| 质粒名称: | pAAVLINK-EF1a-HA-5'-AGL |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001650 |
| 产品用途: | 5' AAVLINK plasmid for AGL expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | AGL |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-AGL
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-AGL载体质粒基本信息
pAAVLINK-EF1a-HA-5'-AGL质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-AGL载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-AGL质粒载体简介
pAAVLINK-EF1a-HA-5'-AGL质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-AGL 6087 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6087)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6087
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3227
/codon_start=1
/label=AGL
/translation="MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAVT
VYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGG
GYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPL
QTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTA
ANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHM
NSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPE
YRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNC
LLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAH
NGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITA
TYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSL
IREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHR
SAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNF
QSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRN
PKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQL
NESKIVKQAGVATK"
intron 3228..3268
/label=Splicing Donor
misc_feature 3272..3305
/label=lox2272-JT1571
repeat_region 3350..3490
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3350..3479
/label=AAV2 ITR
rep_origin 3565..4020
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4302..4406
/gene="bla"
/label=AmpR promoter
CDS 4407..5267
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5438..6026
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gggacacagt aaacagattc gaattttact
781 tctgaacgaa atggagaaac tggaaaagac cctcttcaga cttgaacaag ggtatgagct
841 acagttccga ttaggcccaa ctttacaggg aaaagcagtt accgtgtata caaattaccc
901 atttcctgga gaaacattta atagagaaaa attccgttct ctggattggg aaaatccaac
961 agaaagagaa gatgattctg ataaatactg taaacttaat ctgcaacaat ctggttcatt
1021 tcagtattat ttccttcaag gaaatgagaa aagtggtgga ggttacatag ttgtggaccc
1081 cattttacgt gttggtgctg ataatcatgt gctacccttg gactgtgtta ctcttcagac
1141 atttttagct aagtgtttgg gaccttttga tgaatgggaa agcagactta gggttgcaaa
1201 agaatcaggc tacaacatga ttcattttac cccattgcag actcttggac tatctaggtc
1261 atgctactcc cttgccaatc agttagaatt aaatcctgac ttttcaagac ctaatagaaa
1321 gtatacctgg aatgatgttg gacagctagt ggaaaaatta aaaaaggaat ggaatgttat
1381 ttgtattact gatgttgtct acaatcatac tgctgctaat agtaaatgga tccaggaaca
1441 tccagaatgt gcctataatc ttgtgaattc tccacactta aaacctgcct gggtcttaga
1501 cagagcactt tggcgtttct cctgtgatgt tgcagaaggg aaatacaaag aaaagggaat
1561 acctgctttg attgaaaatg atcaccatat gaattccatc cgaaaaataa tttgggagga
1621 tatttttcca aagcttaaac tctgggaatt tttccaagta gatgtcaaca aagcggttga
1681 gcaatttaga agacttctta cacaagaaaa taggcgagta accaagtctg atccaaacca
1741 acaccttacg attattcaag atcctgaata cagacggttt ggctgtactg tagatatgaa
1801 cattgcacta acgactttca taccacatga caaggggcca gcagcaattg aagaatgctg
1861 taattggttt cataaaagaa tggaggaatt aaattcagag aagcatcgac tcattaacta
1921 tcatcaggaa caggcagtta attgcctttt gggaaatgtg ttttatgaac gactggctgg
1981 ccatggtcca aaactaggac ctgtcactag aaagcatcct ttagttacca ggtattttac
2041 tttcccattt gaagagatag acttctccat ggaagaatct atgattcatc tgccaaataa
2101 agcttgtttt ctgatggcac acaatggatg ggtaatggga gatgatcctc ttcgaaactt
2161 tgctgaaccg ggttcagaag tttacctaag gagagaactt atttgctggg gagacagtgt
2221 taaattacgc tatgggaata aaccagagga ctgtccttat ctctgggcac acatgaaaaa
2281 atacactgaa ataactgcaa cttatttcca gggagtacgt cttgataact gccactcaac
2341 acctcttcac gtagctgagt acatgttgga tgctgctagg aatttgcaac ccaatttata
2401 tgtagtagct gaactgttca caggaagtga agatctggac aatgtctttg ttactagact
2461 gggcattagt tccttaataa gagaggcaat gagtgcatat aatagtcatg aagagggcag
2521 attagtttac cgatatggag gagaacctgt tggatccttt gttcagccct gtttgaggcc
2581 tttaatgcca gctattgcac atgccctgtt tatggatatt acgcatgata atgagtgtcc
2641 tattgtgcat agatcagcgt atgatgctct tccaagtact acaattgttt ctatggcatg
2701 ttgtgctagt ggaagtacaa gaggctatga tgaattagtg cctcatcaga tttcagtggt
2761 ttctgaagaa cggttttaca ctaagtggaa tcctgaagca ttgccttcaa acacaggtga
2821 agttaatttc caaagcggca ttattgcagc caggtgtgct atcagtaaac ttcatcagga
2881 gcttggagcc aagggtttta ttcaggtgta tgtggatcaa gttgatgaag acatagtggc
2941 agtaacaaga cactcaccta gcatccatca gtctgttgtg gctgtatcta gaactgcttt
3001 caggaatccc aagacttcat tttacagcaa ggaagtgcct caaatgtgca tccctggcaa
3061 aattgaagaa gtagttcttg aagctagaac tattgagaga aacacgaaac cttataggaa
3121 ggatgagaat tcaatcaatg gaacaccaga tatcacagta gaaattagag aacatattca
3181 gcttaatgaa agtaaaattg ttaaacaagc tggagttgcc acaaaaggta agtatcaagg
3241 ttacaagaca ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa
3301 gttattaagt tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
3361 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
3421 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
3481 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
3541 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
3601 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
3661 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
3721 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
3781 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
3841 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
3901 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
3961 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
4021 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
4081 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
4141 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
4201 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
4261 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
4321 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
4381 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
4441 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
4501 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
4561 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
4621 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
4681 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
4741 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
4801 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
4861 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
4921 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
4981 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
5041 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
5101 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
5161 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
5221 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
5281 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
5341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
5581 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
5641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
5701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
5761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
5821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
5881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
5941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
6001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
6061 ccttttgctg gccttttgct cacatgt
//
pAAVLINK-EF1a-HA-5'-AGL载体图谱质粒图谱pdf版和pAAVLINK-EF1a-HA-5'-AGL载体序列质粒序列等相关资料下载
pAAVLINK-EF1a-HA-5'-AGL质粒载体应用举例
- 上一篇：pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL1A1-FLAG
- 下一篇：没有了
