| 质粒名称: | pLX208 CMV sTurboID (C)-HA-FRB-ERM |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 153007 |
| 存储实验室: | Alice Ting |
|
载体骨架基本信息 |
|
| 载体名称: | pLX208 |
| 空载体大小: | 8233 bp |
| 完整质粒大小: | 9526 bp |
| 载体类型: | Mammalian Expression, Lentiviral |
| 筛选标记: | sHygromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | TurboID (C) (G74)-ERM (Cb5) |
| 插入大小: | 1290bp |
| 启动子: | CMV |
| 标签/融合蛋白: |
FRB
HA
|
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | CGC AAA TGG GCG GTA GGC GTG |
|
文献引用方法 |
|
| 材料和方法部分: | pLX208 CMV sTurboID (C)-HA-FRB-ERM was a gift from Alice Ting (Addgene plasmid # 153007 ; http://n2t.net/addgene:153007 ; RRID:Addgene_153007) |
| 参考文献部分: | Split-TurboID enables contact-dependent proximity labeling in cells. Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak C, Rhee HW, Lee IK, Carr SA, Ting AY. Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 10.1073/pnas.1919528117. 10.1073/pnas.1919528117 PubMed 32424107 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-HA-FRB-ERM153007质粒图谱.png)
载体简介
载体序列
LOCUS pLX208 CMV sTurboID (C)-HA-FRB-ERM 9523 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses split-TurboID C-terminal fragment targeted to endoplasmic
reticulum membrane.
ACCESSION .
VERSION .
KEYWORDS pLX208 CMV sTurboID (C)-HA-FRB-ERM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9523)
AUTHORS Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak
C, Rhee HW, Lee IK, Carr SA, Ting AY
TITLE Split-TurboID enables contact-dependent proximity labeling in cells.
JOURNAL Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi:
10.1073/pnas.1919528117.
PUBMED 32424107
REFERENCE 2 (bases 1 to 9523)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9523
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 5..231
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 232..412
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 459..584
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1077..1310
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1495..1539
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 1714..2214
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 2133..2151
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 2226..3245
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
"
misc_feature 3302..3419
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 3536..3839
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3840..4043
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3994..4014
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 4066..4075
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 5380..5968
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5433..5453)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5851..5862)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6040..6273
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6351..6472
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6388..6407)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6442..6461
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6512..6647
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6574..6593
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6667..6686)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6668..6686)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6696..6713)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6696..6712)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6705..6727)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6854..7309
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6941..6960)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7151..7172
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7335..7439
/gene="bla"
/label=AmpR promoter
CDS 7440..8300
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7658..7677)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8471..9059
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8960..8979
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9213..9230
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 9347..9368
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9383..9413
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9421..9437
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9426..9448
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 9445..9461
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9445..9461
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9480..9500
/label=T3
/note="T3 promoter, forward primer"
promoter 9482..9500
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
1681 tttcagaccc acctcccaac cccgagggga cccttgcgcc ttttccaagg cagccctggg
1741 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
1801 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
1861 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
1921 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
1981 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
2041 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
2101 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
2161 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta
2221 ccaccatgaa aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg atcgaaaagt
2281 tcgacagcgt ctccgacctg atgcagctct ccgagggcga agaatctcgg gctttcagct
2341 tcgatgtggg agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat ggtttctaca
2401 aagatcgcta tgtttatcgg cactttgcat ccgccgctct ccctattccc gaagtgcttg
2461 acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
2521 ccttgcaaga cctgcctgaa accgaactgc ccgctgttct ccagcccgtc gccgaggcca
2581 tggatgccat cgctgccgcc gatcttagcc agaccagcgg gttcggccca ttcggacctc
2641 aaggaatcgg tcaatacact acatggcgcg atttcatctg cgctattgct gatccccatg
2701 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc caggctctcg
2761 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgccgatt
2821 tcggctccaa caatgtcctg accgacaatg gccgcataac agccgtcatt gactggagcg
2881 aggccatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccctggt
2941 tggcttgtat ggagcagcag acccgctact tcgagcggag gcatcccgag cttgcaggat
3001 ctcctcggct ccgggcttat atgctccgca ttggtcttga ccaactctat cagagcttgg
3061 ttgacggcaa tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca atcgtccggt
3121 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc gtctggaccg
3181 atggctgtgt ggaagtgctc gccgatagtg gaaacagacg ccccagcact cgtcctaggg
3241 caaaggatct gcagtaatga gaattctaga tcttgagaca aatggcagta ttcatccaca
3301 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
3361 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
3421 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt
3481 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
3541 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
3601 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
3661 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
3721 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
3781 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
3841 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
3901 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
3961 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
4021 gggaggtcta tataagcaga gctctctggc taactgtcgg gatccgccac catgggacag
4081 ctggacggcg ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg
4141 gatcgaatcg gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg
4201 agaggaagca gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg
4261 ttttggcgcc tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc
4321 atggcagaag cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac
4381 ctgtatctgc aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc
4441 gatgctgcac agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa
4501 agcgtggtca atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat
4561 actctggccg ctacgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa
4621 ggcctggctc catatctgcc acggtgggag aagctggata acttcatcaa tagacccgtg
4681 aagctgatca ttggggacaa agagattttc gggattagcc gggggattga taaacaggga
4741 gccctgctgc tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg
4801 cggtctgccg aaaagggctc gggcgggagt accggttacc catacgacgt tccagactac
4861 ggtagtggga gcggtgtggc catcctgtgg cacgagatgt ggcatgaagg cctggaagag
4921 gcatctcgtt tgtactttgg ggaaaggaac gtgaaaggca tgtttgaggt gctggagccc
4981 ttgcatgcta tgatggaacg gggcccccag actctgaagg aaacatcctt taatcaggcc
5041 tatggtcgag atttaatgga ggcccaagag tggtgcagga agtacatgaa atcagggaat
5101 gtcaaggacc tcacccaagc ctgggacctc tattatcatg tgttccgacg aatctcaagt
5161 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtcct
5221 cgagctcaag cttcgaattc gagtgctggt ggtatcacca ccgtggagtc caactcctcc
5281 tggtggacca actgggtgat ccccgccatc tccgccctgg tggtggccct gatgtaccgc
5341 ctgtacatgg ccgaggacta gtaaacgcgt taagtcgaca atcaacctct ggattacaaa
5401 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
5461 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
5521 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
5581 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
5641 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
5701 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
5761 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
5821 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
5881 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
5941 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
6001 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
6061 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
6121 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
6181 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
6241 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
6301 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
6361 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
6421 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
6481 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
6541 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
6601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
6661 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
6721 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
6781 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
6841 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
6901 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
6961 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
7021 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
7081 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
7141 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
7201 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
7261 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
7321 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
7381 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
7441 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
7501 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
7561 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
7621 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
7681 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
7741 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
7801 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
7861 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
7921 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
7981 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
8041 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
8101 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
8161 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
8221 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
8281 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
8341 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
8401 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
8461 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
8521 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
8581 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
8641 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
8701 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
8761 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
8821 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
8881 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
8941 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
9001 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
9061 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
9121 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
9181 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
9241 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
9301 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
9361 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
9421 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
9481 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
