagg acaagaggca tgtctggttc ggcgagagca tgaccgatgg attccagttc
3301 gagtatggcg gccagggctc cgaccctgcc gatgtggcca tccagctgac cttcctgcgc
3361 ctgatgtcca ccgaggcctc ccagaacatc acctaccact gcaagaacag cgtggcctac
3421 atggaccagc agactggcaa cctcaagaag gccctgctcc tccagggctc caacgagatc
3481 gagatccgcg ccgagggcaa cagccgcttc acctacagcg tcactgtcga tggctgcacg
3541 agtcacaccg gagcctgggg caagacagtg attgaataca aaaccaccaa gacctcccgc
3601 ctgcccatca tcgatgtggc ccccttggac gttggtgccc cagaccagga attcggcttc
3661 gacgttggcc ctgtctgctt cctgtgtaca gactacaaag accatgacgg tgattataaa
3721 gatcatgaca tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg
3781 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
3841 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
3901 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
3961 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt
4021 gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt
4081 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
4141 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga
4201 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
4261 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
4321 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
4381 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
4441 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4501 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
4561 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4621 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
4681 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4741 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
4801 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
4861 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
4921 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
4981 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
5041 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
5101 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
5161 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
5221 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
5281 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
5341 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
5401 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
5461 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
5521 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
5581 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
5641 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
5701 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
5761 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
5821 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
5881 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
5941 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
6001 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
6061 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
6121 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
6181 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
6241 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
6301 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
6361 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
6421 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
6481 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
6541 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
6601 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
6661 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
6721 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
6781 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
6841 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
6901 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
6961 tgctcacatg t
//
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