pSL893

LOCUS       pSL893           8147 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..191
                     /label=182-WKG-596-F
     misc_feature    232
                     /label=CMV
     promoter        245..441
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    473..535
                     /label=Igk secretion signal
     CDS             541
                     /codon_start=1
                     /label=linker
                     /translation=""
     CDS             542..1240
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    1247..1990
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     primer_bind     complement(1314..1336)
                     /label=186-WKG-603-R
     misc_feature    1520..1522
                     /label=F93A
                     /note="F93A"
     misc_feature    1968..1990
                     /label=EpoR-D1_D2_TM
                     /note="EpoR-D1_D2_TM"
     misc_feature    1968..1990
                     /label=transmembrane
                     /note="transmembrane"
     misc_feature    1991..3703
                     /label=VEGFR
                     /note="VEGFR"
     polyA_signal    3744..3968
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4014..4442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4313..4332
                     /label=189-WKG-612-F
     promoter        4456..4785
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4852..5646
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4896..4923)
                     /label=188-WKG-610-R
     polyA_signal    5820..5941
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5986..6006)
                     /label=M13-rev
     primer_bind     complement(5990..6006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(6012..6034)
                     /label=LacO
     protein_bind    6014..6030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6038..6068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(6039..6068)
                     /label=lac
     protein_bind    6083..6104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6210..7150
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(7151..8011)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8012..8116)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgatctgc
      241 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      301 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
      361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      421 ggaggcctag gcttttgcaa aaagctacga atcgcgctag tggccggcca ccatggagac
      481 cgatacctta ttactgtggg tgcttcttct gtgggttccc ggtagcaccg gagacggatc
      541 cgacatcgag aagctgtgca agaaggcgga agaggaggcc aaggaggccc aggagaaggc
      601 cgacgagctg agacaacgac accccgattc gcaggcggcg gaagacgcgg aggatctcgc
      661 aaatctggct gtcgccgccg tgctgaccgc ttgtctgctg gcccaggagc atcccaacgc
      721 cgatatcgct aagctgtgca tcaaggccgc gagcgaagct gcggaggccg cgtcaaaagc
      781 cgccgagctg gcccaacggc acccagacag tcaagcagcc agagatgcta tcaaactcgc
      841 ctctcaagcc gcccgcgccg tgatcctggc catcatgctc gccgcagaga atcccaacgc
      901 tgatattgca aagctatgta ttaaggcggc cagcgaagcc gcagaggctg cctcgaaagc
      961 tgcggaactt gcacagcggc accccgactc ccaggccgcc cgagatgcaa ttaaactggc
     1021 cagtcaggcc gctgaggcgg tggagagagc catctggctg gccgccgaga accccaatgc
     1081 cgatatcgcc aaaaaatgca tcaaagcggc ctccgaagca gccgaggagg cctctaaagc
     1141 tgccgaagag gctcaaagac acccagacag ccagaaggca cgcgacgaga tcaaggaggc
     1201 ctctcagaag gccgaggagg tgaaggagag atgcaagagc gaattcgcac cttcacccag
     1261 cctcccggac cccaagtttg agagcaaagc ggccctgctg gcatcccggg gctccgaaga
     1321 acttctgtgc ttcacccaac gcttggaaga cttggtgtgt ttctgggagg aagcggcgag
     1381 ctccgggatg gacttcaact acagcttctc ataccagctc gagggtgagt cacgaaagtc
     1441 atgtagcctg caccaggctc ccaccgtccg cggctccgtg cgtttctggt gttcactgcc
     1501 aacagcggac acatcgagtg ctgtgccgct ggagctgcag gtgacggagg cgtccggttc
     1561 tcctcgctat caccgcatca tccatatcaa tgaagtagtg ctcctggacg cccccgcggg
     1621 gctgctggcg cgccgggcag aagagggcag ccacgtggtg ctgcgctggc tgccacctcc
     1681 tggagcacct atgaccaccc acatccgata tgaagtggac gtgtcggcag gcaaccgggc
     1741 aggagggaca caaagggtgg aggtcctgga aggccgcact gagtgtgttc tgagcaacct
     1801 gcggggcggg acgcgctaca ccttcgctgt tcgagcgcgc atggccgagc cgagcttcag
     1861 cggattctgg agtgcctggt ctgagcccgc gtcactactg accgctagcg acctggaccc
     1921 tctcatcttg acgctgtctc tcattctggt cctcatctcg ttgttgctga cggttctggc
     1981 cctgctgtcc ctacggaccg ttaagcgggc caatggaggg gaactgaaga caggctactt
     2041 gtccatcgtc atggacccag atgaactccc attggatgaa cattgtgaac gactgcctta
     2101 tgatgccagc aaatgggagt tccccagaga ccggctgaag ctaggtaagc ctcttggccg
     2161 tggtgccttt ggccaagtga ttgaagcaga tgcctttgga attgacaaga cagcaacttg
     2221 caggacagta gcagtcaaaa tgttgaaaga aggagcaaca cacagtgagc atcgagctct
     2281 catgtctgaa ctcaagatcc tcattcatat tggtcaccat ctcaatgtgg tcaaccttct
     2341 aggtgcctgt accaagccag gagggccact catggtgatt gtggagttct gcaaatttgg
     2401 aaacctgtcc acttacctga ggagcaagag aaatgaattt gtcccctaca agaccaaagg
     2461 ggcacgattc cgtcaaggga aagactacgt tggagcaatc cctgtggatc tgaaacggcg
     2521 cttggacagc atcaccagta gccagagctc agccagctct ggatttgtgg aggagaagtc
     2581 cctcagtgat gtagaagaag aggaagctcc tgaagatctg tataaggact tcctgacctt
     2641 ggagcatctc atctgttaca gcttccaagt ggctaagggc atggagttct tggcatcgcg
     2701 aaagtgtatc cacagggacc tggcggcacg aaatatcctc ttatcggaga agaacgtggt
     2761 taaaatctgt gactttggct tggcccggga tatttataaa gatccagatt atgtcagaaa
     2821 aggagatgct cgcctccctt tgaaatggat ggccccagaa acaatttttg acagagtgta
     2881 cacaatccag agtgacgtct ggtcttttgg tgttttgctg tgggaaatat tttccttagg
     2941 tgcttctcca tatcctgggg taaagattga tgaagaattt tgtaggcgat tgaaagaagg
     3001 aactagaatg agggcccctg attatactac accagaaatg taccagacca tgctggactg
     3061 ctggcacggg gagcccagtc agagacccac gttttcagag ttggtggaac atttgggaaa
     3121 tctcttgcaa gctaatgctc agcaggatgg caaagactac attgttcttc cgatatcaga
     3181 gactttgagc atggaagagg attctggact ctctctgcct acctcacctg tttcctgtat
     3241 ggaggaggag gaagtatgtg accccaaatt ccattatgac aacacagcag gaatcagtca
     3301 gtatctgcag aacagtaagc gaaagagccg gcctgtgagt gtaaaaacat ttgaagatat
     3361 cccgttagaa gaaccagaag taaaagtaat cccagatgac aaccagacgg acagtggtat
     3421 ggttcttgcc tcagaagagc tgaaaacttt ggaagacaga accaaattat ctccatcttt
     3481 tggtggaatg gtgcccagca aaagcaggga gtctgtggca tctgaaggct caaaccagac
     3541 aagcggctac cagtccggat atcactccga tgacacagac accaccgtgt actccagtga
     3601 ggaagcagaa cttttaaagc tgatagagat tggagtgcaa accggtagca cagcccagat
     3661 tctccagcct gactcgggga ccacactgag ctctcctcct gtttaatcta gagggcccgt
     3721 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3781 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3841 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3901 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     3961 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4021 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4081 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4141 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4201 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4261 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4321 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4381 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4441 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4501 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4561 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4621 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4681 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4741 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4801 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4861 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4921 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4981 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5041 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5101 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5161 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5221 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5281 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5341 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5401 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5461 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5521 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5581 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5641 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5701 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5761 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5821 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5881 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5941 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6001 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6061 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6121 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6181 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6241 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6301 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6361 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6421 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6481 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6541 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6601 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6661 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6721 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6781 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6841 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6901 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6961 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7021 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7081 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7141 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7201 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7261 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7321 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7381 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7441 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7501 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7561 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7621 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7681 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7741 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7801 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7861 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7921 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7981 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8041 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8101 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//