| 载体名称: | pXC17.4 |
|---|---|
| 载体又名: | pXC17.4载体，pXC17.4质粒，pXC17.4载体质粒 |
| 英文名称: | pXC17.4, pXC17.4 Vector, pXC17.4 Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | mCMV |
| 载体大小: | 6.9 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | GS加压筛选 |
| 克隆菌株: | DH5α,TOP10 等 |
| 备注: | 用于ChOK1SV等细胞的GS筛选哺乳细胞蛋白表达。 |
| 产品目录号: | 212603 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 相关载体: | pXC18.4 |
pXC17.4
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pXC17.4载体质粒基本信息
pXC17.4质粒图谱载体图谱和pXC17.4载体序列质粒序列多克隆位点信息
pXC17.4质粒载体简介
pXC17.4质粒序列载体序列
Snapgene载体序列下载: pXC17.4序列 .gb格式载体序列下载:pXC17.4载体序列
LOCUS pXC17.4 6934 bp DNA circular SYN 19-JUN-2026
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6934)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jun 19, 2026 from SnapGene 8.1.0
https://www.biofeng.com
COMMENT Sequence Label: pXC17.4
FEATURES Location/Qualifiers
source 1..6934
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 116..237
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
rep_origin complement(999..1587)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(join(1758..2549,2550..2618))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 1758 .. 2549 / #ccffcc
2: 2550 .. 2618 / #ccffcc / signal sequence
Cleavage site after base 2549"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2619..2723)
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: LEFT"
terminator 2892..2919
/label=T7Te terminator
/note="phage T7 early transcription terminator"
/note="color: #ffffff; direction: RIGHT"
promoter 3002..3331
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
/note="color: #ffffff; direction: RIGHT"
rep_origin 3182..3317
/label=SV40 ori
/note="SV40 origin of replication"
/note="color: #ffff00"
CDS 3370..4488
/codon_start=1
/label=Glutamine Synthetase
/note="color: #993366"
/translation="MATSASSHLNKNIKQMYLCLPQGEKVQAMYIWVDGTGEGLRCKTR
TLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRRDPNKLVFCEVFKY
NRKPAETNLRHSCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYC
GVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR
FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKHIEEAIEKLSKRH
RYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDR
RPSANCDPFAVTEAIVRTCLLNETGDEPFQYKN"
intron 4623..4688
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
/note="color: #------"
CDS 4818..4838
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/note="color: #993366"
/translation="PKKKRKV"
polyA_signal 5263..5397
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
promoter 5422..5956
/label=mCMV Promoter
/note="color: #ffffff; direction: RIGHT"
intron 6050..6876
/label=CMV intron A
/note="human cytomegalovirus intron A (Chapman et al.,
1991)"
/note="color: #------"
ORIGIN
1 gaattcattg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
61 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
241 tgtctggcgg ccgcgacctg caggcgcaga actggtaggt atggaagatc cctcgagatc
301 cattgtgctg gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa
361 atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg
421 cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct
481 gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt
541 tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca
601 ctttatcact gataaacata atatgtccac caacttatca gtgataaaga atccgcgcca
661 gcacaatgga tctcgaggtc gagggatctc tagaggatcc tctacgccgg acgcatcgtg
721 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
781 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
841 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
901 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
961 aagggagagc gtcgacctcg ggccgcgttg ctggcgtttt tccataggct ccgcccccct
1021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
1081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
1141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
1201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
1261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
1321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
1381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
1441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
1501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
1561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
1621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
1681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
1741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
1801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
1861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
1921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
1981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
2041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
2101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
2161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
2221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
2281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
2341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
2401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
2461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
2521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
2581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
2641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
2701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
2761 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctgatggctc
2821 tttgcggcac ccatcgttcg taatgttccg tggcaccgag gacaaccctc aagagaaaat
2881 gtaatcacac tggctcacct tcgggtgggc ctttctgcgt ttataaggag acactttatg
2941 tttaagaagg ttggtaaatt ccttgcggct ttggcagcca agctagatcc ggctgtggaa
3001 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
3061 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
3121 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
3181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
3241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
3301 aggctttttt ggaggcctag gcttttgcaa aaagctagct tggggccacc gctcagagca
3361 ccttccacca tggccacctc agcaagttcc cacttgaaca aaaacatcaa gcaaatgtac
3421 ttgtgcctgc cccagggtga gaaagtccaa gccatgtata tctgggttga tggtactgga
3481 gaaggactgc gctgcaaaac ccgcaccctg gactgtgagc ccaagtgtgt agaagagtta
3541 cctgagtgga attttgatgg ctctagtacc tttcagtctg agggctccaa cagtgacatg
3601 tatctcagcc ctgttgccat gtttcgggac cccttccgca gagatcccaa caagctggtg
3661 ttctgtgaag ttttcaagta caaccggaag cctgcagaga ccaatttaag gcactcgtgt
3721 aaacggataa tggacatggt gagcaaccag cacccctggt ttggaatgga acaggagtat
3781 actctgatgg gaacagatgg gcaccctttt ggttggcctt ccaatggctt tcctgggccc
3841 caaggtccgt attactgtgg tgtgggcgca gacaaagcct atggcaggga tatcgtggag
3901 gctcactacc gcgcctgctt gtatgctggg gtcaagatta caggaacaaa tgctgaggtc
3961 atgcctgccc agtgggagtt ccaaatagga ccctgtgaag gaatccgcat gggagatcat
4021 ctctgggtgg cccgtttcat cttgcatcga gtatgtgaag actttggggt aatagcaacc
4081 tttgacccca agcccattcc tgggaactgg aatggtgcag gctgccatac caactttagc
4141 accaaggcca tgcgggagga gaatggtctg aagcacatcg aggaggccat cgagaaacta
4201 agcaagcggc accggtacca cattcgagcc tacgatccca aggggggcct ggacaatgcc
4261 cgtcgtctga ctgggttcca cgaaacgtcc aacatcaacg acttttctgc tggtgtcgcc
4321 aatcgcagtg ccagcatccg cattccccgg actgtcggcc aggagaagaa aggttacttt
4381 gaagaccgcc gcccctctgc caattgtgac ccctttgcag tgacagaagc catcgtccgc
4441 acatgccttc tcaatgagac tggcgacgag cccttccaat acaaaaacta attagacttt
4501 gagtgatctt gagcctttcc tagttcatcc caccccgccc cagagagatc tttgtgaagg
4561 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
4621 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
4681 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
4741 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
4801 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
4861 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
4921 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
4981 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
5041 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
5101 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
5161 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
5221 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
5281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
5341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctct
5401 agcttcgtgt caaggacggt gaggcgcgcc tactgagtca ttagggactt tccaatgggt
5461 tttgcccagt acataaggtc aataggggtg aatcaacagg aaagtcccat tggagccaag
5521 tacactgagt caatagggac tttccattgg gttttgccca gtacaaaagg tcaatagggg
5581 gtgagtcaat gggtttttcc cattattggc acgtacataa ggtcaatagg ggtgagtcat
5641 tgggtttttc cagccaattt aattaaaacg ccatgtactt tcccaccatt gacgtcaatg
5701 ggctattgaa actaatgcaa cgtgaccttt aaacggtact ttcccatagc tgattaatgg
5761 gaaagtaccg ttctcgagcc aatacacgtc aatgggaagt gaaagggcag ccaaaacgta
5821 acaccgcccc ggttttcccc tggaaattcc atattggcac gcattctatt ggctgagctg
5881 cgttctacgt gggtataaga ggcgcgacca gcgtcggtac cgtcgcagtc ttcggtctga
5941 ccaccgtaga acgcagcctc aggacctcca tagaagacac cgggaccgat ccagcctccg
6001 cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc
6061 ctatagagtc tataggccca cccccttggc ttcttatgca tgctatactg tttttggctt
6121 ggggtctata cacccccgct tcctcatgtt ataggtgatg gtatagctta gcctataggt
6181 gtgggttatt gaccattatt gaccactccc ctattggtga cgatactttc cattactaat
6241 ccataacatg gctctttgcc acaactctct ttattggcta tatgccaata cactgtcctt
6301 cagagactga cacggactct gtatttttac aggatggggt ctcatttatt atttacaaat
6361 tcacatatac aacaccaccg tccccagtgc ccgcagtttt tattaaacat aacgtgggat
6421 ctccacgcga atctcgggta cgtgttccgg acatgggctc ttctccggta gcggcggagc
6481 ttctacatcc gagccctgct cccatgcctc cagcgactca tggtcgctcg gcagctcctt
6541 gctcctaaca gtggaggcca gacttaggca cagcacgatg cccaccacca ccagtgtgcc
6601 gcacaaggcc gtggcggtag ggtatgtgtc tgaaaatgag ctcggggagc gggcttgcac
6661 cgctgacgca tttggaagac ttaaggcagc ggcagaagaa gatgcaggca gctgagttgt
6721 tgtgttctga taagagtcag aggtaactcc cgttgcggtg ctgttaacgg tggagggcag
6781 tgtagtctga gcagtactcg ttgctgccgc gcgcgccacc agacataata gctgacagac
6841 taacagactg ttcctttcca tgggtctttt ctgcagtcac cgtccttgac acgaagcttc
6901 gcgacgtacg ttcgaacccg ggcacgtgcg gacc
//
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