| 质粒名称: | pLenti6.2-ccdB-3xFLAG-V5 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 87071 |
| 存储实验室: | Susan Lindquist, Mikko Taipale |
|
载体骨架基本信息 |
|
| 载体名称: | Lenti6.2 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Blasticidin |
| 载体抗性: | Chloramphenicol and Ampicillin, 25 & 100 μg/mL |
| 生长温度: | 30℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 高拷贝 |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti6.2-ccdB-3xFLAG-V5 was a gift from Susan Lindquist & Mikko Taipale (Addgene plasmid # 87071 ; http://n2t.net/addgene:87071 ; RRID:Addgene_87071) |
| 参考文献部分: | A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways. Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, Choi H, Berger B, Gingras AC, Lindquist S. Cell. 2014 Jul 17;158(2):434-48. doi: 10.1016/j.cell.2014.05.039. 10.1016/j.cell.2014.05.039 PubMed 25036637 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLenti6.2-ccdB-3xFLAG-V5 9022 bp ds-DNA circular SYN 19-JAN-2022
DEFINITION Lentiviral Gateway-compatible expression vector backbone with a
C-terminal 3xFLAG-V5 tag.
ACCESSION .
VERSION .
KEYWORDS pLenti6.2-ccdB-3xFLAG-V5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9022)
AUTHORS Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, Choi H,
Berger B, Gingras AC, Lindquist S
TITLE A quantitative chaperone interaction network reveals the
architecture of cellular protein homeostasis pathways.
JOURNAL Cell. 2014 Jul 17;158(2):434-48. doi: 10.1016/j.cell.2014.05.039.
PUBMED 25036637
REFERENCE 2 (bases 1 to 9022)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 19, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9022
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 239..258
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
promoter 320..367
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 386..784
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
LTR 871..1104
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 1176..1297
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1213..1232)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1267..1286
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 1337..1472
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 1399..1418
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter complement(1493..1511)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1521..1538)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(1521..1537)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1530..1552)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 1679..2134
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1766..1785)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1976..1997
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 2160..2264
/gene="bla"
/label=AmpR promoter
CDS 2265..3125
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2483..2502)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3296..3884
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3785..3804
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4038..4055
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 4172..4193
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4208..4238
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4246..4262
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4251..4273
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4270..4286
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4270..4286
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 4305..4325
/label=T3
/note="T3 promoter, forward primer"
promoter 4307..4325
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 4353..4579
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 4580..4760
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4807..4932
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5425..5658
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 5843..5887
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
enhancer 6232..6534
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 6535..6738
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 6689..6709
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
promoter 6783..6801
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 6889..7013
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 7038..7068
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 7122..7781
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(7188..7207)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 8123..8428
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 8307..8326
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(8469..8593)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 8595..8660
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 8679..8720
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 8755..8796
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
promoter 8829..306
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(8864..8887)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
ORIGIN
1 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
61 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
121 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
181 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
241 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
301 ctttcgactc tagacacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
361 atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag aatccaccct
421 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
481 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
541 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
601 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
661 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
721 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
781 ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
841 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
901 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
1021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
1081 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
1141 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
1201 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
1261 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
1321 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
1381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
1441 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
1501 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
1561 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
1621 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
1681 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
1741 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
1801 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
1861 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
1921 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
1981 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
2041 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
2101 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
2161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
2221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
2281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
2341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
2401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
2461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
2521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
2581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
2641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
2701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
2761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
2821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
2881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
2941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
3001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
3061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
3121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
3181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
3241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
3301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
3361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
3421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
3481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
3541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
3601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
3661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
3721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
3781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
3841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
3901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
3961 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
4021 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
4081 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
4141 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
4201 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
4261 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
4321 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
4381 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
4441 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
4501 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
4561 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
4621 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
4681 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
4741 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
4801 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
4861 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
4921 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
4981 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
5041 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
5101 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
5161 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
5221 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
5281 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
5341 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
5401 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
5461 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
5521 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
5581 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
5641 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
5701 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
5761 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
5821 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
5881 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
5941 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
6001 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
6061 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
6121 attagtgaac ggatctcgac ggtatcgatg tcgacgttaa cgctagcgac tgggtctgcg
6181 atgtcgacgt taacgctagc gactgggtct gcgataagct tgggagttcc gcgttacata
6241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
6301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
6361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
6421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
6481 atgggacttt cctacttggc agtacatcta gtattagtca tcgctattac catggtgatg
6541 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
6601 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
6661 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
6721 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
6781 attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta
6841 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
6901 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
6961 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
7021 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
7081 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
7141 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
7201 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
7261 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
7321 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
7381 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
7441 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
7501 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
7561 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
7621 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
7681 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
7741 aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac
7801 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
7861 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
7921 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
7981 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
8041 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
8101 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
8161 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
8221 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
8281 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
8341 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
8401 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
8461 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
8521 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
8581 tgtacaaagt ggttgattac aaagaccatg acggtgatta taaagatcat gacatcgact
8641 acaaggatga cgatgacaag ggctcgggct cgacctcggg taagcctatc cctaaccctc
8701 tcctcggtct cgattctaca tgataactcg agtctagagg gcccgcggtt cgaaggtaag
8761 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgacgatgg
8821 tacctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
8881 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
8941 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
9001 cctagtcagg aagttccccc cc
//
