| 质粒名称: | pLZ345 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000616 |
| 产品用途: | Mammalian VanR-specific NanoLuc expression vector (VanO5-PhCMVmin-NanoLuc-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | VanO5-PhCMVmin |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NanoLuc |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ345
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ345载体质粒基本信息
pLZ345质粒图谱载体图谱和pLZ345载体序列质粒序列多克隆位点信息
pLZ345质粒载体简介
pLZ345质粒序列载体序列
LOCUS pLZ345 5530 bp DNA circular SYN 13-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5530)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5530
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 228
/label=E2F-1
misc_feature 234..245
/label=VanO
/label=nonstandard type: operator
misc_feature 248..259
/label=VanO
/label=nonstandard type: operator
misc_feature 262..273
/label=VanO
/label=nonstandard type: operator
misc_feature 276..287
/label=VanO
/label=nonstandard type: operator
misc_feature 290..301
/label=VanO
/label=nonstandard type: operator
misc_feature 302..306
/label=E2F-1
misc_feature 307..455
/label=hcmvmin
promoter 329..367
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 377..391
/label=New Feature
misc_feature 474..542
/label=Secretory (IgK)
/label=nonstandard type: signal
CDS 561..1076
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
misc_feature 594..596
/label=cyt-STOP (TGG->TGA)
terminator 1124..1351
/label=bGH PA
rep_origin 1397..1825
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1839..2168
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2235..3029
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2279..2306)
/label=188-WKG-610-R
polyA_signal 3203..3324
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3369..3389)
/label=M13-rev
primer_bind complement(3373..3389)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(3395..3417)
/label=LacO
protein_bind 3397..3413
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3421..3451)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(3422..3451)
/label=lac
protein_bind 3466..3487
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 3593..4533
/label=ColE1
/note="ColE1"
CDS complement(4534..5394)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5395..5499)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtattggat
241 ccaatgcatt ggatccaatg gattggatcc aatcgattgg atccaattga ttggatccaa
301 tctagctcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
361 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
421 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgcca cctatgacta
481 gtgagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt tccactggtg
541 acgctagtgg tggttctggt atggtcttca cactcgaaga tttcgttggg gactggcgac
601 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
661 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
721 tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc
781 agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc
841 tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac
901 ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt
961 ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc
1021 gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaagcgg
1081 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
1141 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
1201 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
1261 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
1321 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
1381 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
1441 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
1501 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
1561 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
1621 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
1681 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
1741 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
1801 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
1861 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
1921 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
1981 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
2041 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
2101 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
2161 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
2221 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
2281 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
2341 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
2401 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
2461 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
2521 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
2581 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
2641 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
2701 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
2761 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
2821 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
2881 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
2941 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
3001 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
3061 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
3121 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
3181 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
3241 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
3301 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
3361 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
3421 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
3481 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
3541 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
3601 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
3661 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
3721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
3781 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
3841 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
3901 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
3961 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
4021 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
4081 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
4141 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
4201 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
4261 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
4321 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
4381 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
4441 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
4501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
4561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
4621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
4681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
4741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
4801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
4861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
4921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
4981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
5041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
5101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
5161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
5221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
5281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
5341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
5401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
5461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
5521 acctgacgtc
//
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