| 质粒名称: | pAAVLINK-EFS-HA-5'-DICER1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001605 |
| 产品用途: | 5' AAVLINK plasmid for DICER1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DICER1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-5'-DICER1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-5'-DICER1载体质粒基本信息
pAAVLINK-EFS-HA-5'-DICER1质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-DICER1载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-DICER1质粒载体简介
pAAVLINK-EFS-HA-5'-DICER1质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-DICER1 7080 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7080)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7080
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 372..398
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 405..4220
/codon_start=1
/label=DICER1
/translation="MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPR
KYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSAN
QVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGY
LSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEK
IQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFI
NDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEEL
HRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVE
WYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLN
RLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLI
ATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDL
KTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHIN
RYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVV
ALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAI
PECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQ
IPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTD
ADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDA
VIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLD
VDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLW
RKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSF
ISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHV
EVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSY
ENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLK"
misc_feature 2784..2789
/label=SDS
misc_feature 3823..3828
/label=SDS
intron 4221..4261
/label=Splicing Donor
misc_feature 4265..4298
/label=lox2272-JT1571
repeat_region 4343..4483
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4343..4472
/label=AAV2 ITR
rep_origin 4558..5013
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5295..5399
/gene="bla"
/label=AmpR promoter
CDS 5400..6260
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6431..7019
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cggccaccat gtacccttat gacgtacctg actatgctgg atctatgaaa agccctgctt
421 tgcaacccct cagcatggca ggcctgcagc tcatgacccc tgcttcctca ccaatgggtc
481 ctttctttgg actgccatgg caacaagaag caattcatga taacatttat acgccaagaa
541 aatatcaggt tgaactgctt gaagcagctc tggatcataa taccatcgtc tgtttaaaca
601 ctggctcagg gaagacattt attgcagtac tactcactaa agagctgtcc tatcagatca
661 ggggagactt cagcagaaat ggaaaaagga cggtgttctt ggtcaactct gcaaaccagg
721 ttgctcaaca agtgtcagct gtcagaactc attcagatct caaggttggg gaatactcaa
781 acctagaagt aaatgcatct tggacaaaag agagatggaa ccaagagttt actaagcacc
841 aggttctcat tatgacttgc tatgtcgcct tgaatgtttt gaaaaatggt tacttatcac
901 tgtcagacat taaccttttg gtgtttgatg agtgtcatct tgcaatccta gaccacccct
961 atcgagaaat tatgaagctc tgtgaaaatt gtccatcatg tcctcgcatt ttgggactaa
1021 ctgcttccat tttaaatggg aaatgtgatc cagaggaatt ggaagaaaag attcagaaac
1081 tagagaaaat tcttaagagt aatgctgaaa ctgcaactga cctggtggtc ttagacaggt
1141 atacttctca gccatgtgag attgtggtgg attgtggacc atttactgac agaagtgggc
1201 tttatgaaag actgctgatg gaattagaag aagcacttaa ttttatcaat gattgtaata
1261 tatctgtaca ttcaaaagaa agagattcta ctttaatttc gaaacagata ctatcagact
1321 gtcgtgccgt attggtagtt ctgggaccct ggtgtgcaga taaagtagct ggaatgatgg
1381 taagagaact acagaaatac atcaaacatg agcaagagga gctgcacagg aaatttttat
1441 tgtttacaga cactttccta aggaaaatac atgcactatg tgaagagcac ttctcacctg
1501 cctcacttga cctgaaattt gtaactccta aagtaatcaa actgctcgaa atcttacgca
1561 aatataaacc atatgagcga cagcagtttg aaagcgttga gtggtataat aatagaaatc
1621 aggataatta tgtgtcatgg agtgattctg aggatgatga tgaggatgaa gaaattgaag
1681 aaaaagagaa gccagagaca aattttcctt ctccttttac caacattttg tgcggaatta
1741 tttttgtgga aagaagatac acagcagttg tcttaaacag attgataaag gaagctggca
1801 aacaagatcc agagctggct tatatcagta gcaatttcat aactggacat ggcattggga
1861 agaatcagcc tcgcaacaaa cagatggaag cagaattcag aaaacaggaa gaggtactta
1921 ggaaatttcg agcacatgag accaacctgc ttattgcaac aagtattgta gaagagggtg
1981 ttgatatacc aaaatgcaac ttggtggttc gttttgattt gcccacagaa tatcgatcct
2041 atgttcaatc taaaggaaga gcaagggcac ccatctctaa ttatataatg ttagcggata
2101 cagacaaaat aaaaagtttt gaagaagacc ttaaaaccta caaagctatt gaaaagatct
2161 tgagaaacaa gtgttccaag tcggttgata ctggtgagac tgacattgat cctgtcatgg
2221 atgatgatga cgttttccca ccatatgtgt tgaggcctga cgatggtggt ccacgagtca
2281 caatcaacac ggccattgga cacatcaata gatactgtgc tagattacca agtgatccgt
2341 ttactcatct agctcctaaa tgcagaaccc gagagttgcc tgatggtaca ttttattcaa
2401 ctctttatct gccaattaac tcacctcttc gagcctccat tgttggtcca ccaatgagct
2461 gtgtacgatt ggctgaaaga gttgtagctc tcatttgctg tgagaaactg cacaaaattg
2521 gcgaactgga tgaccatttg atgccagttg ggaaagagac tgttaaatat gaagaggagc
2581 ttgatttgca tgatgaagaa gagaccagtg ttccaggaag accaggttcc acgaaacgaa
2641 ggcagtgcta cccaaaagca attccagagt gtttgaggga tagttatccc agacctgatc
2701 agccctgtta cctgtatgtg ataggaatgg ttttaactac acctttacct gatgaactca
2761 actttagaag gcggaagctc tatcctcctg aagataccac aagatgcttt ggaatactga
2821 cggccaaacc catacctcag attccacact ttcctgtgta cacacgctct ggagaggtta
2881 ccatatccat tgagttgaag aagtctggtt tcatgttgtc tctacaaatg cttgagttga
2941 ttacaagact tcaccagtat atattctcac atattcttcg gcttgaaaaa cctgcactag
3001 aatttaaacc tacagacgct gattcagcat actgtgttct acctcttaat gttgttaatg
3061 actccagcac tttggatatt gactttaaat tcatggaaga tattgagaag tctgaagctc
3121 gcataggcat tcccagtaca aagtatacaa aagaaacacc ctttgttttt aaattagaag
3181 attaccaaga tgccgttatc attccaagat atcgcaattt tgatcagcct catcgatttt
3241 atgtagctga tgtgtacact gatcttaccc cactcagtaa atttccttcc cctgagtatg
3301 aaacttttgc agaatattat aaaacaaagt acaaccttga cctaaccaat ctcaaccagc
3361 cactgctgga tgtggaccac acatcttcaa gacttaatct tttgacacct cgacatttga
3421 atcagaaggg gaaagcgctt cctttaagca gtgctgagaa gaggaaagcc aaatgggaaa
3481 gtctgcagaa taaacagata ctggttccag aactctgtgc tatacatcca attccagcat
3541 cactgtggag aaaagctgtt tgtctcccca gcatacttta tcgccttcac tgccttttga
3601 ctgcagagga gctaagagcc cagactgcca gcgatgctgg cgtgggagtc agatcacttc
3661 ctgcggattt tagataccct aacttagact tcgggtggaa aaaatctatt gacagcaaat
3721 ctttcatctc aatttctaac tcctcttcag ctgaaaatga taattactgt aagcacagca
3781 caattgtccc tgaaaatgct gcacatcaag gtgctaatag aacctcctct ctagaaaatc
3841 atgaccaaat gtctgtgaac tgcagaacgt tgctcagcga gtcccctggt aagctccacg
3901 ttgaagtttc agcagatctt acagcaatta atggtctttc ttacaatcaa aatctcgcca
3961 atggcagtta tgatttagct aacagagact tttgccaagg aaatcagcta aattactaca
4021 agcaggaaat acccgtgcaa ccaactacct catattccat tcagaattta tacagttacg
4081 agaaccagcc ccagcccagc gatgaatgta ctctcctgag taataaatac cttgatggaa
4141 atgctaacaa atctacctca gatggaagtc ctgtgatggc cgtaatgcct ggtacgacag
4201 acactattca agtgctcaag gtaagtatca aggttacaag acaggtttaa ggagaccaat
4261 aagctattat tcgtatagga tactttatac gaagttatta agttatggag accggtgtgg
4321 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
4381 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
4441 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
4501 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
4561 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
4621 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
4681 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
4741 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
4801 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
4861 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
4921 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
4981 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
5041 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
5101 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
5161 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
5221 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
5281 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
5341 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
5401 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
5461 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
5521 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
5581 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
5641 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
5701 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
5761 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
5821 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
5881 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
5941 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
6001 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
6061 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
6121 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
6181 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
6241 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
6301 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
6361 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
6421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
6481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
6541 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
6601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
6661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
6721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
6781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
6841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
6901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
6961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
7021 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
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pAAVLINK-EFS-HA-5'-DICER1质粒载体应用举例
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