| 质粒名称: | pLR16-Phes |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 98783 |
| 存储实验室: | Anat Herskovits |
|
载体骨架基本信息 |
|
| 载体名称: | An pKSV7oriT derivative vector (pBHE261) and pPL2 |
| 载体出品公司: | Clontech |
| 空载体大小: | 7703bp |
| 完整质粒大小: | 7703bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Chloramphenicol, 25 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | pheS |
| 物种来源: | Bacillus subtillis |
| 插入大小: | 951 bp |
| 基因突变: | A309G |
|
文献引用方法 |
|
| 材料和方法部分: | pLR16-Phes* was a gift from Anat Herskovits (Addgene plasmid # 98783 ; http://n2t.net/addgene:98783 ; RRID:Addgene_98783) |
| 参考文献部分: | An Effective Counterselection System for Listeria monocytogenes and Its Use To Characterize the Monocin Genomic Region of Strain 10403S. Argov T, Rabinovich L, Sigal N, Herskovits AA. Appl Environ Microbiol. 2017 Mar 2;83(6). pii: e02927-16. doi: 10.1128/AEM.02927-16. Print |
买家导航
pLR16-Phes载体质粒基本信息
pLR16-Phes质粒图谱载体图谱和pLR16-Phes载体序列质粒序列多克隆位点信息
pLR16-Phes质粒载体简介
pLR16-Phes质粒序列载体序列
LOCUS pLR16-Phes 7703 bp DNA circular SYN 15-DEC-2025
DEFINITION Suicide vector for allelic exchange in Listeria utilizing an
engineered counterselection marker for Listeria monocytogenes based
on a mutated phenylalanyl-tRNA synthetase gene (pheS*)..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7703)
AUTHORS Argov T, Rabinovich L, Sigal N, Herskovits AA
TITLE An Effective Counterselection System for Listeria monocytogenes and
Its Use To Characterize the Monocin Genomic Region of Strain 10403S.
JOURNAL Appl Environ Microbiol. 2017 Mar 2;83(6). pii: e02927-16. doi:
10.1128/AEM.02927-16. Print 2017 Mar 15.
PUBMED 28039138
REFERENCE 2 (bases 1 to 7703)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl
Environ Microbiol."; date: "2017-03-2"; volume: "83(6). pii:
e02927-16. doi"; pages: " 10.1128/AEM.02927-16. Print 2017 Mar 15"
COMMENT Sequence Label: pLR16-Phes*
FEATURES Location/Qualifiers
source 1..7703
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(32..50)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind 138..154
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 139..155
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(189..205)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(189..205)
/label=pBluescriptSK
/note="For pBluescript vector"
oriT 1613..1722
/note="incP origin of transfer"
protein_bind 4257..4278
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(4293..4298,4299..4316,4317..4323)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4331..4347
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4336..4358
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4355..4371
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4355..4371
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS complement(4560..5219)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 5134..5153
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(5220..5322)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind 5550..5572
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6994..7011)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7128..7673)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(7208..7227)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tctagagcgg ccgccaccgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
61 aatgtcatga taataatggt ttcttagacg tcattaaccc tcactaaagg gaacaaaagc
121 tggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca
181 gcccggggga tccactagtt ctagagcggc cgccaccgat gtgaggcatt ttcgctcttt
241 ccggcgaggc tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt
301 gctttttcgt acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat
361 tatctcatat tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat
421 aaacaatcct gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa
481 atatattgaa ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt
541 actattatta ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag
601 agaaaaagca ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt
661 ctctacatca gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca
721 aataccagag aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc
781 ccaataacct aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat
841 cacccttgtc actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt
901 tcggtataaa acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa
961 ataatgatta aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat
1021 ttgatatgcc tcctaaattt ttatctaaag tgaatttagg aggcttactt gtctgctttc
1081 ttcattagaa tcaatccttt tttaaaagtc aatattactg taacataaat atatatttta
1141 aaaatatccc actttatcca attttcgttt gttgaactaa tgggtgcttt agttgaagaa
1201 taaaagacca cattaaaaaa tgtggtcttt tgtgtttttt taaaggattt gagcgtagcg
1261 aaaaatcctt ttctttctta tcttgataat aagggtaact attgccggga tagactgtaa
1321 cattctcacg cataaaatcc cctttcattt tctaatgtaa atctattacc ttattattaa
1381 ttcaattcgc tcataattaa tcctttttct tattacgcaa aatggcccga tttaagcaca
1441 ccctttattc cgttaatgcg ccatgacagc catgataatt actaatacta ggagaagtta
1501 ataaatacgt atcagtgcaa tttattcttc aaatgtagca cctgaagtca gccccatacg
1561 atataagttg taattctccg ccgcttgccc tcatctgtta cgccggcggt agccggccag
1621 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
1681 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
1741 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
1801 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaagcgc
1861 tgcttccctg ctgttttgtg gaatatctac cgactggaaa caggcaaatg caggaaatta
1921 ctgaactgag gggacaggcg agaggcgatc gcataacttc gtatagcata catgcataaa
1981 ctgcatccct taacttgttt ttcgtgtgcc tattttttgt gaatcgatta tgtcttttgc
2041 gcagtcggct taaaccagtt ttcgttggtg cgaaaaaaga gtgtcttgtg acactcttaa
2101 attcaaaatc tatcggtcag atttataccg atttgatttt atatattctt gaataacata
2161 cgccgagtta tcacataaaa gcgggaacca atcatcaaat ttaaacttca ttgcataatc
2221 cattaaactc ttaaattcta cgattccttg ttcatcaata aactcaatca tttctttaat
2281 taatttatat ctatctgttg ttgttttctt taataattca tcaacatcta caccgccata
2341 aactatcata tcttcttttt gatatttaaa tttattagga tcgtccatgt gaagcatata
2401 tctcacaaga cctttcacac ttcctgcaat ctgcggaata gtcgcattca attcttctgt
2461 aattattttt atctgttcat aagatttatt accctcatac atcactagaa tatgataatg
2521 ctcttttttc atcctatctt ctgtatcagt atccctatca tgtaatggag acactacaaa
2581 ttgaatgtgt aactctttta aatactctaa ccactcggct tttgctgatt ctggatataa
2641 aacaaatgtc caattacgtc ctcttgaatt tttcttgttt tcagtttctt ttattacatt
2701 ttcgctcatg atataataac ggtgctaata catttaacaa aatttagtca tagataggca
2761 gcatgccagt gctgtctatc tttttttgtt taaaatgcac cgtattcctc ctttgcatat
2821 ttttttatta gaataccggt tgcatctgat ttgctaatat tatatttttc tttgattcta
2881 tttaatatct cattttcttc tgttgtaagt cttaaagtaa cagcaacttt tttctcttct
2941 tttctatcta caaccatcac tgtacctccc aacatctgtt tttttcactt taacataaaa
3001 aacaaccttt taacattaaa aacccaatat ttatttattt gtttggacaa tggacaatgg
3061 acacctaggg gggaggtcgt agtacccccc tatgttttct cccctaaata accccaaaaa
3121 tctaagaaaa aaagacctca aaaaggtctt taattaacat ctcaaatttc gcatttattc
3181 caatttcctt tttgcgtgtg atgcgctgcg tccattaaaa atcctagagc tttgcaaccg
3241 aaagttaata gctgtcgcta ctactttcgc ttacgctcta agtatatttt aaggactgtc
3301 acacgcaaaa agttttctcg gcataaaagt acctctacat ctctaaatcg tctgtacgct
3361 gtttctcacg ctttctatcg accttctgga cattatcctg tacaacatcc ataaactgtc
3421 ccacacgctc gaatttggaa tcattaaaga atttctcttt aagcctatta aaccctttct
3481 caaacccagg gaaattcgcc ctcgcagcac gatataaagt cactgtacta tcttgaaatt
3541 tctctgatac attcaactgc tcattcaaac tatcattctc tcgctttaat ttattaacct
3601 ctttactttt ttcgtgatac ccctctttcc atgtattcac tacttctttc aaactctctc
3661 tacgtttttt taattcttga ttttctgtgt aatagtctgt gctcttaata ttttcgtaat
3721 catcaacaat ccgttcgaag agattgtttc ttgcaggcgt tcaaattcat cagcagttaa
3781 tatctttcta ccagtctctt cacgtccaga gaacaaacct gtacgctcat tttcataatc
3841 aaagggtttc gtagacctca tatgctctat tccactctgt aactgcttat ttgccttctg
3901 taactcatcc ttaacttctt gcagttcctg tttatgaaat acagtatctt tcttgtactg
3961 atccagttgc tcaaaaaaat ctcggtcaga tgttactagc aactcattta caagaacagc
4021 atctttcctc gtttttcttg tacctgtttt ttgtgattca ataatttctt tgacacgttc
4081 gttgtaatca atatttttat catttttcaa atcataattt tcacgtgttc gctcatggtc
4141 aatatcatca ttcgttctac tttttcgctc tctttgatta tgaaattgca tgccttttag
4201 tccagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
4261 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
4321 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
4381 aattcgagct ccaattcgcc ctatagtgag tcgtattgac gtcgctattt aacgaccctg
4441 ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta
4501 tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc ttaaaaaaat
4561 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
4621 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
4681 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
4741 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
4801 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
4861 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
4921 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
4981 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga
5041 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
5101 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
5161 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
5221 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
5281 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa
5341 aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag
5401 tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta
5461 ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg
5521 tccctcctgt tcagtactga cggggtggtc cgggagctgc atgtgtcaga ggttttcacc
5581 gtcatcaccg aaacgacctt acgcctgttt aaactgcgaa ataaatctga catcgtttgt
5641 atagaagtgg cggatatcat caatgccata tttcagcatc gcgatgcgct caacacccat
5701 tccgaatccg aagccctgat attccttcgg atcaaagcca gccattttaa gcacgttcgg
5761 gtgaaccatt ccggcaccga ggatttcaat ccagcctgtt cctttacata ctgagcagcc
5821 gttcccaccg catttaaagc atgtcacatc cacttctact gaaggctcag taaacgggaa
5881 gaagcttggg cggagtctga tttcacggtc ttgcccgaac atttttttcg caacaagttc
5941 aagcgttcct tttaaatcac tcatgctgat gtttttgtca acgacaagcc cttcaatttg
6001 cataaattgg tgagagtgcg tcgcatcatc gttatcacgg cgatatactt tacccgggca
6061 aatgatttta acgggacctt tgccttcatg cttttccatc gtacgtgttt ggacaggaga
6121 agtttgcgtt ctcatcaaag tttcctctgt gatgtaaaag ctgtcctgca tatcgcgcgc
6181 tgggtgttct ttcggaagat tgagcgattc gaagttgtag taatccgttt caacctctgg
6241 cccttcctcg actgtgtagc ccataccgat aaataaatct tcaatttctt caatgacaac
6301 agtgagcgga tggcggccgc cgactgcaac agggttcccc ggcagcgtga cgtcaattgt
6361 ctgtcctgca agcttctgtt tcatttcctc ttcttcaagc ttctcgtttt tgtcagcaat
6421 cgcattggca atacgctccc ttacttcgtt cgcgagcgcc cccatttttg gacgttcctc
6481 agcagaaagc ttgcccattc cgcgcagcac ttctgtaatc ggcccttttt ttccgagata
6541 ttgcacccga atatcattga caaccttcaa tgagcttgcc gcttctactt gttctaaagc
6601 ttcttgttcc agctgtttta gcttttcttc catctgcagc actctccttc tacatttttt
6661 aacctaataa tgccaaatac cgtttgccac ccctctcttt tgataattat aatattggcg
6721 aaattcgctt ctaaagatga aacgcaatat tatatgcttg ctttatcggc cgacacacat
6781 tatgccacac cttgtagata aagtcaacaa ctttttgcaa aattgagctc gcgtaacggc
6841 aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg
6901 atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca
6961 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt
7021 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc
7081 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc
7141 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
7201 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
7261 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
7321 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
7381 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
7441 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
7501 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
7561 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
7621 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
7681 atctattaat cagataaaat att
//
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