| 质粒名称: | pHRW40 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 130973 |
| 存储实验室: | Chris Todd Hittinger |
|
载体骨架基本信息 |
|
| 载体名称: | pHCT2 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达 |
| 筛选标记: | 新霉素（G418）,G418 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | empty backbone |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHRW40 was a gift from Chris Todd Hittinger (Addgene plasmid # 130973 ; http://n2t.net/addgene:130973 ; RRID:Addgene_130973) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHRW40 11218 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION HyPr plasmid with nptII marker.
ACCESSION .
VERSION .
KEYWORDS pHRW40
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11218)
TITLE Allododecaploid yeasts: synthetic hybrids of six species
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11218)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11218
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 1..19
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 43..61
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 85..103
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 127..145
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 169..187
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 211..229
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 1569..1580
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 2226..2723
/codon_start=1
/product="13 tandem Myc epitope tags"
/label=13xMyc
/translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
EDL"
terminator 2799..3046
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(2815..2833)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
primer_bind complement(3069..3085)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3069..3085)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3082..3104)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3093..3109
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3117..3147)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3162..3183
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3300..3317)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3471..4059)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3551..3570)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4230..5090)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4853..4872
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5091..5195)
/gene="bla"
/label=AmpR promoter
primer_bind 5263..5281
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
gene 5403..6759
/label=kanMX
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 5403..5746
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 5747..6556
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(5814..5833)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
terminator 6562..6759
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
rep_origin 7102..7939
/label=ARS1
/note="S. cerevisiae autonomously replicating sequence
ARS1/ARS416"
misc_feature 8075..8367
/label=CEN4
/note="S. cerevisiae CEN4 centromere"
primer_bind 9109..9131
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9123..9140
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 9124..9140
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(9168..10175)
/codon_start=1
/product="tetracycline-controlled transactivator,
comprising a fusion of the tetracycline repressor TetR with
the C-terminal activation domain of herpes simplex virus
VP16"
/label=tTA
/note="In the Tet-Off(R) system, tTA binds to the
Tet-responsive element and stimulates transcription only in
the absence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
primer_bind 10083..10102
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
primer_bind complement(10260..10284)
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter complement(10281..10484)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(10310..10330)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 10485..10864
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind complement(11029..11053)
/label=pHybLex-R
/note="ADH terminator, reverse primer"
ORIGIN
1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
241 cgagctcggt accctatggc atgcatgtgc tctgtatgta tataaaactc ttgttttctt
301 cttttctcta aatattcttt ccttatacat taggtccttt gtagcataaa ttactatact
361 tctatagaca cgcaaacaca aatacacaca ctaaattacc ggatcaattc gggggaaaaa
421 tgctttctga aaacacgact attctgatgg ctaacggtga aattaaagac atcgcaaacg
481 tcacggctaa ctcttacgtt atgtgcgcag atggctccgc tgcccgcgtc ataaatgtca
541 cacagggcta tcagaaaatc tataatatac agcaaaaaac caaacacaga gcttttgaag
601 gtgaacctgg taggttagat cccaggcgta gaacagttta tcagcgtctt gcattacaat
661 gtactgcagg tcataaattg tcagtcaggg tccctaccaa accactgttg gaaaaaagtg
721 gtagaaatgc caccaaatat aaagtgagat ggagaaatct gcagcaatgt cagacgcttg
781 atggtaggat aataataatt ccaaaaaacc atcataagac attcccaatg acagttgaag
841 gtgagtttgc cgcaaaacgc ttcatagaag aaatggagcg ctctaaagga gaatatttca
901 actttgacat tgaagttaga gatttggatt atcttgatgc tcaattgaga atttctagct
961 gcataagatt tggtccagta ctcacaggaa atggtgtttt atctaaattt ctcactggac
1021 gtagtgacct tgtaactcct gctgtaaaaa gtatggcttg gatgcttggt ctgtggttag
1081 gtgacggtac aacaaaagag ccagaaatct cagtagatag cttggatcct aagctaatgg
1141 agagtttaag agaaaatgcg aaaatctggg gtctctacct tacggtttgt gacgatcacg
1201 ttccgctacg tgccaaacat gtaaggcttc attatggaga tggtccagat gaaaacagga
1261 agacaaggaa tttgaggaaa aataatccat tctggaaagc tgtcacaatt ttaaagttta
1321 aaagggatct tgatggagag aagcaaatcc ctgaatttat gtacggcgag catatagaag
1381 ttcgtgaagc attcttagcc ggcttgatcg actcagatgg gtacgttgtg aaaaagggcg
1441 aaggccctga atcttataaa atagcaattc aaactgttta ttcatccatt atggacggaa
1501 ttgtccatat ttcaagatct cttggtatgt cagctactgt gacgaccagg tcagctaggg
1561 aggaaatcat tgaaggaaga aaagtccaat gtcaatttac atacgactgt aatgttgctg
1621 ggggaacaac tttacagaat gttttgtcat attgtcgaag tggtcacaaa acaagagaag
1681 ttccgccaat tataaaaagg gaacccgtat atttcagctt cacggatgat ttccagggtg
1741 agagtactgt atatgggctt acgatagaag gccataaaaa tttcttgctt ggcaacaaaa
1801 tagaagtgaa atcatgtcga ggctgctgtg tgggagaaca gcataaaata tcacaaaaaa
1861 agaatctaaa acactgtgtt gcttgtccca gaaagggaat caagtatttt tataaagatt
1921 ggagtggtaa aaatcgagta tgtgctagat gctatggaag atacaaattc agcggtcatc
1981 actgtataaa ttgcaagtat gtaccagaag cacgtgaagt gaaaaaggca aaagacaaag
2041 gcgaaaaatt gggcattacg cccgaaggtt tgccagttaa aggaccagag tgtataaaat
2101 gtggcggaat cttacagttt gatgctgtcc gcgggcctca taagagttgt ggtaacaacg
2161 caggtgcgcg catctgccgg atccccgggt taattaacgg tggaggtgga ggtggaggtg
2221 gaggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag
2281 aagaagactt gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg
2341 gtgaacaaaa gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag
2401 aagacttgaa cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg
2461 aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag
2521 acttgaacgg actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac
2581 aaaagctaat ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact
2641 tgaacggact cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa
2701 agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatcaatcac tagtgaattc
2761 gcgccacttc taaataagcg aatttcttga gggccgcatc atgtaattag ttatgtcacg
2821 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
2881 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
2941 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
3001 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc aagcttggcg
3061 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
3121 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
3181 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
3241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
3301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
3361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
3421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
3481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
3541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
3601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
3661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
3721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
3781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
3841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
3901 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
3961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
4021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
4081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
4141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
4201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
4261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
4321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
4381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
4441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
4501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
4561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
4621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
4681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
4741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
4801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
4861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
4921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
4981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
5041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
5101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
5161 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
5221 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
5281 ccctttcgtc ttcaagaatt cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg
5341 gttaattaag gcgcgccaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca
5401 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
5461 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
5521 tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg
5581 agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga
5641 gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa
5701 tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg gtaaggaaaa
5761 gactcacgtt tcgaggccgc gattgaattc caacatggat gctgatttat atgggtataa
5821 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
5881 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
5941 tgagatggtc agactaaact ggctgacgga attcatgcct cttccgacca tcaagcattt
6001 tatccgtact cctgatgatg catggttact caccactgcg atccccggca aaacagcatt
6061 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
6121 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
6181 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
6241 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
6301 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
6361 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
6421 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
6481 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
6541 cgatgagttt ttctaatcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca
6601 tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat
6661 attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata
6721 tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc
6781 cgccatccag tgtcgaaaac gagctcgaat tcatcgatga tatcagatcc actagtggcc
6841 tatgcaattc tcatgtttga cagcttatca tcgatcgtcc aactgcatgg agatgagtcg
6901 tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa
6961 gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt
7021 gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt
7081 ggaaggcaag agagccccga gagcttacat tttatgttag ctggtggact gacgccagaa
7141 aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg
7201 gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa
7261 taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgcct gcaagccttt
7321 tgaaaagcaa gcataaaaga tctaaacata aaatctgtaa aataacaaga tgtaaagata
7381 atgctaaatc atttggcttt ttgattgatt gtacaggaaa atatacatcg cagggggttg
7441 acttttacca tttcaccgca atggaatcaa acttgttgaa gagaatgttc acaggcgcat
7501 acgctacaat gacccgattc ttgctagcct tttctcggtc ttgcaaacaa ccgccggcag
7561 cttagtatat aaatacacat gtacatacct ctctccgtat cctcgtaatc attttcttgt
7621 atttatcgtc ttttcgctgt aaaaacttta tcacacttat ctcaaataca cttattaacc
7681 gcttttacta ttatcttcta cgctgacagt aatatcaaac agtgacacat attaaacaca
7741 gtggtttctt tgcataaaca ccatcagcct caagtcgtca agtaaagatt tcgtgttcat
7801 gcagatagat aacaatctat atgttgataa ttagcgttgc ctcatcaatg cgagatccgt
7861 ttaaccggac cctagtgcac ttaccccacg ttcggtccac tgtgtgccga acatgctcct
7921 tcactatttt aacatgtgga attatcgatg ccggtgttat tcttgctatg ataggccatc
7981 gaatgttaaa gggaagattt attgttttgc cctttaaaga cgaatttgga ccgtgaatgt
8041 ttaaataatt ctcatgtttg acagcttatc atcgaactct aagaggtgat acttatttac
8101 tgtaaaactg tgacgataaa accggaagga agaataagaa aactcgaact gatctataat
8161 gcctattttc tgtaaagagt ttaagctatg aaagcctcgg cattttggcc gctcctaggt
8221 agtgcttttt ttccaaggac aaaacagttt ctttttcttg agcaggtttt atgtttcggt
8281 aatcataaac aataaataaa ttatttcatt tatgtttaaa aataaaaaat aaaaaagtat
8341 tttaaatttt taaaaaagtt gattataagc atgtgacctt ttgcaagcaa ttaaattttg
8401 caatttgtga tttaggcaaa agttactatt tctggctcgt gtaatatatg tatgctaatg
8461 tgaactttta caaagtcgat atggacttag tcaaaagaaa ttttcttaaa aatatatagc
8521 actagccaat ttagcacttc tttatgagat atattataga ctttattaag ccagatttgt
8581 gtattatatg tatttacccg gcgaatcatg gacatacatt ctgaaatagg taatattctc
8641 tatggtgaga cagcatagat aacctaggat acaagttaaa agctagtact gttttgcagt
8701 aatttttttc ttttttataa gaatgttacc acctaaataa gttataaagt caatagttaa
8761 gtttgatatt tgattgtaaa ataccgtaat atatttgcat gatcaaaagg ctcaatgttg
8821 actagccagc atgtcaacca ctatattgat caccgatatt aggacttcca caccaactag
8881 taatatgaca ataaattcaa gatattcttc atgagaatgg cccagcgatt atgcggtgtg
8941 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
9001 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
9061 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
9121 gttgtaaaac gacggccagt gaattcttat tacgatcctc gcgcccccta cccaccgtac
9181 tcgtcaattc caagggcatc ggtaaacatc tgctcaaact cgaagtcggc catatccaga
9241 gcgccgtagg gggcggagtc gtggggggta aatcccggac ccggggaatc cccgtccccc
9301 aacatgtcca gatcgaaatc gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg
9361 tctaagtgga gctcgtcccc caggctgaca tcggtcgggg gggccgtcga cagtctgcgc
9421 gtgtgtcccg cggggagaaa ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg
9481 tcgtcgtccg ggagatcgag caggccctcg atggtagacc cgtaattgtt tttcgtacgc
9541 gcgcggctgt acgcggaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc
9601 aattcaaggc cgaataagaa ggctggctct gcaccttggc gatcaaataa ttcgatagct
9661 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact
9721 tgatgctctt gttcttccaa tacgcaacct aaagtaaagt gccccacagc gctgagtgca
9781 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga
9841 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga
9901 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc
9961 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggg taaggcgtcg
10021 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc
10081 tgggcgagtt tacgggttgt taaaccttcg attccgaccc cattaagcag ctctaatgcg
10141 ccgttaatca ctttactttt atctaatcta gacatatgaa ttaattcggg gccgcggagg
10201 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
10261 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg
10321 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
10381 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
10441 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
10501 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
10561 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
10621 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
10681 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
10741 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
10801 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
10861 tgtcaacatg gcggtaatgt tggacatgag ccaatataaa tgtacatatt atgatatgga
10921 tacaacgtat gcaatgggcc aagctcctcg agtaattcgc gccacttcta ataagcgatt
10981 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
11041 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
11101 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
11161 gcagatccgc tagggataac agggtaatat agatcaattc ctcgatcgag tttaccac
//
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