pSL468

LOCUS       pSL468                8291 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..900
                     /label=NheI
     misc_feature    911..915
                     /label=New Feature
     misc_feature    911..915
                     /label=New Feature
     misc_feature    926..2485
                     /label=SEAP
     primer_bind     2417..2441
                     /label=183-WKG-599-F
     polyA_signal    2477..2485
                     /label=SV40 late polyA
     misc_feature    2511..2514
                     /label=linker
     misc_feature    2531..2543
                     /label=linker
     misc_feature    2549..2567
                     /label=MS2 box
     misc_feature    2573..2582
                     /label=linker
     misc_feature    2588..2606
                     /label=MS2 box
     misc_feature    2612..2621
                     /label=linker
     misc_feature    2612..2621
                     /label=linker
     misc_feature    2620..2650
                     /label=hairpin
     misc_feature    2626..2644
                     /label=MS2 box
     misc_feature    2649..2661
                     /label=linker
     misc_feature    2660..2690
                     /label=hairpin
     misc_feature    2666..2684
                     /label=MS2 box
     misc_feature    2694..2699
                     /label=linker
     misc_feature    2708..2723
                     /label=linker
     misc_feature    2729..2732
                     /label=linker
     misc_feature    2749..2761
                     /label=linker
     misc_feature    2767..2785
                     /label=MS2 box
     misc_feature    2791..2800
                     /label=linker
     misc_feature    2806..2824
                     /label=MS2 box
     misc_feature    2830..2839
                     /label=linker
     misc_feature    2830..2839
                     /label=linker
     misc_feature    2838..2868
                     /label=hairpin
     misc_feature    2844..2862
                     /label=MS2 box
     misc_feature    2867..2879
                     /label=linker
     misc_feature    2878..2908
                     /label=hairpin
     misc_feature    2884..2902
                     /label=MS2 box
     misc_feature    2912..2917
                     /label=linker
     misc_feature    2926..2941
                     /label=linker
     misc_feature    2947..2950
                     /label=linker
     misc_feature    2967..2979
                     /label=linker
     misc_feature    2985..3003
                     /label=MS2 box
     misc_feature    3009..3018
                     /label=linker
     misc_feature    3024..3042
                     /label=MS2 box
     misc_feature    3048..3057
                     /label=linker
     misc_feature    3048..3057
                     /label=linker
     misc_feature    3056..3086
                     /label=hairpin
     misc_feature    3062..3080
                     /label=MS2 box
     misc_feature    3085..3097
                     /label=linker
     misc_feature    3096..3126
                     /label=hairpin
     misc_feature    3102..3120
                     /label=MS2 box
     misc_feature    3130..3135
                     /label=linker
     misc_feature    3144..3159
                     /label=linker
     misc_feature    3186..3198
                     /label=linker
     misc_feature    3204..3222
                     /label=MS2 box
     misc_feature    3228..3237
                     /label=linker
     misc_feature    3243..3261
                     /label=MS2 box
     misc_feature    3267..3276
                     /label=linker
     misc_feature    3267..3276
                     /label=linker
     misc_feature    3275..3305
                     /label=hairpin
     misc_feature    3281..3299
                     /label=MS2 box
     misc_feature    3304..3316
                     /label=linker
     misc_feature    3315..3345
                     /label=hairpin
     misc_feature    3321..3339
                     /label=MS2 box
     misc_feature    3349..3354
                     /label=linker
     misc_feature    3363..3378
                     /label=linker
     misc_feature    3384..3396
                     /label=linker
     misc_feature    3402..3420
                     /label=MS2 box
     misc_feature    3426..3435
                     /label=linker
     misc_feature    3441..3459
                     /label=MS2 box
     misc_feature    3465..3474
                     /label=linker
     misc_feature    3465..3474
                     /label=linker
     misc_feature    3473..3503
                     /label=hairpin
     misc_feature    3479..3497
                     /label=MS2 box
     misc_feature    3502..3514
                     /label=linker
     misc_feature    3513..3543
                     /label=hairpin
     misc_feature    3519..3537
                     /label=MS2 box
     misc_feature    3547..3552
                     /label=linker
     misc_feature    3561..3576
                     /label=linker
     misc_feature    3582..3594
                     /label=linker
     misc_feature    3600..3618
                     /label=MS2 box
     misc_feature    3624..3633
                     /label=linker
     misc_feature    3639..3657
                     /label=MS2 box
     misc_feature    3663..3672
                     /label=linker
     misc_feature    3663..3672
                     /label=linker
     misc_feature    3671..3701
                     /label=hairpin
     misc_feature    3677..3695
                     /label=MS2 box
     misc_feature    3700..3712
                     /label=linker
     misc_feature    3711..3741
                     /label=hairpin
     misc_feature    3717..3735
                     /label=MS2 box
     misc_feature    3745..3750
                     /label=linker
     misc_feature    3759..3774
                     /label=linker
     misc_feature    3781..3856
                     /label=HHR Env140_nutR_H6
     misc_feature    complement(3789..3797)
                     /label=Stem III
     misc_feature    3800..3815
                     /label=Stem I
     terminator      3824..3838
                     /label=nutR-boxB
     misc_feature    3842..3850
                     /label=Stem III
     polyA_signal    3888..4112
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4158..4586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4172..4196)
                     /label=184-WKG-599-R
     primer_bind     4457..4476
                     /label=189-WKG-612-F
     promoter        4600..4929
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4996..5790
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5040..5067)
                     /label=188-WKG-610-R
     polyA_signal    5964..6085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6130..6150)
                     /label=M13-rev
     primer_bind     complement(6134..6150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6156..6178)
                     /label=LacO
     protein_bind    6158..6174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6182..6212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(6183..6212)
                     /label=lac
     protein_bind    6227..6248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6354..7294
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7295..8155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8156..8260)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttagcg ccaccatgct gctgctgctg ctgctgctgg gcctgaggct
      961 acagctctcc ctgggcatca tcccagttga ggaggagaac ccggacttct ggaaccgcga
     1021 ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa
     1081 cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct
     1141 aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc
     1201 atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac
     1261 agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc
     1321 cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg
     1381 ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc
     1441 gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc
     1501 tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga
     1561 cattgacgtg atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc
     1621 tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca
     1681 ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca
     1741 ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa
     1801 atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc
     1861 cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat
     1921 cgaccatggt catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga
     1981 cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac
     2041 tgccgaccac tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt
     2101 cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa
     2161 cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg
     2221 gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga
     2281 ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca
     2341 gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
     2401 cctggcgccc cccgccggca ccaccgacgc cgcgcacccg ggttactcta gagtcggggc
     2461 ggccggccgc ttcgagcaga catgagcggc cgctcaatct cgagggatcc aacttgaacc
     2521 ggtaagaatt gcaacaaaca aatcaccacc gtgaggatca cccacggtgg tgaaagcctt
     2581 agcctagccg tgaggatcac ccacggtagg cacaagaaat tctacccgtg aggatcaccc
     2641 acgggtagaa tcacaaagac tacagccgtg aggatcaccc acggctgtag actcaaactt
     2701 aaaaaggata tcttcagcaa actgatccaa cttgaaccgg taagaattgc aacaaacaaa
     2761 tcaccaccgt gaggatcacc cacggtggtg aaagccttag cctagccgtg aggatcaccc
     2821 acggtaggca caagaaattc tacccgtgag gatcacccac gggtagaatc acaaagacta
     2881 cagccgtgag gatcacccac ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac
     2941 tgatccaact tgaaccggta agaattgcaa caaacaaatc accaccgtga ggatcaccca
     3001 cggtggtgaa agccttagcc tagccgtgag gatcacccac ggtaggcaca agaaattcta
     3061 cccgtgagga tcacccacgg gtagaatcac aaagactaca gccgtgagga tcacccacgg
     3121 ctgtagactc aaacttaaaa aggatatctt cagcaaactg atcggagacg agtccgtctc
     3181 aaattgcaac aaacaaatca ccaccgtgag gatcacccac ggtggtgaaa gccttagcct
     3241 agccgtgagg atcacccacg gtaggcacaa gaaattctac ccgtgaggat cacccacggg
     3301 tagaatcaca aagactacag ccgtgaggat cacccacggc tgtagactca aacttaaaaa
     3361 ggatatcttc agcaaactga attgcaacaa acaaatcacc accgtgagga tcacccacgg
     3421 tggtgaaagc cttagcctag ccgtgaggat cacccacggt aggcacaaga aattctaccc
     3481 gtgaggatca cccacgggta gaatcacaaa gactacagcc gtgaggatca cccacggctg
     3541 tagactcaaa cttaaaaagg atatcttcag caaactgaat tgcaacaaac aaatcaccac
     3601 cgtgaggatc acccacggtg gtgaaagcct tagcctagcc gtgaggatca cccacggtag
     3661 gcacaagaaa ttctacccgt gaggatcacc cacgggtaga atcacaaaga ctacagccgt
     3721 gaggatcacc cacggctgta gactcaaact taaaaaggat atcttcagca aactgaattc
     3781 gtttaaaccc tcctcgtccg gggctgtgtc gccccgctga cgagccctga aaaagggcga
     3841 aacgaggagg actagtgggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     3901 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     3961 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4021 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4081 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4141 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4201 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4261 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4321 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4381 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4441 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4501 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4561 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     4621 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4681 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4741 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4801 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4861 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4921 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     4981 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5041 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5101 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5161 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5221 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5281 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5341 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5401 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5461 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5521 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5581 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5641 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5701 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5761 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     5821 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     5881 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5941 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6061 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6121 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8281 cacctgacgt c
//