| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-NUP155-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001402 |
| 产品用途: | 5' AAVLINK plasmid for NUP155 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
NUP155
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-NUP155-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-NUP155-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-NUP155-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-NUP155-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-NUP155-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-NUP155-FLAG 6796 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6796)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6796
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3509
/codon_start=1
/label=NUP155
/translation="GLQEFLDRNSQFAGGPLGNPNTTAKVQQRLIGFMRPENGNPQQMQ
QELQRKFHEAQLSEKISLQAIQQLVRKSYQALALWKLLCEHQFTIIVAELQKELQEQLK
ITTFKDLVIRDKELTGALIASLINCYIRDNAAVDGISLHLQDICPLLYSTDDAICSKAN
ELLQRSRQVQNKTEKERMLRESLKEYQKISNQVDLSNVCAQYRQVRFYEGVVELSLTAA
EKKDPQGLGLHFYKHGEPEEDIVGLQAFQERLNSYKCITDTLQELVNQSKAAPQSPSVP
KKPGPPVLSSDPNMLSNEEAGHHFEQMLKLSQRSKDELFSIALYNWLIQVDLADKLLQV
ASPFLEPHLVRMAKVDQNRVRYMDLLWRYYEKNRSFSNAARVLSRLADMHSTEISLQQR
LEYIARAILSAKSSTAISSIAADGEFLHELEEKMEVARIQLQIQETLQRQYSHHSSVQD
AVSQLDSELMDITKLYGEFADPFKLAECKLAIIHCAGYSDPILVQTLWQDIIEKELSDS
VTLSSSDRMHALSLKIVLLGKIYAGTPRFFPLDFIVQFLEQQVCTLNWDVGFVIQTMNE
IGVPLPRLLEVYDQLFKSRDPFWNRMKKPLHLLDCIHVLLIRYVENPSQVLNCERRRFT
NLCLDAVCGYLVELQSMSSSVAVQAITGNFKSLQAKLERLH"
misc_feature 1520..1525
/label=SDS
CDS 3516..3581
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3585..4016
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3729..3734
/label=SDS
repeat_region 4059..4199
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4059..4188
/label=AAV2 ITR
rep_origin 4274..4729
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5011..5115
/gene="bla"
/label=AmpR promoter
CDS 5116..5976
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6147..6735
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggtttgcag gaatttctag
1501 acagaaactc ccagtttgca ggaggaccat taggaaatcc aaatactact gctaaagtgc
1561 agcagaggct gataggattc atgcgtcctg aaaacggaaa tccccagcaa atgcaacagg
1621 aactgcagag gaagtttcat gaggctcaac taagtgaaaa gatttcactt caggcaattc
1681 agcagttggt tcgaaaatca tatcaggctc tggctttatg gaaacttctt tgtgaacatc
1741 aattcactat cattgtggca gaacttcaga aggaacttca agagcagctg aagatcacca
1801 cctttaaaga tcttgtaatc agggacaaag aactcacagg ggcattaatt gcttctctta
1861 tcaactgcta catcagagat aatgccgctg ttgatggcat tagtttacat ttacaggata
1921 tctgcccact tctatatagc actgatgatg caatttgttc taaggcaaat gagcttctcc
1981 agcgttcccg acaagttcaa aataagactg aaaaagaaag aatgttaagg gaatcattaa
2041 aggaatatca aaaaattagc aatcaagtgg acctttccaa tgtttgtgct cagtatagac
2101 aagtgagatt ttatgagggt gtggtggaac tttctcttac ggctgcagag aaaaaagatc
2161 ctcaaggtct tgggcttcat ttctataaac atggagaacc agaagaagac atagttggac
2221 ttcaggcctt ccaagaaaga ttaaacagtt acaaatgcat tacagacaca cttcaagaac
2281 tggtaaatca aagtaaggcc gctcctcagt ctcccagtgt acccaaaaaa cctggtcctc
2341 cagtgttgtc atctgatcca aatatgctga gtaatgaaga agcaggacat cattttgaac
2401 aaatgcttaa attgtcacag cgatccaagg atgagctctt tagtattgcc ctttataatt
2461 ggctaataca agtcgacctt gcagataagc tgctacaggt tgcttctcca tttctggagc
2521 cacatctagt ccgaatggcc aaagttgatc aaaacagagt tcgttatatg gatttactct
2581 ggcggtatta cgagaagaac agaagtttca gtaatgctgc tcgtgtactg tccagactgg
2641 ctgacatgca tagcacagaa atttcacttc agcagcgact agagtacatt gctcgagcca
2701 ttcttagtgc caaaagttcc actgccattt catcaatagc tgccgatggt gaatttcttc
2761 atgaattaga agaaaaaatg gaggttgcta ggatccaact tcagatacag gagacactac
2821 aaaggcagta ttcccatcat tcttctgtac aggatgcagt ttctcagctg gattctgagc
2881 tgatggacat aactaagctt tatggggaat ttgctgaccc atttaaactt gcagagtgca
2941 aacttgcaat aattcattgt gccggttatt cagaccctat attggtgcag acactttggc
3001 aagatatcat agagaaagaa ttgagtgaca gtgtgacatt gagctcctcg gatagaatgc
3061 atgctcttag tctcaagatt gttctccttg gcaaaattta tgctggcaca ccacgcttct
3121 ttcctttaga ttttattgta cagtttttag aacagcaggt ttgtactttg aactgggatg
3181 tgggcttcgt aatacagaca atgaatgaaa ttggagtacc attacctaga ctactagaag
3241 tttatgatca gttgttcaaa tcacgggatc cattctggaa cagaatgaag aagccactgc
3301 accttttgga ttgtatacat gtattattga taagatatgt tgagaatccc agccaagttt
3361 taaattgtga aaggagaaga tttacaaatc tctgcctgga tgctgtttgt ggttaccttg
3421 ttgagctcca gtctatgagc tcgtcagtag cagtacaagc catcactggg aattttaaat
3481 ctcttcaagc taaattagaa cggcttcatt gtacagacta caaagaccat gacggtgatt
3541 ataaagatca tgacatcgac tacaaggatg acgatgacaa gtaactcgag ataatcaacc
3601 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
3661 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
3721 cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc
3781 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
3841 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa
3901 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
3961 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat
4021 ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
4081 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
4141 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
4201 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
4261 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
4321 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
4381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
4441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
4501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
4561 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
4621 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
4681 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
4741 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4801 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
4861 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
4921 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
4981 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
5041 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
5101 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
5161 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
5221 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
5281 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
5341 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
5401 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
5461 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
5521 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
5581 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5641 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5701 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5761 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5821 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
5881 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
5941 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
6001 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
6061 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
6121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
6181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
6241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
6301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
6421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
6481 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
6541 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
6601 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6661 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6721 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6781 ccttttgctc acatgt
//
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