pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

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pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG载体质粒基本信息

质粒名称: pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG
出品公司: Wekwikgene
目录编号: 0001582
产品用途: 5' AAVLINK plasmid for PCDH15 expression
存储实验室: Zhonghua Lu Lab

载体骨架基本信息
载体名称: pAAV-MCS2
表达系统: 腺病毒
启动子: pCALM1
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称: PCDH15
物种来源: H.sapiens
密码子优化: 未优化

文献引用方法
参考文献部分:
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review

pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG载体序列质粒序列多克隆位点信息

pAAVLINKv1-pCALM1-Cre-3-PCDH15-FLAG载体图谱

pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG质粒载体简介

pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG质粒序列载体序列

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG                7374 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..4087
                     /codon_start=1
                     /label=PCDH15
                     /translation="VLSM*MDLWIMRPGQAMYFESKLIPWKWSLPISEFLQKAIQLKYT
                     LRFRMKIIIPQCFRKNSTSEVYLKMQECLLLYSE*RLLIKILAIIVSWPTDS*YHQLKR
                     EKKDL*WKHIQGLSKLLCSSII*GDPTSSFKLLQLTTMGRD*AAKPMYSSPWSISWICK
                     SLFPMCLLL*WKKR*KILQRSWIAMFRNKFLVPRS*WSPLELAGMEMPFP*KITPNVT*
                     LSMQLTPKPTEPSIEMSFLNFWMANYLISIKTFSRIMGKEDAFWRSGLQRQ*PALKREE
                     KV*DTQKGPCWLWPSSSSSAAFLPSWWFWSATDSLKYVKLSVQRLHEFRPHYPRLNQQC
                     RLLHQWQRPRRRRRLRQVRISMKNLETAQFFSFSTIFNKAGEITQSQKTGNINKL*CPF
                     LPILLRLTSQLM*TDHLRATN*SLQENSHFYLMRMT*VPIIHFIRKT*VKYQQIQTFHR
                     EQIL*THFHPKYKPRVSL*GAQEKRFRGCGVSQSAYPGG**GKFQIDQRS*ICSSGKAP
                     GRKLKMKTLESVQTKEVAAIHCLQLKRQI*QRKRK*GKVKH***KEQNS*NLSLQTLHF
                     AFPGLTSHSPLCQLFQELWNSNQNLMSSVLLLSVPWNFLLQGLVFYILHSLGERHLFVC
                     YLLKPKEIFLKILPIHQTSLLLPVPFPLLLLFLLLLLLLLLLLLLLLLTFLLFLFFVLL
                     PLLLLSLFLFLLLHFFHFPFQRLVPQHHLFYLHFQLLFLHHLLLFLALHLLQLHFCPQS
                     VSV*QVLNARPT*CLPRKLSPL*HSYQQRQCVKQTLRENQKASSDTLKT*QNLKNQ*LT
                     CTVK*KKTIYAQMFQNFKLCALQK*QIWKSHLNKTRGV*TILSRELKNNLTVNLLH"
     misc_feature    1988..1993
                     /label=SDS
     misc_feature    2263..2268
                     /label=SDS
     protein_bind    2774..2785
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    2966..2971
                     /label=SDS
     misc_feature    3467..3472
                     /label=SDS
     misc_feature    3470..3475
                     /label=SDS
     misc_feature    3473..3478
                     /label=SDS
     misc_feature    3485..3490
                     /label=SDS
     misc_feature    3494..3499
                     /label=SDS
     misc_feature    3497..3502
                     /label=SDS
     misc_feature    3521..3526
                     /label=SDS
     misc_feature    3524..3529
                     /label=SDS
     misc_feature    3557..3562
                     /label=SDS
     misc_feature    3569..3574
                     /label=SDS
     misc_feature    3593..3598
                     /label=SDS
     misc_feature    3596..3601
                     /label=SDS
     misc_feature    3686..3691
                     /label=SDS
     misc_feature    3710..3715
                     /label=SDS
     CDS             4094..4159
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4163..4594
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4307..4312
                     /label=SDS
     repeat_region   4637..4777
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4637..4766
                     /label=AAV2 ITR
     rep_origin      4852..5307
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5589..5693
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5694..6554
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6725..7313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgttatct atgtgaatgg
     1501 acctctggat tatgagacca ggacaagcta tgtacttcga gtccaagctg attccctgga
     1561 agtggtcctt gccaatctcc gagttccttc aaaaagcaat acagctaaag tatacattga
     1621 gattcaggat gaaaataatc atcccccagt gtttcagaaa aaattctaca tcggaggtgt
     1681 atctgaagat gcaagaatgt ttacttctgt actcagagtg aaggctactg ataaagatac
     1741 tggcaattat agtgtcatgg cctacagact cataatacca ccaattaaag agggaaaaga
     1801 aggatttgta gtggaaacat atacagggct tatcaaaact gctatgctct tccataatat
     1861 gaggagatcc tacttcaagt ttcaagttat tgcaactgac gactatggga agggactgag
     1921 cggcaaagcc gatgtactcg tctccgtggt caatcagctg gatatgcaag tcattgtttc
     1981 caatgtgcct cctactctag tggaaaaaaa gatagaagat cttacagaga tcttggatcg
     2041 ctatgttcag gaacaaattc ctggtgccaa ggtcgtagtg gagtccattg gagctcgccg
     2101 gcatggagat gccttttccc tagaagatta caccaaatgt gacttgactg tctatgcaat
     2161 tgacccccaa accaacagag ccatcgatag aaatgagctt tttaaatttt tggatggcaa
     2221 actacttgat atcaataaag actttcagcc gtattatggg gaaggaggac gcattctgga
     2281 gatccggact ccagaggcag tgaccagcat taaaaagaga ggagaaagtc taggatacac
     2341 agaaggggcc ttgttggctc tggccttcat catcatcctc tgctgcattc ctgccatctt
     2401 ggtggttttg gtcagctaca gacagtttaa agtacgtcaa gctgagtgta caaagactgc
     2461 acgaattcag gccgcattac ccgcggctaa accagcagtg ccggctcctg caccagtggc
     2521 agcgcccccg ccgccgccgc cgcctccgcc aggtgcgcat ctctatgaag aacttggaga
     2581 cagctcaatt cttttccttc tctaccattt tcaacaaagc aggggaaata actcagtctc
     2641 agaagacagg aaacatcaac aagttgtgat gcccttttct tccaatacta ttgaggctca
     2701 caagtcagct catgtagacg gatcacttaa gagcaacaaa ctgaagtctg caagaaaatt
     2761 cacatttcta tctgatgagg atgacttaag tgcccataat ccactttata aggaaaacat
     2821 aagtcaagta tcaacaaatt cagacatttc acagagaaca gattttgtag acccattttc
     2881 acccaaaata caagccaaga gtaagtctct gaggggccca agagaaaaga ttcagaggct
     2941 gtggagtcag tcagtcagct tacccaggag gctgatgagg aaagttccaa atagaccaga
     3001 gatcatagat ctgcagcagt ggcaaggcac caggcagaaa gctgaaaatg aaaacactgg
     3061 aatctgtaca aacaaaagag gtagcagcaa tccattgctt acaactgaag aggcaaattt
     3121 gacagagaaa gaggaaataa ggcaaggtga aacactgatg atagaaggaa cagaacagtt
     3181 gaaatctctc tcttcagact cttcattttg ctttcccagg cctcacttct cattctccac
     3241 tttgccaact gtttcaagaa ctgtggaact caaatcagaa cctaatgtca tcagttctcc
     3301 tgctgagtgt tccttggaac tttctccttc aaggccttgt gttttacatt cttcactctc
     3361 taggagagag acacctattt gtatgttacc tattgaaacc gaaagaaata tttttgaaaa
     3421 ttttgcccat ccaccaaaca tctctccttc tgcctgtccc cttccccctc ctcctcctat
     3481 ttctcctcct tctcctcctc ctgctcctgc tcctcttgct cctcctcctg acatttctcc
     3541 tttttctctt ttttgtcctc ctccctctcc tccttctatc cctcttcctc ttcctcctcc
     3601 tacatttttt ccactttccg tttcaacgtc tggtccccca acaccacctc ttctacctcc
     3661 atttccaact cctcttcctc caccacctcc ttctattcct tgccctccac ctccttcagc
     3721 ttcatttctg tccacagagt gtgtctgtat aacaggtgtt aaatgcacga ccaacttgat
     3781 gcctgccgag aaaattaagt cctctatgac acagctatca acaacgacag tgtgtaaaac
     3841 agaccctcag agagaaccaa aaggcatcct cagacacgtt aaaaacttag cagaacttga
     3901 aaaatcagta gctaacatgt acagtcaaat agaaaaaaac tatctacgca caaatgtttc
     3961 agaacttcaa actatgtgcc cttcagaagt aacaaatatg gaaatcacat ctgaacaaaa
     4021 caaggggagt ttgaacaata ttgtcgaggg aactgaaaaa caatctcaca gtcaatctac
     4081 ttcactgtgt acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     4141 caaggatgac gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa
     4201 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4261 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4321 tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4381 ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct
     4441 attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4501 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4561 tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg
     4621 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4681 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     4741 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     4801 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     4861 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4921 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4981 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5041 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     5101 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5161 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     5221 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5281 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     5341 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     5401 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     5461 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     5521 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5581 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5641 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5701 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5761 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5821 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5881 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5941 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6001 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6061 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6121 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6181 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6241 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6301 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6361 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6421 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6481 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6541 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6601 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6661 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6721 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6781 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6841 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6901 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6961 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7021 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7081 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7141 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7201 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7261 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7321 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//

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pAAVLINKv1-pCALM1-Cre-3'-PCDH15-FLAG质粒载体应用举例

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