| 质粒名称: | pTD103luxI_sfGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 48885 |
| 存储实验室: | Jeff Hasty |
|
载体骨架基本信息 |
|
| 载体名称: | pZ vector |
| 载体抗性: | 卡那霉素 |
| 载体类型: | 细菌表达，合成生物学 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长温度: | 37 ℃ |
| 宿生菌： | DH5alpha |
| 生长条件： | LB应补充0.2%葡萄糖。 |
|
插入基因信息 |
|
| 插入基因名称: | LuxI, sfGFP, and LuxR |
| 启动子 | pLux |
| 标签/融合蛋白: | SSRA标签（aandenyala）（插入件上的C端子） |
|
文献引用方法 |
|
| 材料和方法部分: | pTD103luxI_sfGFP was a gift from Jeff Hasty (Addgene plasmid # 48885 ; http://n2t.net/addgene:48885 ; RRID:Addgene_48885) |
| 参考文献部分: | A sensing array of radically coupled genetic 'biopixels'. Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. 10.1038/nature10722 PubMed 22178928 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTD103luxI_sfGFP 5563 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Positive feedback plasmid from oscillator.
ACCESSION .
VERSION .
KEYWORDS pTD103luxI_sfGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5563)
AUTHORS Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J
TITLE A sensing array of radically coupled genetic 'biopixels'.
JOURNAL Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
PUBMED 22178928
REFERENCE 2 (bases 1 to 5563)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5563
/organism="synthetic DNA construct"
/mol_type="other DNA"
RBS 968..979
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 986..1699
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(1016..1044)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 1639..1659
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
terminator 1774..1860
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
RBS 2856..2867
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
terminator 3506..3592
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(3757..4345)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3837..3856)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 4433..4527
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(4558..5352)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4669..4688)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 5279..5298
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5531..5549
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ctcgagttaa tttttaaagt atgggcaatc aattgctcct gttaaaattg ctttagaaat
61 actttggcag cggtttgttg tattgagttt catttgcgca ttggttaaat ggaaagtgac
121 agtacgctca ctgcagccta atatttttga aatatcccaa gagctttttc cttcgcatgc
181 ccacgctaaa cattcttttt ctcttttggt taaatcgttg tttgatttat tatttgctat
241 atttattttt cgataattat caactagaga aggaacaatt aatggtatgt tcatacacgc
301 atgtaaaaat aaactatcta tatagttgtc tttttctgaa tgtgcaaaac taagcattcc
361 gaagccattg ttagccgtat gaatagggaa actaaaccca gtgataagac ctgatgtttt
421 cgcttcttta attacatttg gagatttttt atttacagca ttgttttcaa atatattcca
481 attaattggt gaatgattgg agttagaata atctactata ggatcatatt ttattaaatt
541 agcgtcatca taatattgcc tccatttttt agggtaatta tctagaattg aaatatcaga
601 tttaaccata gaatgaggat aaatgatcgc gagtaaataa tattcacaat gtaccatttt
661 agtcatatca gataagcatt gattaatatc attattgctt ctacaagctt taattttatt
721 aattattctg tatgtgtcgt cggcatttat gtttttcata cccatctctt tatccttacc
781 tattgtttgt cgcaagtttt gcgtgttata tatcattaaa acggtaatgg attgacattt
841 gattctaata aattggattt ttgtcacact attgtatcgc tgggaataca attacttaac
901 ataagcacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatga
961 attcattaaa gaggagaaag gtaccatgag caaaggagaa gaacttttca ctggagttgt
1021 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tccgtggaga
1081 gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca ctactggaaa
1141 actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc aatgcttttc
1201 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
1261 tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt
1321 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga
1381 agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca atgtatacat
1441 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacgttga
1501 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
1561 tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga aagatcccaa
1621 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg
1681 catggatgag ctctacaaag cagcgaacga cgaaaattac gcccttgcag cgtaaaagct
1741 taattagctg atctagacgc gtgctagagg catcaaataa aacgaaaggc tcagtcgaaa
1801 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
1861 ccgccgccct agacctagcc gtcttcacct cgagttaatt tttaaagtat gggcaatcaa
1921 ttgctcctgt taaaattgct ttagaaatac tttggcagcg gtttgttgta ttgagtttca
1981 tttgcgcatt ggttaaatgg aaagtgacag tacgctcact gcagcctaat atttttgaaa
2041 tatcccaaga gctttttcct tcgcatgccc acgctaaaca ttctttttct cttttggtta
2101 aatcgttgtt tgatttatta tttgctatat ttatttttcg ataattatca actagagaag
2161 gaacaattaa tggtatgttc atacacgcat gtaaaaataa actatctata tagttgtctt
2221 tttctgaatg tgcaaaacta agcattccga agccattgtt agccgtatga atagggaaac
2281 taaacccagt gataagacct gatgttttcg cttctttaat tacatttgga gattttttat
2341 ttacagcatt gttttcaaat atattccaat taattggtga atgattggag ttagaataat
2401 ctactatagg atcatatttt attaaattag cgtcatcata atattgcctc cattttttag
2461 ggtaattatc tagaattgaa atatcagatt taaccataga atgaggataa atgatcgcga
2521 gtaaataata ttcacaatgt accattttag tcatatcaga taagcattga ttaatatcat
2581 tattgcttct acaagcttta attttattaa ttattctgta tgtgtcgtcg gcatttatgt
2641 ttttcatacc catctcttta tccttaccta ttgtttgtcg caagttttgc gtgttatata
2701 tcattaaaac ggtaatggat tgacatttga ttctaataaa ttggattttt gtcacactat
2761 tgtatcgctg ggaatacaat tacttaacat aagcacctgt aggatcgtac aggtttacgc
2821 aagaaaatgg tttgttatag tcgaatgaat tcattaaaga ggagaaaggt accatgacta
2881 taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa
2941 gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc
3001 ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg
3061 aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg
3121 tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa
3181 gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa
3241 tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa
3301 cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta
3361 ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta
3421 ttaatgaaca gtttaaaaaa gcagtcttaa atgcagcgaa cgacgaaaat tacgcccttg
3481 cagcgtaaac gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
3541 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
3601 ctagacctag ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
3661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg actagtgctt ggattctcac
4441 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
4501 tcattactgg atctatcaac aggagtccaa gcgagctctc gaaccccaga gtcccgctca
4561 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
4621 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
4681 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
4741 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
4801 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
4861 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
4921 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
4981 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
5041 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
5101 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
5161 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
5221 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
5281 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
5341 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg
5401 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
5461 accagagggc gccccagctg gcaattccga cgtctaagaa accattatta tcatgacatt
5521 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//
