| 质粒名称: | pHR_SFFV_LaG17_synNotch_TetRVP64 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79128 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LaG17 GFP nanobody synNotch TetRVP64 |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_SFFV_LaG17_synNotch_TetRVP64 was a gift from Wendell Lim (Addgene plasmid # 79128 ; http://n2t.net/addgene:79128 ; RRID:Addgene_79128) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_SFFV_LaG17_synNotch_TetRVP64 11269 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector for constitutive expression of the LaG17 GFP
nanobody synNotch TetRVP64 Receptor (SFFV promoter).
ACCESSION .
VERSION .
KEYWORDS pHR_SFFV_LaG17_synNotch_TetRVP64
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11269)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 11269)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11269
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 85..318
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(343..361)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(344..361)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(651..670)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(884..903)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1003..1025
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1063..1081)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1149..1253
/gene="bla"
/label=AmpR promoter
CDS 1254..2114
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1472..1491)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2285..2873
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2774..2793
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3027..3044
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(3114..3134)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3119..3448
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3299..3434
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3361..3380
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 4582..4647
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4777..4797
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5222..5356
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5259..5278)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5313..5332
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 5524..6157
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 6204..6329
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6826..7059
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7244..7288
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 7573..7690
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 7843..8250
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
regulatory 8318..8327
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 8387..8416
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
protein_bind 9779..9799
/gene="mutant version of attB"
/label=attB5
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
CDS 9800..10546
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=tTA-Advanced
/note="In the Tet-Off(R) system, tTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the absence of tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
misc_feature 10656..11244
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(10709..10729)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(11127..11138)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(11247..11263)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11248..11264)
/label=pBluescriptKS
/note="For pBluescript vector"
ORIGIN
1 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
61 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
121 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
181 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
241 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
301 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
361 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
421 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
481 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
541 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
601 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
661 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
721 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
781 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
841 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
901 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
961 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
1021 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
1081 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
1141 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
1201 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
1261 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
1321 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
1381 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
1441 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
1501 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
1561 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
1621 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
1681 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
1741 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
1801 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
1861 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
1921 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
1981 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
2041 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
2101 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
2161 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
2221 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
2281 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
2341 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
2401 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
2461 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
2521 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
2581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
2641 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
2701 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
2761 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
2821 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
2881 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
2941 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
3001 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
3061 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
3121 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
3181 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
3241 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
3301 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
3361 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
3421 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
3481 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
3541 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
3601 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
3661 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
3721 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
3781 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
3841 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
3901 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
3961 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
4021 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
4081 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
4141 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
4201 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
4261 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
4321 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
4381 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
4441 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
4501 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
4561 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
4621 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
4681 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
4741 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
4801 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
4861 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
4921 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
4981 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
5041 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
5101 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
5161 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
5221 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
5281 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
5341 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
5401 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
5461 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
5521 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
5581 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
5641 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
5701 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
5761 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
5821 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
5881 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
5941 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
6001 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
6061 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
6121 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
6181 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
6241 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
6301 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
6361 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
6421 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
6481 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
6541 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
6601 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
6661 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
6721 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
6781 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
6841 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
6901 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
6961 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
7021 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
7081 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
7141 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
7201 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
7261 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
7321 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
7381 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
7441 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
7501 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt
7561 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
7621 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
7681 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga
7741 tatcgaattc ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg
7801 ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag
7861 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
7921 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
7981 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
8041 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
8101 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
8161 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
8221 ccagtcctcc gacagactga gtcgcccggg ggggatctgg agctctcgag aattctcacg
8281 cgtcaagtgg agcaaggcag gtggacagtg gatcgccgcc accatggcat tgcccgtgac
8341 cgccctgctg ctgccactgg ccttgttgct ccacgccgcg cggccagaac agaagctgat
8401 cagcgaggag gatctgatgg ctgatgtgca actcgtagag tccggtggcg gactcgtcca
8461 ggcgggaggc tccctgcgcc tgagttgcgc tgcgtccgga agaactatta gcatggccgc
8521 catgagttgg ttcagacagg cgcctggaaa ggagagggag ttcgtggcag gcataagtcg
8581 ctccgcaggg tcagccgtcc atgccgactc cgtaaaggga cgctttacca tctccagaga
8641 taacacaaaa aacacgctct atctccaaat gaattctttg aaggccgagg acacagccgt
8701 gtactactgc gccgttagga ccagcggatt ttttggaagc attcctagga cagggacagc
8761 ctttgattac tggggccagg gaacgcaggt taccgtttcc atcctggact acagcttcac
8821 aggtggcgct gggcgcgaca ttcccccacc gcagattgag gaggcctgtg agctgcctga
8881 gtgccaggtg gatgcaggca ataaggtctg caacctgcag tgtaataatc acgcatgtgg
8941 ctgggatggt ggcgactgct ccctcaactt caatgacccc tggaagaact gcacgcagtc
9001 tctacagtgc tggaagtatt ttagcgacgg ccactgtgac agccagtgca actcggccgg
9061 ctgcctcttt gatggcttcg actgccagct caccgaggga cagtgcaacc ccctgtatga
9121 ccagtactgc aaggaccact tcagtgatgg ccactgcgac cagggctgta acagtgccga
9181 atgtgagtgg gatggcctag actgtgctga gcatgtaccc gagcggctgg cagccggcac
9241 cctggtgctg gtggtgctgc ttccacccga ccagctacgg aacaactcct tccactttct
9301 gcgggagctc agccacgtgc tgcacaccaa cgtggtcttc aagcgtgatg cgcaaggcca
9361 gcagatgatc ttcccgtact atggccacga ggaagagctg cgcaagcacc caatcaagcg
9421 ctctacagtg ggttgggcca cctcttcact gcttcctggt accagtggtg ggcgccagcg
9481 cagggagctg gaccccatgg acatccgtgg ctccattgtc tacctggaga tcgacaaccg
9541 gcaatgtgtg cagtcatcct cgcagtgctt ccagagtgcc accgatgtgg ctgccttcct
9601 aggtgctctt gcgtcacttg gcagcctcaa tattccttac aagattgagg ccgtgaagag
9661 tgagccggtg gagcctccgc tgccctcgca gctgcacctc atgtacgtgg cagcggccgc
9721 cttcgtgctc ctgttctttg tgggctgtgg ggtgctgctg tcccgcaagc gccggcggca
9781 actttgtata caaaagttga tgtctagact ggacaagagc aaagtcataa actctgctct
9841 ggaattactc aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
9901 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
9961 cctggcaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
10021 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
10081 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
10141 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
10201 gtccgccgtg ggccacttta cactgggctg cgtattggag gatcaggagc atcaagtagc
10261 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
10321 tgagctgttc gaccatcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
10381 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg cccttgacga
10441 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
10501 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaagatc cttgacttgc
10561 ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc
10621 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
10681 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
10741 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
10801 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
10861 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
10921 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
10981 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
11041 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
11101 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
11161 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
11221 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaa
//
